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2022 ◽  
Author(s):  
Jiwon Han ◽  
Ah-Young Oh ◽  
Bo Young Kim

Abstract BackgroundThere is a paucity of clinical data about whether sugammadex forms precipitates with other medications. This laboratory experimental study was performed to determine the drugs that produce precipitate with sugammadex. MethodsSamples of 1 mL of sugammadex were prepared in transparent cylinders, to which 1 mL of test drugs (rocuronium, neostigmine, glycopyrrolate, atropine, nitroglycerin, dobutamine, dopamine, epinephrine, vasopressin, norepinephrine, phenylephrine, ephedrine, esmolol, nicardipine, labetalol) was then added. The precipitation reaction was observed by eyes and light microscope. The pH of each drugs before and after mixing with sugammadex was measured. ResultsWhite crystals were formed when sugammadex mixed with nicardipine or labetalol. ConclusionsSugammadex formed precipitate when mixed with nicardipine or labetalol. Sufficient fluid flushing is required between injections of each drug to prevent these reactions.


Author(s):  
Camilo Romero Núñez ◽  
Laura Miranda Contreras ◽  
Rafael Heredia Cardenas

Aims: Giardia duodenalis is a globally distributed zoonotic protozoan. It has a variable prevalence. This study determines the prevalence of Giardia spp. in cat faecal samples from states of the Mexican Republic. Place and Duration of Study: Was carried out in 23 of the 32 states of the Mexican Republic, from June to December 2019.  Methodology: Stool samples from 1591 client-owned cats were analysed for the detection of G. duodenalis (cysts or trophozoites). Faecal samples were analysed by direct smear techniques with and without staining (Lugol) and centrifugal floatation (faust), and were examined under a light microscope.  Results: Of the cats sampled, 56.94% were positive for G. duodenalis. Its prevalence was associated and is a risk factor in cats that live with other animals (Chi2= 21.84, p= 0.0001; OR= 1.61, p= 0.0001), with hunting habits (Chi2= 5.53 p= 0.01, OR= 1.27 p= 0.01), with access to the outside (Chi2= 53.06, p= 0.0001; OR= 2.13, p=0.0001) and with the aqueous faeces (Chi2= 12.30, p=0.03; Chi2= 1.71, p= 0.03). Factors for not presenting Giardia spp. in faeces were, not brushing the cat (OR=0.74, p= 0.006), provenance (OR= 0.42, p=0.02), and median height (OR= 0.78, p= 0.01). Age, gender, hair type, coexistence with other cats and other stool findings were not associated as risk factors for infection. Conclusion: This study demonstrated a high overall prevalence of G. duodenalis in cats in Mexico, in addition to an association of its prevalence with risk factors such as cats living with other animals, hunting habits and access to outdoors.


2021 ◽  
Vol 43 ◽  
pp. e50828
Author(s):  
Rodrigo Diana Navarro ◽  
Sonia Maria Batista Alves ◽  
Thays Nogueira Lobo Ribeiro Ribeiro

Learning about the biology of the species is essential to the success of intensive farming. This study aimed to evaluate the semen of Thai tilapia during the four seasons of the year and thereby analyze their reproductive indices. Thus, 60 breeding males of Tilapia were used and were randomly divided into four water tanks and fed with isoproteic and isocaloric feed. The experiment lasted 12 months, starting from October 2014 and ending on September 2015. Thus, it was possible to collect sperm material of animals during the four seasons, twice a month, as well as to evaluate the water quality parameters in the tanks (temperature, pH and dissolved oxygen). The semen was evaluated from a light microscope in an increase of 100 x, then was activated with water. Motility was measured subjectively in the light microscope, as well as the percentage of sperm showing progressive motility. The duration was evaluated with the addition of a timer. For analysis of the morphology of the semen, the test consisted of morphopathology observation of 100 sperm focused in various fields throughout the slide in the light microscope. Once obtained, these data were analyzed through ANOVA and Tukey test as post-hoc analysis, with the help of the software R Statistics. Water quality factors (temperature, pH and2 dissolved) were acceptable and during the 12 month period the sperm of tilapia (Oreochromis sp.) had a good ability for fertilization, seen that it performed below the average of the percentage of critical abnormalities, and quality was perceived by the parameters that also influence fertilization (motility rate, duration of motility and vigor).


