Genetic characterization of the Neurospora crassa molybdenum cofactor biosynthesis

2014 ◽  
Vol 66 ◽  
pp. 69-78 ◽  
Author(s):  
Corinna Probst ◽  
Phillip Ringel ◽  
Verena Boysen ◽  
Lisette Wirsing ◽  
Mariko Matsuda Alexander ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Genetic Characterization ◽  
Molybdenum Cofactor ◽  
Cofactor Biosynthesis ◽  
Molybdenum Cofactor Biosynthesis

scholarly journals The First Step of Neurospora crassa Molybdenum Cofactor Biosynthesis: Regulatory Aspects under N-Derepressing and Nitrate-Inducing Conditions

2020 ◽  
Vol 8 (4) ◽  
pp. 534
Author(s):  
Simon Wajmann ◽  
Thomas W. Hercher ◽  
Sabine Buchmeier ◽  
Robert Hänsch ◽  
Ralf R. Mendel ◽  
...  
Keyword(s):  
Neurospora Crassa ◽  
Transcriptional Control ◽  
Molybdenum Cofactor ◽  
Transfer Reactions ◽  
Biosynthesis Pathway ◽  
Prosthetic Group ◽  
Regulatory Aspects ◽  
Cofactor Biosynthesis ◽  
Single Transcript ◽  
Molybdenum Cofactor Biosynthesis

Molybdenum cofactor (Moco) is the active site prosthetic group found in all Moco dependent enzymes, except for nitrogenase. Mo-enzymes are crucial for viability throughout all kingdoms of life as they catalyze a diverse set of two electron transfer reactions. The highly conserved Moco biosynthesis pathway consists of four different steps in which guanosine triphosphate is converted into cyclic pyranopterin monophosphate, molybdopterin (MPT), and subsequently adenylated MPT and Moco. Although the enzymes and mechanisms involved in these steps are well characterized, the regulation of eukaryotic Moco biosynthesis is not. Within this work, we described the regulation of Moco biosynthesis in the filamentous fungus Neurospora crassa, which revealed the first step of the multi-step pathway to be under transcriptional control. We found, that upon the induction of high cellular Moco demand a single transcript variant of the nit-7 gene is increasingly formed pointing towards, that essentially the encoded enzyme NIT7-A is the key player for Moco biosynthesis activity in Neurospora.

scholarly journals Characterization of MOCS1A, an Oxygen-sensitive Iron-Sulfur Protein Involved in Human Molybdenum Cofactor Biosynthesis

2004 ◽  
Vol 279 (33) ◽  
pp. 34721-34732 ◽  
Author(s):  
Petra Hänzelmann ◽  
Heather L. Hernández ◽  
Christian Menzel ◽  
Ricardo García-Serres ◽  
Boi Hanh Huynh ◽  
...  
Keyword(s):  
Molybdenum Cofactor ◽  
Iron Sulfur ◽  
Oxygen Sensitive ◽  
Cofactor Biosynthesis ◽  
Sulfur Protein ◽  
Iron Sulfur Protein ◽  
Molybdenum Cofactor Biosynthesis
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