In Vitro Antifungal Susceptibility and Molecular Characterization of Clinical Isolates of Fusarium verticillioides (F. moniliforme) and Fusarium thapsinum

ABSTRACT A microdilution method was used to test 11 antifungal drugs against clinical isolates of Fusarium thapsinum and three different phylogenetic clades of Fusarium verticillioides that were characterized by sequencing a region of the β-tubulin gene. Terbinafine was the most-active drug against both species, followed by posaconazole against F. verticillioides.

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In Vitro Antifungal Susceptibility Of Griseofulvin, Fluconazole, Itraconazole And Terbinafine Against Clinical Isolates Of Trichophyton Rubrum And Trichophyton Mentagrophytes

Asian Journal of Health Sciences ◽

10.15419/ajhs.v2i1.420 ◽

2014 ◽

Vol 2 (1) ◽

Author(s):

K R Reddy ◽

S Ram Reddy

Keyword(s):

Trichophyton Rubrum ◽

Clinical Laboratory ◽

Antifungal Susceptibility ◽

Trichophyton Mentagrophytes ◽

Drug Susceptibility ◽

Antifungal Drugs ◽

Clinical Isolates ◽

National Committee ◽

In Vitro Antifungal Susceptibility

Investigations on antifungal drug susceptibility were carried out on 90 clinical isolates of Trichophyton rubrum, and Trichophyton mentagrophytes with four antifungal drugs, namely griseofulvin, fluconazole, itraconazole and terbinafine as suggested by National Committee for Clinical Laboratory Standards (NCCLS) M27–A (1997) document by broth macrodilution method to standardize in vitro antifungal susceptibility testing and to find out the Minimum Inhibitory Concentration (MIC) of the drugs. In this study, terbinafine was found to be the most efficient drug for all isolates. Terbinafine had the lowest MIC range of 0.001 g/ml to 0.09 g/ml and MIC50 was low at 0.005 g/ml and MIC90 was also low at 0.04 g/ml against T.rubrum; and MIC range of 0.001μg/ml to 0.19μg/ml with a MIC50 of 0.01μg/ml and MIC90 at 0.09μg/ml against T.mentagrophytes. Itraconazole showed antifungal activity superior to that of fluconazole, with a MIC range of 0.04g/ml to 1.56g/ml, with MIC50 at 0.19μg/ml and MIC90 at 1.56g/ml against T.rubrum; and MIC range of 0.04μg/ml to 1.56μg/ml, with MIC50 at 0.19μg/ml and MIC90 at 0.78μg/ml against T.mentagrophytes. Griseofulvin appears to be still a potent drug for management of dermatophytoses. Griseofulvin had a MIC range of 0.15g/ml to 5.07 g/ml with MIC50 at1.26 g/ml and MIC90 at 2.53 g/ml against T.rubrum; and MIC range of 0.31μg/ml to 5.07μg/ml with MIC50 at 1.26μg/ml and MIC90 at 2.53μg/ml against T.mentagrophytes. Fluconazole showed a high MIC range of 0.19 g/ml to 50 g/ml and MIC50 was high at 1.56g/ml and MIC90 was also high at 12.5 g/ml against T.rubrum; and a high MIC range of 0.09μg/ml to 25.0μg/ml, with MIC50 at 1.56μg/ml and MIC90 at 12.5μg/ml towards T.mentagrophytes. The technique was found to be easy to perform and reliable with consistent results.

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In Vitro Antifungal Susceptibility Profiles of 12 Antifungal Drugs against 55 Trichophyton schoenleinii Isolates from Tinea Capitis Favosa Patients in Iran, Turkey, and China

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01753-16 ◽

2016 ◽

Vol 61 (2) ◽

Cited By ~ 6

Author(s):

Shuwen Deng ◽

Saham Ansari ◽

Macit Ilkit ◽

Haleh Rafati ◽

Mohammad T. Hedayati ◽

...