2021 ◽  
Vol 188 (12) ◽  
Author(s):  
Sang-Hyun Hwang ◽  
John Jeongseok Yang ◽  
Yoon-Hee Oh ◽  
Dae-Hyun Ko ◽  
Heungsup Sung ◽  
...  

Abstract Affordable point-of-care (POC) CD4 + T lymphocyte counting techniques have been developed as alternatives to flow cytometry-based instruments caring for patients with human immunodeficiency virus (HIV)-1. However, POC CD4 enumeration technologies can be inaccurate. Here, we developed a microparticle-based visual detector of CD4 + T lymphocytes (ImmunoSpin) using microparticles conjugated with anti-CD4 antibodies, independent of microfluidic or fluorescence detection systems. Visual enumeration of CD4 + T cells under conventional light microscope was accurate compared to flow cytometry. Microparticle-tagged CD4 + T cells were well-recognized under a light microscope. ImmunoSpin showed very good precision (coefficients of variation of ImmunoSpin were ≤ 10%) and high correlation with clinical-grade flow cytometry for the enumeration of CD4 + T cells (y = 0.4232 + 0.9485 × for the %CD4 + T cell count, R2 = 0.99). At thresholds of 200 and 350 cells/µL, there was no misclassification of the ImmunoSpin system compared to the reference flow cytometry. ImmunoSpin showed clear differential classification of CD4 + T lymphocytes from granulocytes and monocytes. Because non-fluorescence microparticle-tags and cytospin slides are used in ImmunoSpin, they can be applied to an automatic digital image analyzer. Slide preparation allows long-term storage, no analysis time limitations, and image transfer in remote areas. Graphical abstract


Author(s):  
S. Kavya ◽  
V. M. Shobini Vishali ◽  
M. Sridevi

Aims: The aim of the study is to compare and evaluate the efficacy of bronchial brush and wash cytology in diagnosing neoplastic and non- neoplastic bronchopulmonary lesions and to relate the cytological findings with the clinical diagnoses. Study Design: Retrospective descriptive study. Place and Duration of Study: Saveetha Medical College and Hospital, between January 2019 and December 2019. Methodology: Cases with both Bronchoalveolar lavage (BAL) and Bronchial brush (BB) samples (35) were included in the study. The slides were stained with Hematoxylin & Eosin stain, Papanicolaou stain, and were studied under the light microscope. The clinical points of interest were obtained from the case records at the medical records division, and histopathological findings from the histopathology registers at the Department of Pathology. Results: A total of 35 cases were studied. Follow up with histopathology after a cytological diagnosis revealed that the sensitivity of diagnosing malignancy from BAL specimen and bronchial brush sample was 58% and 92% respectively. Conclusion: The study confirms that the efficacy in assessing the risk of malignancy by  respiratory cytology using a bronchial brush specimen is higher than the bronchoalveolar lavage specimen.


Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3258
Author(s):  
Evelina Juozaitytė-Ngugu ◽  
Saulius Švažas ◽  
Donatas Šneideris ◽  
Eglė Rudaitytė-Lukošienė ◽  
Dalius Butkauskas ◽  
...  

Members of the family Corvidae are ecologically flexible omnivorous birds, particularly adaptive to urban habitats, and living in proximity to humans; these birds may serve as definitive hosts (DH) for Sarcocystis spp., but research about this is lacking. In the present study, intestinal samples from 91 corvids collected in Lithuania were molecularly tested by species-specific PCR targeting the ITS1 and cox1 genes and subsequently sequenced for the presence of Sarcocystis spp. Under a light microscope, oocysts of Sarcocystis spp. were observed in 43 samples (47.3%), while molecular methods, detected Sarcocystis spp. in 77 birds (84.6%). Eleven Sarcocystis spp. (S. columbae, S. cornixi, potentially pathogenic S. halieti, S. kutkienae, S. lari, S. turdusi, S. wobeseri, S. arctica, S. lutrae, S. ovalis, and S. oviformis) were identified in the intestinal samples from six corvid species from Lithuania. Infections with multiple Sarcocystis spp. were detected in 79.2% of the infected corvid birds. Three of the identified Sarcocystis spp. use corvids as intermediate hosts (IH); therefore, corvids may serve as IH and DH of the same Sarcocystis species. Based on molecular results and on corvid diet, omnivorous corvids may play an important role in transmitting Sarcocystis spp.


