Crystal structure of the blue fluorescent protein with a Leu-Leu-Gly tri-peptide chromophore derived from the purple chromoprotein of Stichodactyla haddoni

2019 ◽  
Vol 130 ◽  
pp. 675-684
Author(s):  
Hsin-Yang Chang ◽  
Tzu-Ping Ko ◽  
Yu-Ching Chang ◽  
Kai-Fa Huang ◽  
Cheng-Yung Lin ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Fluorescent Protein ◽  
Blue Fluorescent Protein ◽  
Stichodactyla Haddoni

scholarly journals The structure of a GFP-based antibody (fluorobody) to TLH, a toxin fromVibrio parahaemolyticus

2015 ◽  
Vol 71 (7) ◽  
pp. 913-918 ◽  
Author(s):  
Yaoguang Chen ◽  
Xiaocheng Huang ◽  
Rongzhi Wang ◽  
Shihua Wang ◽  
Ning Shi
Keyword(s):  
Crystal Structure ◽  
Green Fluorescent Protein ◽  
Vibrio Parahaemolyticus ◽  
Fluorescent Protein ◽  
Hybrid Molecule ◽  
Unit Cell Parameters ◽  
Cell Parameters ◽  
Food Borne ◽  
Vapour Diffusion ◽  
Green Fluorescent

A fluorobody is a manmade hybrid molecule that is composed of green fluorescent protein (GFP) and a fragment of antibody, which combines the affinity and specificity of an antibody with the visibility of a GFP. It is able to provide a real-time indication of binding while avoiding the use of tags and secondary binding reagents. Here, the expression, purification and crystal structure of a recombinant fluorobody for TLH (thermolabile haemolysin), a toxin from the lethal food-borne disease bacteriumVibrio parahaemolyticus, are presented. This is the first structure of a fluorobody to be reported. Crystals belonging to space groupP43212, with unit-cell parametersa=b= 63.35,c = 125.90 Å, were obtained by vapour diffusion in hanging drops and the structure was refined to anRfreeof 16.7% at 1.5 Å resolution. The structure shows a CDR loop of the antibody on the GFP scaffold.

Structure-Guided Generation of a Redox-Independent Blue Fluorescent Protein from mBFP

2019 ◽  
Vol 431 (17) ◽  
pp. 3191-3202
Author(s):  
Pil-Won Seo ◽  
Eun-Seo Jo ◽  
Sung-Hwan You ◽  
Dae-Eun Cheong ◽  
Geun-Joong Kim ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Blue Fluorescent Protein

Cloning and Characterization of a Blue Fluorescent Protein from Vibrio vulnificus

2001 ◽  
Vol 287 (2) ◽  
pp. 359-365 ◽  
Author(s):  
Jer-Horng Su ◽  
Yin-Ching Chuang ◽  
Ying-Chieh Tsai ◽  
Ming-Chung Chang
Keyword(s):  
Fluorescent Protein ◽  
Vibrio Vulnificus ◽  
Blue Fluorescent Protein

A BLUE FLUORESCENT PROTEIN FROM A YELLOW-EMITTING LUMINOUS BACTERIUM

1992 ◽  
Vol 55 (2) ◽  
pp. 293-299 ◽  
Author(s):  
Hajime Karatani ◽  
Thérèse Wilson ◽  
J. Woodland Hastings
Keyword(s):  
Fluorescent Protein ◽  
Luminous Bacterium ◽  
Blue Fluorescent Protein

Photophysics of the blue fluorescent protein

2007 ◽  
Vol 122-123 ◽  
pp. 291-293 ◽  
Author(s):  
K. Mauring ◽  
V. Krasnenko ◽  
S. Miller
Keyword(s):  
Fluorescent Protein ◽  
Blue Fluorescent Protein
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