Tillage influences the retention and storage of organic matter (OM) in soil. We used a sequential density and particle-size fractionation to evaluate the total quantity and distribution of organic carbon (C) and nitrogen (N) in the profile of soils under mouldboard ploughing and no-till. Cores (0-60 cm) from four long-term tillage studies (in Prince Edward Island, Quebec, and two sites in Ontario) on soils of different textures, from sandy loam to clay were collected and divided into six depth increments. Four soil OM fractions were isolated on the basis of size and density from each sample. At two sites the standing stocks of C and N were larger in tilled than in no-till soil profiles at depths slightly greater than plough depth; tillage effects were also evident at a third site, where the mass of C in a layer close to the depth of ploughing was greater in tilled soils. Ploughing also had a substantial and consistent positive effect in all four soils on the quantity of C (and in some cases N) protected within aggregates, particularly at or near the bottom of the plough layer, and sometimes in surface soil layers. We attribute differences observed in whole soil C and N due to tillage mostly to differences in mineral-associated OM, particularly in soils with heavier textures, but the other size and density fractions could, together, account for as much as 25% of the difference. Because the C capacity level was not reached in heavy-textured soils, these soils show greater potential for further accumulation of C in the soil profile than coarse-textured soils, especially near the bottom of the plough layer.Key words: Light fraction, soil organic matter, soil tillage, physically-protected organic matter, no-till, soil texture
Ecosystem type effects on the stabilization of organic matter in soils: Combining size fractionation with sequential chemical extractions