Author(s):  
Syna Pervaiz Singha ◽  
Abroo Fatima Qazi ◽  
Amir Derick Isaac

Aims: The consumption of oral form of smokeless tobacco has increased rapidly. Its use is associated with increased risk of chronic diseases like diabetes, myocardial infarction, liver disorders, cerebrovascular accidents and many other health issues. Use of tobacco in any form either smoked or chewed form leads to the absorption of nicotine which spontaneously moves into the bloodstream where it is circulated throughout the body system. Hence through this study an effort is being made to evaluate the effects produced by the locally available brand of smokeless tobacco on the histology and functioning of proximal convoluted tubules of the kidneys of the female Swiss albino rats. Place and Duration of Study: The study was conducted in Sindh Agricultural University, Tandojam and further lab work was carried out in Isra University Hyderabad. Methodology: 30 adult female Swiss albino rats were randomly selected. They were divided into three groups (n=10). Group A were taken as control. Group B&C comprised of rats which were given 5 %& 10% of smokeless tobacco respectively along with their chow diet. The feed and water were given ad libitum. Animals were sacrificed on 31st day and their kidneys were removed and weighed. The specimens were processed routinely for examination under light microscope. The sections were stained using H & E stains and examined under light microscope. Blood samples for analysis of creatinine and urea were collected. Results: A significant decrease in the weight of the kidneys, an increase in the levels of creatinine and urea were observed (P value = 0.001). Kidneys of both B & C groups showed edema, congestion and presence of cast cells when compared with the kidneys of the control group. Conclusion: From these observations, it can be inferred that the exposure of female Swiss albino rats to the smokeless form of Tobacco is associated with structural and functional damage of the kidneys.


2021 ◽  
Vol 914 (1) ◽  
pp. 012067
Author(s):  
Andianto ◽  
R Damayanti ◽  
L M Dewi ◽  
A Ismanto ◽  
H Oktariani

Abstract Merangin is a region in Jambi province, Indonesia, which well-known for its geodiversity. As part of geodiversity, fossil woods play an important role in reconstructing the ancient trees during geological history. Since the study on fossil wood origin Merangin Regency, Jambi, is still limited, this study was conducted to determine fossil wood identity through anatomical features observation and estimate the age of fossil wood samples through geological analysis. The anatomical characteristics were observed using a light microscope to identify the botanical identity of the discovered fossil wood samples. The description of anatomical features refered to the IAWA list of microscopic features for hardwood identification. The result showed that all fossil woods had similarities with the modern wood from the Dipterocarpaceae family, namely Dryobalanoxylon sp. (Kamper), Hopenium sp. (Merawan/Hopea), Shoreoxylon sp. (Meranti), and Cotylelobioxylon sp. (Giam/Resak). These fossil woods were found in different estimated geological age namely Late Permian/Perem age (254-252 million years old), Late Miocene age (7.24-5.33 million years old); Late Pliocene to Early Pleistocene age (3.60-2.58 million years old); and Holocene age (11,700 years old - present). Another approach by using Global Mapper 11 resulted that all the fossil woods were estimated grown in Permian age (290 - 250 million years).


2021 ◽  
Vol 58 (11) ◽  
pp. 684-696
Author(s):  
P. Krawczyk ◽  
A. Jansche ◽  
T. Bernthaler ◽  
G. Schneider

Abstract Image-based qualitative and quantitative structural analyses using high-resolution light microscopy are integral parts of the materialographic work on materials and components. Vibrations or defocusing often result in blurred image areas, especially in large-scale micrographs and at high magnifications. As the robustness of the image-processing analysis methods is highly dependent on the image grade, the image quality directly affects the quantitative structural analysis. We present a deep learning model which, when using appropriate training data, is capable of increasing the image sharpness of light microscope images. We show that a sharpness correction for blurred images can successfully be performed using deep learning, taking the examples of steels with a bainitic microstructure, non-metallic inclusions in the context of steel purity degree analyses, aluminumsilicon cast alloys, sintered magnets, and lithium-ion batteries. We furthermore examine whether geometric accuracy is ensured in the artificially resharpened images.


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