scholarly journals A procedure to extract functional isolated mitochondria from small-sized human atrial samples. Application to obesity with a partial characterisation of the organelles

2020 ◽  
Vol 153 ◽  
pp. 71-79 ◽  
Author(s):  
Thibault Leger ◽  
Chrystele Jouve ◽  
Veronique Patrac ◽  
Valerie Batel ◽  
Damien Bouvier ◽  
...  
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.


1954 ◽  
Vol 211 (2) ◽  
pp. 951-961 ◽  
Author(s):  
Clark Bublitz ◽  
Eugene P. Kennedy

2000 ◽  
Vol 104 (9) ◽  
pp. 1098-1107
Author(s):  
G.M. Butler ◽  
R.B. Pearce ◽  
J.P. Ride ◽  
P.R. Ashton

1990 ◽  
Vol 170 (2) ◽  
pp. 609-615 ◽  
Author(s):  
Maria Barile ◽  
Sergio Giannattasio ◽  
Ersilia Marra ◽  
Salvatore Passarella ◽  
Piero Pucci ◽  
...  

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