Artificial miRNA-mediated silencing of ecdysone receptor ( EcR ) affects larval development and oogenesis in Helicoverpa armigera

2016 ◽  
Vol 77 ◽  
pp. 21-30 ◽  
Author(s):  
Sneha Yogindran ◽  
Manchikatla Venkat Rajam
Keyword(s):  
Larval Development ◽  
Helicoverpa Armigera ◽  
Ecdysone Receptor ◽  
Artificial Mirna ◽  
Helicoverpa Armígera

scholarly journals Increased plant volatile production affects oviposition, but not larval development, in the moth Helicoverpa armigera

2011 ◽  
Vol 214 (21) ◽  
pp. 3672-3677 ◽  
Author(s):  
E. J. McCallum ◽  
J. P. Cunningham ◽  
J. Lucker ◽  
M. P. Zalucki ◽  
J. J. De Voss ◽  
...  
Keyword(s):  
Larval Development ◽  
Helicoverpa Armigera ◽  
Plant Volatile ◽  
Helicoverpa Armígera ◽  
Volatile Production

The effect of silencing arginine kinase by RNAi on the larval development of Helicoverpa armigera

2015 ◽  
Vol 105 (5) ◽  
pp. 555-565 ◽  
Author(s):  
X.-L. Qi ◽  
X.-F. Su ◽  
G.-Q. Lu ◽  
C.-X. Liu ◽  
G.-M. Liang ◽  
...  
Keyword(s):  
Larval Development ◽  
Helicoverpa Armigera ◽  
Developmental Stages ◽  
Larval Mortality ◽  
Arginine Kinase ◽  
Polymorphism Analysis ◽  
Kinase Gene ◽  
Frame Sequence ◽  
Rnai Technology ◽  
Helicoverpa Armígera

AbstractArginine kinase (AK) is an important regulation factor of energy metabolism in invertebrate. An arginine kinase gene, named HaAK, was identified to be differentially expressed between Cry1Ac-susceptible (96S) and Cry1Ac-resistant (Bt-R) Helicoverpa armigera larvae using cDNA-amplification fragment length polymorphism analysis. The full-length open reading frame sequence of HaAK gene with 1068 bp was isolated from H. armigera. Quantitative reverse transcription polymerase chain reaction assay revealed that HaAK gene is specifically expressed in multiple tissues and at larval developmental stages. The peak expression level of HaAK was detected in the midgut of the fifth-instar larvae. Moreover, the expression of HaAK was obviously down-regulated in Bt-R larvae. We further constructed a dsRNA vector directly targeting HaAK and employed RNAi technology to control the larvae. The feeding bioassays showed that minute quantities of dsRNA could greatly increase the larval mortality and delay the larval pupation. Silencing of HaAK significantly retarded the larval development, indicating that HaAK is a potential target for RNA interference-based pest management.

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