Immediate allergic reaction following intra-articular injection of ioxaglate confirmed by skin testing and flow cytometry-based basophil activation test

This work aimed to determine if it was possible to eliminate or reduce the content of the Api g1 allergen in celery juice by oxidation, utilising its natural polyphenol oxidase (PPO) content. We attempted to determine a possible relationship between the enzymatic browning of celery juice and the reactivity of the Api g1 allergen. Pressed celery juice was stirred, and samples for the colour measurement and allergenicity, determined using the Western Blot (WB) method, were collected at pre-defined times. Oxidation failed to eliminate the allergenicity of pure celery juice. Further trials were focused on celery allergen elimination in apple-celery juices mixtures in ratios of 3:1, 5:1, and 7:1. We selected the 5:1 ratio as the most acceptable from the sensory perspective, and monitored its allergenicity using the WB method, basophil activation test, and skin prick testing. The WB test showed that oxidation, caused by stirring for 120 min, reduced the allergenicity of the mixture. However, the basophil activation test showed no reduction in the allergic response to the oxidised juice mixture. Skin testing showed that the oxidised juice mixture stirred for 120 min exhibited a significantly lower reaction than the juice mixture stirred for 60 min or celery and apple juice stabilised with ascorbic acid. Due to the contradictory results in different tests, the method cannot be declared successful or safe, even for mixtures of apple-celery juices.

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Basophil activation test by flow cytometry: Present and future applications in allergology

Cytometry Part B Clinical Cytometry ◽

10.1002/cyto.b.20419 ◽

2008 ◽

Vol 74B (4) ◽

pp. 201-210 ◽

Cited By ~ 115

Author(s):

D. G. Ebo ◽

C. H. Bridts ◽

M. M. Hagendorens ◽

N. E. Aerts ◽

L. S. De Clerck ◽

...

Keyword(s):

Flow Cytometry ◽

Basophil Activation Test ◽

Basophil Activation ◽

Activation Test

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Clinical implication of detecting sensitization to iodinated radiocontrast media in the basophil activation test by flow cytometry

Russian Clinical Laboratory Diagnostics ◽

10.51620/0869-2084-2021-66-12-747-754 ◽

2021 ◽

Vol 66 (12) ◽

pp. 747-754

Author(s):

N. V. Bychkova ◽

P. A. Selivanov ◽

N. M. Kalinina

Keyword(s):

Flow Cytometry ◽

Immune Response ◽

T Lymphocytes ◽

Basophil Activation Test ◽

Basophil Activation ◽

Activation Test ◽

Radiocontrast Media ◽

Type 2 Immune Response

The use of iodinated radiocontrast media is necessary for visualization. A number of patients have adverse effects of various nature and severity when these drugs are administered. Routine allergy tests do not provide adequate diagnosis of reactions to drugs in this group. The aim of this work is to assess the capabilities of the basophil activation test to confirm sensitization to non-ionic iodinated radiocontrast media, as well as to select a safe alternative drug in patients with a burdened history. Basophil activation test by flow cytometry was performed in 184 patients The Nikiforov Russian Centre of Emergency and Radiation Medicine» EMERCOM of Russia and 32 volunteers using ultravist, omnipack, and optiray. The presence of sensitization was assessed based on the basophil activation index, as well as spontaneous and anti-IgE antibody-induced activation of basophils and the population of T-lymphocytes type 2 immune response. The volunteers showed no sensitization to iodinated radiocontrast media. In patients with a medium degree of hypersensitivity reaction in vivo, in vitro sensitization to drugs was detected 4 times more often than in patients with a mild degree (51% versus 13.5%). In patients with systemic reactions to the administration of a known drug, in vitro sensitization was confirmed in 86% of cases, while the frequency of detection of sensitization to drugs did not differ. Spontaneous activation of basophils in patients and type 2 T-lymphocytes were 2 times higher than in volunteers. Patients were more likely to have low (less than 30%) activation of basophils for anti-IgE antibodies. The specificity of the basophil activation test with iodinated radiocontrast media was 100% with a sensitivity of 94.1%. Most patients were able to select a non-sensitizing contrast. Inclusion in the algorithm of spontaneous and anti-IgE antibody-induced activation of basophils and a population of T-lymphocytes type 2 immune response will allow the doctor to carry out a personalized approach to the management of patients with a burdened history.

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