Host-derived artificial miRNA-mediated silencing of ecdysone receptor gene provides enhanced resistance to Helicoverpa armigera in tomato

Genomics ◽  
2020 ◽  
Author(s):  
Sneha Yogindran ◽  
Manchikatla Venkat Rajam
Keyword(s):  
Helicoverpa Armigera ◽  
Receptor Gene ◽  
Ecdysone Receptor ◽  
Artificial Mirna ◽  
Enhanced Resistance ◽  
Helicoverpa Armígera

A fructose receptor gene influences development and feed intake in Helicoverpa armigera

2021 ◽  
Author(s):  
Dong Ai ◽  
Chenxi Dong ◽  
Bin Yang ◽  
Caihong Yu ◽  
Guirong Wang
Keyword(s):  
Helicoverpa Armigera ◽  
Feed Intake ◽  
Receptor Gene ◽  
Helicoverpa Armígera

scholarly journals Expressing Double-Stranded RNAs of Insect Hormone-Related Genes Enhances Baculovirus Insecticidal Activity

2019 ◽  
Vol 20 (2) ◽  
pp. 419 ◽  
Author(s):  
Zheming Liu ◽  
Xiaofang Wang ◽  
Yan Dai ◽  
Xiaoli Wei ◽  
Mi Ni ◽  
...  
Keyword(s):  
Helicoverpa Armigera ◽  
Insecticidal Activity ◽  
Target Genes ◽  
Receptor Gene ◽  
Mrna Level ◽  
Recombinant Baculovirus ◽  
Cotton Bollworm ◽  
Cotton Production ◽  
Control Value ◽  
Helicoverpa Armígera

Baculoviruses have already been used for insect pest control, but the slow killing speed limits their further promotion and application. Here we provide a strategy for improving baculovirus insecticidal activity using Helicoverpa armigera nucleopolyhedrovirus (HearNPV) to express double-stranded RNAs (dsRNAs) targeting cotton bollworm (Helicoverpa armigera) juvenile hormone (JH)-related genes. Droplet-feeding bioassays show that the 50% lethal concentration (LC50) values of recombinant baculoviruses expressing the dsRNA of JH acid methyl transferase gene (HaJHAMT) and the JH acid binding protein gene (HaJHBP) were 1.24 × 104 polyhedral inclusion bodies (PIB)/mL and 2.26 × 104 PIB/mL, respectively. Both were much lower than the control value (8.12 × 104 PIB/mL). Meanwhile, the LT50 of recombinant baculovirus expressing dsRNA of HaJHBP was only 54.2% of the control value, which means that larval death was accelerated. Furthermore, the mRNA level of target genes was reduced in recombinant baculovirus-treated cotton bollworm larvae. Transcription of several key genes involved in hormone signaling pathways—for example, ecdysone receptor gene (HaEcR)—was also altered. This study establishes a new strategy for pest management by interfering with insect hormone-related gene expression via baculoviruses, and the engineered baculoviruses have great potential application in cotton production.

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