Keyword(s):

Antifungal Agents ◽

Antifungal Susceptibility ◽

Antifungal Drugs ◽

Content Type ◽

Microdilution Method ◽

Broth Microdilution Method ◽

In Vitro Antifungal Susceptibility ◽

Trichophyton Schoenleinii ◽

Susceptibility Profiles

ABSTRACT Trichophyton schoenleinii is an anthropophilic dermatophyte mainly causing tinea favosa of the scalp in certain regions of the world, especially Africa and Asia. We investigated the in vitro susceptibilities of 55 T. schoenleinii isolates collected over the last 30 years from Iran, Turkey, and China to 12 antifungals using the CLSI broth microdilution method. Our results revealed that terbinafine and ketoconazole were the most potent antifungal agents among those tested, independently of the geographic regions where strains were isolated.

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Species Distribution and In Vitro Antifungal Susceptibility Patterns of 75 Clinical Isolates of Fusarium spp. from Northern Italy

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00272-08 ◽

2008 ◽

Vol 52 (7) ◽

pp. 2683-2685 ◽

Cited By ~ 56

Author(s):

Anna Maria Tortorano ◽

Anna Prigitano ◽

Giovanna Dho ◽

Maria Carmela Esposto ◽

Claudia Gianni ◽

...

Keyword(s):

Species Distribution ◽

Fusarium Verticillioides ◽

Antifungal Susceptibility ◽

Northern Italy ◽

Molecular Methods ◽

Clinical Isolates ◽

Fusarium Spp ◽

In Vitro Antifungal Susceptibility ◽

Susceptibility Patterns

ABSTRACT Fusarium isolates from 75 Italian patients were identified by molecular methods, and their susceptibilities to antifungals were tested in vitro. Fusarium verticillioides was the species most frequently isolated from deep-seated infections, and F. solani was the species most frequently isolated from superficial infections. F. solani isolates showed high azole MICs, while F. verticillioides isolates showed low posaconazole MICs.

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In vitro antifungal susceptibility of griseofulvin, fluconazole, itraconazole and terbinafine against clinical isolates of Trichophyton rubrum and Trichophyton mentagrophytes

Journal of Universal College of Medical Sciences ◽

10.3126/jucms.v1i4.9569 ◽

2014 ◽

Vol 1 (4) ◽

pp. 26-28

Author(s):

KR Reddy ◽

SR Reddy

Keyword(s):

Trichophyton Rubrum ◽

Clinical Laboratory ◽

Antifungal Susceptibility ◽

Trichophyton Mentagrophytes ◽

Drug Susceptibility ◽

Antifungal Drugs ◽

Clinical Isolates ◽

National Committee ◽

In Vitro Antifungal Susceptibility

Investigations on antifungal drug susceptibility were carried out on 90 clinical isolates of Trichophyton rubrum, and Trichophyton mentagrophytes with four antifungal drugs, namely griseofulvin, fluconazole, itraconazole and terbinafine as suggested by National Committee for Clinical Laboratory Standards (NCCLS) M27A (1997) document by broth macrodilution method to standardize in vitro antifungal susceptibility testing and to find out the Minimum Inhibitory Concentration (MIC) of the drugs. In this study, terbinafine was found to be the most efficient drug for all isolates. Terbinafine had the lowest MIC range of 0.001 g/mlto 0.09 g/ml and MIC50 was low at 0.005 g/ml and MIC90 was also low at 0.04 g/ml against T. rubrum; and MIC range of 0.001pg/ml to 0.19pg/ml with a MIC50 of 0.01pg/ml and MIC90 at 0.09 pg/ml against T. mentagrophytes. Itraconazole showed antifungal activity superior to that of fluconazole, with a MIC range of 0.04g/ml to l.56g/ml, with MIC50 at 0.19pg/m land MIC90 at l.56g/ml against T. rubrum; and MIC range of 0.04.g/ml to 1.56pg/ml, with MIC50 at 0.19pg/ml and MIC90 at 0.78pg/ml against T. mentagrophytes. Griseofulvin appears to be still a potent drug for management of dermatophytoses. Griseofulvin had a MIC range of 0.15g/ml to 5.07 g/ml with MIC50 at l.26 g/ml and MIC90 at 2.53 g/ml against T. rubrum; and MIC range of 0.31pg/ml to 5.07pg/ml with MIC50 at 1.26pg/ml and MIC90 at 2.53pg/ml against T. mentagrophytes. Fluconazole showed a high MIC range of 0.19 g/ml to 50 g/ml and MIC50 was high at 1.56g/ml and MIC90 was also high at 12.5 g/ml against T. rubrum; and a high MIC range of 0.09pg/ml to 25.0pg/ml, with MIC50 at 1.56pg/ml and MIC90 at 12.5pg/ml towards T. mentagrophytes. The technique was found to be easy to perform and reliable with consistent results.DOI: http://dx.doi.org/10.3126/jucms.v1i4.9569 Journal of Universal College of Medical Sciences (2013) Vol.1 No.04: 26-28

