Combining phenolic grafting and laccase-catalyzed cross-linking: Effects on structures, technofunctional properties and human immunoglobulin E binding capacity of egg white proteins

2021 ◽  
Vol 355 ◽  
pp. 129587
Author(s):  
Mingqin Li ◽  
Salwa Karboune ◽  
Lan Liu ◽  
Kelly Light ◽  
Lamia L'Hocine ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Binding Capacity ◽  
Egg White ◽  
Human Immunoglobulin ◽  
Cross Linking ◽  
Egg White Proteins

An immunoglobulin E-reactive chimeric human immunoglobulin G1 anti-idiotype inhibits basophil degranulation through cross-linking of FcɛRI with FcγRIIb

2007 ◽  
Vol 38 (2) ◽  
pp. 313-319 ◽  
Author(s):  
S. J. Wigginton ◽  
P. B. Furtado ◽  
K. L. Armour ◽  
M. R. Clark ◽  
A. Robins ◽  
...  
Keyword(s):  
Immunoglobulin E ◽  
Human Immunoglobulin ◽  
Cross Linking

Identification of Egg White Proteins and Divergence in the Regulatory Region of the Ovalbumin Gene in Avians

2016 ◽  
Vol 24 (1) ◽  
pp. 12-25 ◽  
Author(s):  
Jindong Ren ◽  
Jianhong Hu ◽  
Li Chen ◽  
Yali Liu ◽  
Xiaoqin Xu ◽  
...  
Keyword(s):  
Regulatory Region ◽  
Egg White ◽  
Egg White Proteins

scholarly journals EGG WHITE PROTEINS

1950 ◽  
Vol 184 (1) ◽  
pp. 377-383 ◽  
Author(s):  
Richard H. Forsythe ◽  
Joseph F. Foster
Keyword(s):  
Egg White ◽  
Egg White Proteins

scholarly journals Production of Recombinant Monoclonal Antibodies in the Egg White of Gene-Targeted Transgenic Chickens

Genes ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 38
Author(s):  
Takehiro Mukae ◽  
Sho Okumura ◽  
Takuma Watanobe ◽  
Kyoko Yoshii ◽  
Takahiro Tagami ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Binding Capacity ◽  
Specific Binding ◽  
Egg White ◽  
Peptide Sequence ◽  
Efficient Production ◽  
Antigen Binding ◽  
Transgenic Chicken ◽  
Bioreactor System ◽  
Transgenic Chickens

Increased commercial demand for monoclonal antibodies (mAbs) has resulted in the urgent need to establish efficient production systems. We previously developed a transgenic chicken bioreactor system that effectively produced human cytokines in egg whites using genome-edited transgenic chickens. Here, we describe the application of this system to mAb production. The genes encoding the heavy and light chains of humanized anti-HER2 mAb, linked by a 2A peptide sequence, were integrated into the chicken ovalbumin gene locus using a CRISPR/Cas9 protocol. The knock-in hens produced a fully assembled humanized mAb in their eggs. The mAb expression level in the egg white was 1.4–1.9 mg/mL, as determined by ELISA. Furthermore, the antigen binding affinity of the anti-HER2 mAb obtained was estimated to be equal to that of the therapeutic anti-HER2 mAb (trastuzumab). In addition, antigen-specific binding by the egg white mAb was demonstrated by immunofluorescence against HER2-positive and -negative cells. These results indicate that the chicken bioreactor system can efficiently produce mAbs with antigen binding capacity and can serve as an alternative production system for commercial mAbs.

scholarly journals Magnum Morphogenesis during the Natural Development of the Quail Oviduct: Analysis of Egg White Proteins and Progesterone Receptor Concentration1

1986 ◽  
Vol 35 (3) ◽  
pp. 657-666 ◽  
Author(s):  
J. F. Pageaux ◽  
C. Laugier ◽  
D. Pal ◽  
M. A. D’Almeida ◽  
D. Sandoz ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Egg White ◽  
Natural Development ◽  
Egg White Proteins

Peptic digestibility of raw and heat-coagulated hen's egg white proteins at acidic pH range

2004 ◽  
Vol 55 (8) ◽  
pp. 635-640 ◽  
Author(s):  
Kenji Yoshino ◽  
Kentaro Sakai ◽  
Yoko Mizuha ◽  
Ayako Shimizuike ◽  
Shigeru Yamamoto
Keyword(s):  
Egg White ◽  
Acidic Ph ◽  
Egg White Proteins ◽  
Hen’S Egg ◽  
Ph Range
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