Identification of HLA-G*01:17, a new allele possibly generated by an interloci gene conversion event involving exon 3 of HLA-B

Homothallic switching of the mating type genes of Saccharomyces cerevisiae occurs by a gene conversion event, replacing sequences at the expressed MAT locus with a DNA segment copied from one of two unexpressed loci, HML or HMR. The transposed Ya or Y alpha sequences are flanked by homologous regions that are believed to be essential for switching. We examined the transposition of a mating type gene (hmr alpha 1-delta 6) which contains a 150-base-pair deletion spanning the site where the HO endonuclease generates a double-stranded break in MAT that initiates the gene conversion event. Despite the fact that the ends of the cut MAT region no longer share homology with the donor hmr alpha 1-delta 6, switching of MATa or MAT alpha to mat alpha 1-delta 6 was efficient. However, there was a marked increase in the number of aberrant events, especially the formation of haploid-inviable fusions between MAT and the hmr alpha 1-delta 6 donor locus.

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A potential donor gene for the bm1 gene conversion event in the C57BL mouse

Nature ◽

10.1038/306792a0 ◽

1983 ◽

Vol 306 (5945) ◽

pp. 792-795 ◽

Cited By ~ 80

Author(s):

Andrew L. Mellor ◽

Elizabeth H. Weiss ◽

K. Ramachandran ◽

Richard A. Flavell

Keyword(s):

Gene Conversion ◽

Gene Conversion Event ◽

C57bl Mouse ◽

Potential Donor ◽

Conversion Event ◽

Donor Gene

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Homothallic switching of Saccharomyces cerevisiae mating type genes by using a donor containing a large internal deletion.

Molecular and Cellular Biology ◽

10.1128/mcb.5.8.2154 ◽

1985 ◽

Vol 5 (8) ◽

pp. 2154-2158 ◽

Cited By ~ 4

Author(s):

B Weiffenbach ◽

J E Haber

Keyword(s):

Saccharomyces Cerevisiae ◽

Gene Conversion ◽

Mating Type ◽

Gene Conversion Event ◽

Conversion Event ◽

Base Pair Deletion ◽

Mating Type Genes ◽

Type Gene ◽

Mat Locus ◽

Donor Locus

Homothallic switching of the mating type genes of Saccharomyces cerevisiae occurs by a gene conversion event, replacing sequences at the expressed MAT locus with a DNA segment copied from one of two unexpressed loci, HML or HMR. The transposed Ya or Y alpha sequences are flanked by homologous regions that are believed to be essential for switching. We examined the transposition of a mating type gene (hmr alpha 1-delta 6) which contains a 150-base-pair deletion spanning the site where the HO endonuclease generates a double-stranded break in MAT that initiates the gene conversion event. Despite the fact that the ends of the cut MAT region no longer share homology with the donor hmr alpha 1-delta 6, switching of MATa or MAT alpha to mat alpha 1-delta 6 was efficient. However, there was a marked increase in the number of aberrant events, especially the formation of haploid-inviable fusions between MAT and the hmr alpha 1-delta 6 donor locus.

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RAD52-INDEPENDENT MITOTIC GENE CONVERSION IN SACCHAROMYCES CEREVISIAE FREQUENTLY RESULTS IN CHROMOSOMAL LOSS

Genetics ◽

10.1093/genetics/111.1.7 ◽

1985 ◽

Vol 111 (1) ◽

pp. 7-22

Author(s):

James E Haber ◽

Mark Hearn

Keyword(s):

Saccharomyces Cerevisiae ◽

Gene Conversion ◽

Mitotic Recombination ◽

Gene Conversion Event ◽

Intragenic Recombination ◽

Wild Type ◽

Conversion Event ◽

Chromosomal Loss ◽

Mitotic Gene Conversion ◽

Striking Effect

ABSTRACT We have examined spontaneous, interchromosomal mitotic recombination events between his4 alleles in both Rad+ and rad52 strains of Saccharomyces cerevisiae. In Rad+ strains, 74% of the His+ prototrophs resulted from gene conversion events without exchange of flanking markers. In diploids homozygous for the rad52-1 mutation, the frequency of His+ prototroph formation was less than 5% of the wild-type value, and more than 80% of the gene conversion events were accompanied by an exchange of flanking markers. Most of the rad52 intragenic recombination events arose by gene conversion accompanied by an exchange of flanking markers and not by a simple reciprocal exchange between the his4A and his4C alleles. There were also profound effects on the kinds of recombinant products that were recovered. The most striking effect was that RAD52-independent mitotic recombination frequently results in the loss of one of the two chromosomes participating in the gene conversion event.

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