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In Vitro Antifungal Susceptibility Testing of Candida Isolates with the EUCAST Methodology, a New Method for ECOFF Determination

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.02372-16 ◽

2017 ◽

Vol 61 (4) ◽

Cited By ~ 11

Author(s):

J. Meletiadis ◽

I. Curfs-Breuker ◽

J. F. Meis ◽

J. W. Mouton

Keyword(s):

Susceptibility Testing ◽

Antifungal Susceptibility ◽

Antifungal Drugs ◽

Second Derivative ◽

Wild Type ◽

Content Type ◽

Microdilution Method ◽

In Vitro Antifungal Susceptibility ◽

Resistance Rates

ABSTRACT The in vitro susceptibilities of 1,099 molecularly identified clinical Candida isolates against 8 antifungal drugs were determined using the EUCAST microdilution method. A new simple, objective, and mathematically solid method for determining epidemiological cutoff values (ECOFFs) was developed by derivatizing the MIC distribution and determining the derivatized ECOFF (dECOFF) as the highest MIC with the maximum second derivative. The dECOFFs were similar (95% agreement within 1 dilution) to the EUCAST ECOFFs. Overall, low non-wild-type/resistance rates were found. The highest rates were found for azoles with C. parapsilosis (2.7 to 9.8%), C. albicans (7%), and C. glabrata (1.7 to 2.3%) and for echinocandins with C. krusei (3.3%), C. albicans (1%), and C. tropicalis (1.7%).

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In vitro antifungal susceptibility of clinical isolates of Candida spp. obtained from patients with different predisposing factors to candidosis

Microbiological Research ◽

10.1016/j.micres.2006.08.007 ◽

2008 ◽

Vol 163 (5) ◽

pp. 579-585 ◽

Cited By ~ 14

Author(s):

Patrícia M. Pinto ◽

Rita de Cássia Botelho Weikert-Oliveira ◽

Juliana Pereira Lyon ◽

Verônica F. Cury ◽

Rodrigo R. Arantes ◽

...

Keyword(s):

Antifungal Susceptibility ◽

Predisposing Factors ◽

Clinical Isolates ◽

Candida Spp ◽

In Vitro Antifungal Susceptibility

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Antifungal Susceptibility of Dermatophytes Isolated From Cutaneous Fungal Infections: The Vietnamese Experience

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2019.062 ◽

2019 ◽

Vol 7 (2) ◽

pp. 247-249

Author(s):

Chau Van Tro ◽

Khuong Ho Thi Ngoc ◽

Thuong Nguyen Van ◽

Khang Tran Hau ◽

Marco Gandolfi ◽

...

