Serine carboxypeptidases from the carnivorous plant Nepenthes mirabilis: Partial characterization and heterologous expression

Author(s):  
Camila T.M.N. Porfírio ◽  
Pedro F.N. Souza ◽  
Márcio V. Ramos ◽  
Francisco A.P. Campos ◽  
Samuel F. Freitas ◽  
...  
2014 ◽  
Vol 100 ◽  
pp. 43-50 ◽  
Author(s):  
Franziska Buch ◽  
Yannick Pauchet ◽  
Matthias Rott ◽  
Axel Mithöfer

Author(s):  
B. J. Panessa ◽  
J. F. Gennaro

Tissue from the hood and sarcophagus regions were fixed in 6% glutaraldehyde in 1 M.cacodylate buffer and washed in buffer. Tissue for SEM was partially dried, attached to aluminium targets with silver conducting paint, carbon-gold coated(100-500Å), and examined in a Kent Cambridge Stereoscan S4. Tissue for the light microscope was post fixed in 1% aqueous OsO4, dehydrated in acetone (4°C), embedded in Epon 812 and sectioned at ½u on a Sorvall MT 2 ultramicrotome. Cross and longitudinal sections were cut and stained with PAS, 0.5% toluidine blue and 1% azure II-methylene blue. Measurements were made from both SEM and Light micrographs.The tissue had two structurally distinct surfaces, an outer surface with small (225-500 µ) pubescent hairs (12/mm2), numerous stoma (77/mm2), and nectar glands(8/mm2); and an inner surface with large (784-1000 µ)stiff hairs(4/mm2), fewer stoma (46/mm2) and larger, more complex glands(16/mm2), presumably of a digestive nature.


1998 ◽  
Vol 23 (4) ◽  
pp. 382-387 ◽  
Author(s):  
James O. Ochanda ◽  
Eva A. C. Oduor ◽  
Rachel Galun ◽  
Mabel O. Imbuga ◽  
Kosta Y. Mumcuoglu

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