Monitoring of perfusion quality and prediction of donor heart function during ex-vivo machine perfusion by myocardial microcirculation versus surrogate parameters

2021 ◽  
Vol 40 (5) ◽  
pp. 387-391
Author(s):  
Lars Saemann ◽  
Folker Wenzel ◽  
Matthias Kohl ◽  
Sevil Korkmaz-Icöz ◽  
Fabio Hoorn ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Heart Function ◽  
Machine Perfusion ◽  
Donor Heart ◽  
Myocardial Microcirculation

Development of a Prediction Model for Donor Heart Function Based on Myocardial Microcirculation during Ex Vivo Blood Perfusion

2021 ◽  
Author(s):  
L. Saemann ◽  
F. Wenzel ◽  
M. Kohl ◽  
S. Korkmaz-Icöz ◽  
G. Veres ◽  
...  
Keyword(s):  
Prediction Model ◽  
Ex Vivo ◽  
Heart Function ◽  
Blood Perfusion ◽  
Donor Heart ◽  
Myocardial Microcirculation

Predicting donor heart function in a heartbeat

2020 ◽  
Vol 12 (538) ◽  
pp. eabb5667
Author(s):  
Steven P. Keller
Keyword(s):  
Machine Learning ◽  
Cardiac Function ◽  
Ex Vivo ◽  
Heart Function ◽  
Donor Heart ◽  
Learning Methods ◽  
Ex Vivo Perfusion ◽  
Machine Learning Methods

Machine learning methods can assess contractility of donor hearts maintained via ex vivo perfusion to predict posttransplant cardiac function.

scholarly journals Impact of Myocardial Load on the Preservation of Donor Heart Function during Ex Vivo Perfusion

2016 ◽  
Vol 35 (4) ◽  
pp. S186 ◽  
Author(s):  
C.W. White ◽  
S. Shan ◽  
S. Hatami ◽  
V. Gurtu ◽  
A. Kinnaird ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Heart Function ◽  
Donor Heart ◽  
Ex Vivo Perfusion

scholarly journals Mesenchymal stem cell secretions improve donor heart function following ex vivo cold storage

2020 ◽  
Author(s):  
Meijing Wang ◽  
Liangliang Yan ◽  
Qianzhen Li ◽  
Yang Yang ◽  
Mark Turrentine ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cold Storage ◽  
Ex Vivo ◽  
Heart Function ◽  
Donor Heart

scholarly journals EX VIVO PERFUSION IN A LOADED STATE IMPROVES THE PRESERVATION OF DONOR HEART FUNCTION

2015 ◽  
Vol 31 (10) ◽  
pp. S202 ◽  
Author(s):  
C.W. White ◽  
S. Shan ◽  
S. Hatami ◽  
V. Gurtu ◽  
A. Kinnaird ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Heart Function ◽  
Donor Heart ◽  
Ex Vivo Perfusion ◽  
Loaded State

scholarly journals Zebrafish as a New Tool in Heart Preservation Research

2021 ◽  
Vol 8 (4) ◽  
pp. 39
Author(s):  
Luciana Da Silveira Cavalcante ◽  
Shannon N. Tessier
Keyword(s):  
New Technologies ◽  
Ex Vivo ◽  
Machine Perfusion ◽  
Life Saving ◽  
First Choice ◽  
Heart Preservation ◽  
End Stage Heart Failure ◽  
Ischemia Tolerance ◽  
The 1960S ◽  
End Stage

Heart transplantation became a reality at the end of the 1960s as a life-saving option for patients with end-stage heart failure. Static cold storage (SCS) at 4–6 °C has remained the standard for heart preservation for decades. However, SCS only allows for short-term storage that precludes optimal matching programs, requires emergency surgeries, and results in the unnecessary discard of organs. Among the alternatives seeking to extend ex vivo lifespan and mitigate the shortage of organs are sub-zero or machine perfusion modalities. Sub-zero approaches aim to prolong cold ischemia tolerance by deepening metabolic stasis, while machine perfusion aims to support metabolism through the continuous delivery of oxygen and nutrients. Each of these approaches hold promise; however, complex barriers must be overcome before their potential can be fully realized. We suggest that one barrier facing all experimental efforts to extend ex vivo lifespan are limited research tools. Mammalian models are usually the first choice due to translational aspects, yet experimentation can be restricted by expertise, time, and resources. Instead, there are instances when smaller vertebrate models, like the zebrafish, could fill critical experimental gaps in the field. Taken together, this review provides a summary of the current gold standard for heart preservation as well as new technologies in ex vivo lifespan extension. Furthermore, we describe how existing tools in zebrafish research, including isolated organ, cell specific and functional assays, as well as molecular tools, could complement and elevate heart preservation research.

scholarly journals Generation of vascular chimerism within donor organs

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shahar Cohen ◽  
Shirly Partouche ◽  
Michael Gurevich ◽  
Vladimir Tennak ◽  
Vadym Mezhybovsky ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Patient Specific ◽  
Machine Perfusion ◽  
Organ Perfusion ◽  
Perfusion Process ◽  
Hind Limbs ◽  
Porcine Organs ◽  
Immunological Barrier

AbstractWhole organ perfusion decellularization has been proposed as a promising method to generate non-immunogenic organs from allogeneic and xenogeneic donors. However, the ability to recellularize organ scaffolds with multiple patient-specific cells in a spatially controlled manner remains challenging. Here, we propose that replacing donor endothelial cells alone, while keeping the rest of the organ viable and functional, is more technically feasible, and may offer a significant shortcut in the efforts to engineer transplantable organs. Vascular decellularization was achieved ex vivo, under controlled machine perfusion conditions, in various rat and porcine organs, including the kidneys, liver, lungs, heart, aorta, hind limbs, and pancreas. In addition, vascular decellularization of selected organs was performed in situ, within the donor body, achieving better control over the perfusion process. Human placenta-derived endothelial progenitor cells (EPCs) were used as immunologically-acceptable human cells to repopulate the luminal surface of de-endothelialized aorta (in vitro), kidneys, lungs and hind limbs (ex vivo). This study provides evidence that artificially generating vascular chimerism is feasible and could potentially pave the way for crossing the immunological barrier to xenotransplantation, as well as reducing the immunological burden of allogeneic grafts.

scholarly journals NOVEL APPROACH TO IMPROVE CIRCULATORY DEATH DONOR HEART FUNCTION

2021 ◽  
Author(s):  
Mohammed Quader ◽  
Eleonora Mezzaroma ◽  
Niluka Wickramaratne ◽  
Stefano Toldo
Keyword(s):  
Heart Function ◽  
Donor Heart ◽  
Novel Approach ◽  
Circulatory Death

Implementation and design of customized ex‐vivo machine perfusion. Analysis of its first results

2021 ◽  
Author(s):  
Sergio Riveros ◽  
Carlo Marino ◽  
Gabriela Ochoa ◽  
Emilio Morales ◽  
Dagoberto Soto ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Machine Perfusion ◽  
First Results
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