Keyword(s):

Fungal Infections ◽

Antifungal Susceptibility ◽

Antifungal Drugs ◽

Broth Microdilution ◽

Direct Examination ◽

Sensitivity Testing ◽

Microdilution Method ◽

Broth Microdilution Method ◽

Vietnamese Population

AIM: Evaluate the resistance of dermatophytes to systemic antifungal drugs in the Vietnamese population. METHODS: We enrolled 101 patients with cutaneous dermatophytosis at the Dermato-Venereology hospital in HCMC from August 2016 to March 2017. All the specimens were subjected to direct examination (10% KOH mount) and culture on Sabouraud dextrose agar. In vitro antifungal sensitivity testing was done on species isolated from a culture with broth microdilution method. RESULTS: Direct microscopy was positive for dermatophytes in all patients. However this pathogen was found in fungal cultures in only 61.38% of patients. The main causative agent isolated was Trichophyton spp. (90.3%), followed by Microsporum spp. (8%) and Epidermophyton spp. (1.7%). Trichophyton spp. Has shown resistance to fluconazole, griseofulvin, ketoconazole, and itraconazole in 92.9%, 46.4%, 5.4% and 1.8% of strains, respectively. All Microsporum spp. Strains were found resistant to fluconazole and griseofulvin while resistance to ketoconazole was demonstrated in only 20% of strains and none of them was resistant to itraconazole. Epidermophyton spp strains were all resistant to fluconazole, griseofulvin, ketoconazole while none of them was resistant to itraconazole. CONCLUSION: Based upon our results, Itraconazole shows the greatest probability of efficacy in the treatment of cutaneous dermatophytosis in Vietnamese patients.

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Genetic Diversity and In Vitro Antifungal Susceptibility of 200 Clinical and Environmental Aspergillus flavus Isolates

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.00004-17 ◽

2017 ◽

Vol 61 (5) ◽

Cited By ~ 15

Author(s):

Mojtaba Taghizadeh-Armaki ◽

Mohammad Taghi Hedayati ◽

Saham Ansari ◽

Saeed Mahdavi Omran ◽

Sasan Saber ◽

...

Keyword(s):

Amphotericin B ◽

Aspergillus Flavus ◽

Susceptibility Testing ◽

Antifungal Susceptibility ◽

Content Type ◽

Microdilution Method ◽

Microsatellite Genotype ◽

Broth Microdilution Method ◽

In Vitro Antifungal Susceptibility

ABSTRACT Aspergillus flavus has been frequently reported as the leading cause of invasive aspergillosis in certain tropical and subtropical countries. Two hundred A. flavus strains originating from clinical and environmental sources and collected between 2008 and 2015 were phylogenetically identified at the species level by analyzing partial β-tubulin and calmodulin genes. In vitro antifungal susceptibility testing was performed against antifungals using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution method. In addition, genotyping was performed using a short-tandem-repeat (STR) assay of a panel of six microsatellite markers (A. flavus 2A, 2B, 2C, 3A, 3B, and 3C), in order to determine the genetic variation and the potential relationship between clinical and environmental isolates. The geometric means of the minimum inhibitory concentrations/minimum effective concentrations (MICs/MECs) of the antifungals across all isolates were (in increasing order): posaconazole, 0.13 mg/liter; anidulafungin, 0.16 mg/liter; itraconazole, 0.29 mg/liter; caspofungin, 0.42 mg/liter; voriconazole, 0.64 mg/liter; isavuconazole, 1.10 mg/liter; amphotericin B, 3.35 mg/liter; and flucytosine, 62.97 mg/liter. All of the clinical isolates were genetically different. However, an identical microsatellite genotype was found between a clinical isolate and two environmental strains. In conclusion, posaconazole and anidulafungin showed the greatest in vitro activity among systemic azoles and echinocandins, respectively. However, the majority of the A. flavus isolates showed reduced susceptibility to amphotericin B. Antifungal susceptibility of A. flavus was not linked with the clinical or environmental source of isolation. Microsatellite genotyping may suggest an association between clinical and environmental strains, although this requires further investigation.

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