Lack of glutathione peroxidase-8 in the ER impacts on lipid composition of HeLa cells microsomal membranes

2020 ◽  
Vol 147 ◽  
pp. 80-89 ◽  
Author(s):  
Valentina Bosello Travain ◽  
Giovanni Miotto ◽  
Ana-Marija Vučković ◽  
Giorgio Cozza ◽  
Antonella Roveri ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Hela Cells ◽  
Lipid Composition ◽  
Microsomal Membranes

Lipid composition and monooxygenase relationship in liver microsomal membranes from rats fed unbalanced diets

1984 ◽  
Vol 23 (1) ◽  
pp. 73-77 ◽  
Author(s):  
J.F. Narbonne ◽  
M.A. Pelissier ◽  
D. Bonnamour ◽  
C. Borin ◽  
R. Albrecht
Keyword(s):  
Lipid Composition ◽  
Microsomal Membranes

scholarly journals Effect of membrane environment on the activity and inhibitability by malonyl-CoA of the carnitine acyltransferase of hepatic microsomal membranes

1997 ◽  
Vol 322 (2) ◽  
pp. 435-440 ◽  
Author(s):  
Neil M. BROADWAY ◽  
E. David SAGGERSON
Keyword(s):  
Lipid Composition ◽  
Membrane Lipids ◽  
Specific Activity ◽  
Membrane Lipid ◽  
Microsomal Membrane ◽  
Membrane Lipid Composition ◽  
Carnitine Acyltransferase ◽  
Malonyl Coa ◽  
Microsomal Membranes ◽  
Myristoyl Coa

We have investigated the extent to which membrane environment affects the catalytic properties of the malonyl-CoA-sensitive carnitine acyltransferase of liver microsomal membranes. Arrhenius-type plots of activity were linear in the absence and presence of malonyl-CoA (2.5 μM). Sensitivity to malonyl-CoA increased with decreasing assay temperature. Partly purified enzyme displayed an increased K0.5 (substrate concentration supporting half the maximal reaction rate) for myristoyl-CoA and a reduced sensitivity to malonyl-CoA compared with the enzyme in situ in membranes. Reconstitution with liposomes of a range of compositions restored the K0.5 for myristoyl-CoA to values similar to that seen in native membranes. The lipid requirements for restoration of sensitivity to malonyl-CoA were more stringent. When animals were starved for 24 h the specific activity of carnitine acyltransferase in microsomal membrane residues was increased 3.3-fold, whereas sensitivity to malonyl-CoA was decreased to 1/2.8. When enzymes partly purified from fed and starved animals were reconstituted into crude soybean phosphatidylcholine liposomes there was no difference in sensitivity to malonyl-CoA. When partly purified enzyme from fed rats was reconstituted into liposomes prepared from microsomal membrane lipids from fed animals it was 2.2-fold more sensitive to malonyl-CoA than when reconstituted with liposomes prepared from microsomal membrane lipids from starved animals. This suggests that the physiological changes in sensitivity to malonyl-CoA are mediated via changes in membrane lipid composition rather than via modification of the enzyme protein itself. The increased specific actvity of acyltransferase observed on starvation could not be attributed to changes in membrane lipid composition.

Characterization of monolayers and liposomes that mimic lipid composition of HeLa cells

2020 ◽  
Vol 196 ◽  
pp. 111288
Author(s):  
Adrià Botet-Carreras ◽  
M. Teresa Montero ◽  
Jesús Sot ◽  
Òscar Domènech ◽  
Jordi H. Borrell
Keyword(s):  
Hela Cells ◽  
Lipid Composition

scholarly journals Glutathione peroxidase 8 is transcriptionally regulated by HIFα and modulates growth factor signaling in HeLa cells

2015 ◽  
Vol 81 ◽  
pp. 58-68 ◽  
Author(s):  
Valentina Bosello-Travain ◽  
Henry J. Forman ◽  
Antonella Roveri ◽  
Stefano Toppo ◽  
Fulvio Ursini ◽  
...  
Keyword(s):  
Growth Factor ◽  
Glutathione Peroxidase ◽  
Hela Cells ◽  
Growth Factor Signaling

Vitamin B6 Deficiency and Dietary Fats: Effects on Lipid Composition and Glutathione Peroxidase Activity in Rat Liver

2006 ◽  
Vol 50 (3) ◽  
pp. 305-312 ◽  
Author(s):  
Alessandra Bordoni ◽  
Luciana Cabrini ◽  
Mario Marchetti ◽  
Francesca Danesi ◽  
Davide Bochicchio ◽  
...  
Keyword(s):  
Glutathione Peroxidase ◽  
Rat Liver ◽  
Peroxidase Activity ◽  
Lipid Composition ◽  
Dietary Fats ◽  
Vitamin B ◽  
Glutathione Peroxidase Activity
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