The Plio-Pleistocene planktic foraminiferal sequence of theGloborotalia(Globoconella)puncticulata-inflataclade in Deep Sea Drilling Project Site 588, dated at 4.36 Ma to 0.05 Ma, records the branching history of theG. inflatalineage from the ancestralG. puncticulatalineage. The gradational nature of the divergence and the enormous morphological variability inherent in theG. inflatalineage have elicited different views on taxonomy and phylogeny of this clade. A pattern recognition technique, soft independent modeling of class analog (SIMCA), was used as an objective quantitative stratophenetic methodology to reconstruct the phylogenetic history.Typical specimens of two species,G. puncticulataandG. inflata,were identified from a stratigraphic level dated at 2.76 Ma. Principal component models were built to characterize the morphometric patterns of the two morphotypes using SIMCA. TheGloboconellaspecimens of the next lower and higher adjacent stratigraphic levels were evaluated against the models and classified into one of the two morphotypes. The newly classified specimens were then used to build new models for further tracing of lineages in lower and upper sections, respectively. Progression of such training and classification procedures through stratigraphic intervals resulted in a reconstruction of the evolutionary patterns of the two lineages. Cladogenesis gave rise to the descendant lineage,G. inflata,at about 3.5 Ma. The two co-existing species,G. inflataandG. puncticulata,differ only in size and show similarity in most characters at the beginning of their divergence. Other characters began to diverge later, at various rates. The gradients between planktic and benthic foraminiferal δ18O values show a continuous increase during the late Pliocene. The succession fromG. puncticulatatoG. inflataduring the same time correlates with the progressively increased vertical stratification in temperature of surface waters.Globorotalia puncticulatabecame extinct at 2.35 Ma when the temperature gradient further increased, corresponding to the onset of extensive glaciation in the Northern Hemisphere.