Isolation and characterization of the Aspergillus nidulans eglC gene encoding a putative β-1,3-endoglucanase

2005 ◽  
Vol 42 (7) ◽  
pp. 590-600 ◽  
Author(s):  
Chang-Jun Choi ◽  
Hee-Jeong Ju ◽  
Byung-Hyun Park ◽  
Rui Qin ◽  
Kwang-Yeop Jahng ◽  
...  
Keyword(s):  
Aspergillus Nidulans ◽  
Gene Encoding ◽  
Isolation And Characterization

scholarly journals Phosphopantetheinyl Transferase CfwA/NpgA Is Required for Aspergillus nidulans Secondary Metabolism and Asexual Development

2007 ◽  
Vol 6 (4) ◽  
pp. 710-720 ◽  
Author(s):  
Olivia Márquez-Fernández ◽  
Ángel Trigos ◽  
Jose Luis Ramos-Balderas ◽  
Gustavo Viniegra-González ◽  
Holger B. Deising ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolism ◽  
Aspergillus Nidulans ◽  
Large Scale ◽  
Fatty Acid Biosynthesis ◽  
Phosphopantetheinyl Transferase ◽  
Gene Encoding ◽  
Peptide Synthetases ◽  
Isolation And Characterization

ABSTRACT Polyketide synthases (PKSs) and/or nonribosomal peptide synthetases (NRPSs) are central components of secondary metabolism in bacteria, plants, and fungi. In filamentous fungi, diverse PKSs and NRPSs participate in the biosynthesis of secondary metabolites such as pigments, antibiotics, siderophores, and mycotoxins. However, many secondary metabolites as well as the enzymes involved in their production are yet to be discovered. Both PKSs and NRPSs require activation by enzyme members of the 4′-phosphopantetheinyl transferase (PPTase) family. Here, we report the isolation and characterization of Aspergillus nidulans strains carrying conditional (cfwA2) and null (ΔcfwA) mutant alleles of the cfwA gene, encoding an essential PPTase. We identify the polyketides shamixanthone, emericellin, and dehydroaustinol as well as the sterols ergosterol, peroxiergosterol, and cerevisterol in extracts from A. nidulans large-scale cultures. The PPTase CfwA/NpgA was required for the production of these polyketide compounds but dispensable for ergosterol and cerevisterol and for fatty acid biosynthesis. The asexual sporulation defects of cfwA, ΔfluG, and ΔtmpA mutants were not rescued by the cfwA-dependent compounds identified here. However, a cfwA2 mutation enhanced the sporulation defects of both ΔtmpA and ΔfluG single mutants, suggesting that unidentified CfwA-dependent PKSs and/or NRPSs are involved in the production of hitherto-unknown compounds required for sporulation. Our results expand the number of known and predicted secondary metabolites requiring CfwA/NpgA for their biosynthesis and, together with the phylogenetic analysis of fungal PPTases, suggest that a single PPTase is responsible for the activation of all PKSs and NRPSs in A. nidulans.

Isolation and characterization of an Aspergillus nidulans gene encoding an alkaline protease

Gene ◽  
1994 ◽  
Vol 150 (2) ◽  
pp. 287-292 ◽  
Author(s):  
Margaret E. Katz ◽  
Robert N. Ricea ◽  
Brian F. Cheetham
Keyword(s):  
Aspergillus Nidulans ◽  
Alkaline Protease ◽  
Gene Encoding ◽  
Isolation And Characterization

Isolation and characterization of a bovine gene encoding phenol sulfotransferase

Gene ◽  
1996 ◽  
Vol 174 (2) ◽  
pp. 221-224 ◽  
Author(s):  
Travis Henry ◽  
Brian Kliewer ◽  
Robert Palmatier ◽  
Joseph S. Ulphani ◽  
Joe D. Beckmann
Keyword(s):  
Gene Encoding ◽  
Bovine Gene ◽  
Isolation And Characterization ◽  
Phenol Sulfotransferase

Isolation and characterization of the gene encoding the muscle-specific isozyme of human phosphoglycerate mutase

Gene ◽  
1990 ◽  
Vol 91 (2) ◽  
pp. 225-232 ◽  
Author(s):  
Judit Castella-Escola ◽  
David M. Ojcius ◽  
Philippe LeBoulch ◽  
Virginie Joulin ◽  
Yves Blouquit ◽  
...  
Keyword(s):  
Phosphoglycerate Mutase ◽  
Gene Encoding ◽  
Isolation And Characterization

scholarly journals Isolation and Characterization of the First Temperate Virus Infecting Psychrobacillus from Marine Sediments

Viruses ◽  
2022 ◽  
Vol 14 (1) ◽  
pp. 108
Author(s):  
Wang Liu ◽  
Xiaowei Zheng ◽  
Xin Dai ◽  
Zhenfeng Zhang ◽  
Wenyan Zhang ◽  
...  
Keyword(s):  
Marine Sediments ◽  
Hydrothermal Vent ◽  
Okinawa Trough ◽  
Marine Ecosystem ◽  
Genome Replication ◽  
Gene Encoding ◽  
Significant Similarity ◽  
Host Chromosome ◽  
Isolation And Characterization

Viruses are far more abundant than cellular microorganisms in the marine ecosystem. However, very few viruses have so far been isolated from marine sediments, especially hydrothermal vent sediments, hindering the understanding of the biology and ecological functions of these tiny organisms. Here, we report the isolation and characterization of a temperate bacteriophage, named PVJ1, which infects Psychrobacillus from a hydrothermal vent field in Okinawa Trough. PVJ1 belongs to the Myoviridae family of the order Caudovirales. The tailed phage possesses a 53,187 bp linear dsDNA genome, with 84 ORFs encoding structural proteins, genome replication, host lysis, etc. in a modular pattern. The phage genome is integrated into the host chromosome near the 3′-end of deoD, a gene encoding purine nucleoside phosphorylase (PNP). The phage integration does not appear to disrupt the function of PNP. The phage DNA is packaged by the headful mechanism. Release of PVJ1 from the host cell was drastically enhanced by treatment with mitomycin C. Phages encoding an MCP sharing significant similarity (≥70% identical amino acids) with that of PVJ1 are widespread in diverse environments, including marine and freshwater sediments, soils, artificial ecosystems, and animal intestines, and primarily infect Firmicutes. These results are valuable to the understanding of the lifestyle and host interactions of bacterial viruses at the bottom of the ocean.

Sign in / Sign up

Export Citation Format

Share Document