Improved ammonium tolerance and hydrogen production in nifA mutant strains of Rhodopseudomonas palustris

2016 ◽  
Vol 41 (48) ◽  
pp. 22824-22830 ◽  
Author(s):  
Xiao-Min Wu ◽  
Ling-Yun Zhu ◽  
Lv-Yun Zhu ◽  
Lei Wu
Keyword(s):  
Hydrogen Production ◽  
Rhodopseudomonas Palustris ◽  
Mutant Strains ◽  
Ammonium Tolerance

scholarly journals Redirection of Metabolism for Biological Hydrogen Production

2007 ◽  
Vol 73 (5) ◽  
pp. 1665-1671 ◽  
Author(s):  
Federico E. Rey ◽  
Erin K. Heiniger ◽  
Caroline S. Harwood
Keyword(s):  
Nitrogen Fixation ◽  
Hydrogen Production ◽  
Photosynthetic Bacteria ◽  
Rhodopseudomonas Palustris ◽  
Photosynthetic Bacterium ◽  
Selection Strategy ◽  
Wild Type ◽  
Purple Photosynthetic Bacteria ◽  
Mutant Strains ◽  
Different Strains

ABSTRACT A major route for hydrogen production by purple photosynthetic bacteria is biological nitrogen fixation. Nitrogenases reduce atmospheric nitrogen to ammonia with the concomitant obligate production of molecular hydrogen. However, hydrogen production in the context of nitrogen fixation is a rather inefficient process because about 75% of the reductant consumed by the nitrogenase is used to generate ammonia. In this study we describe a selection strategy to isolate strains of purple photosynthetic bacteria in which hydrogen production is necessary for growth and independent of nitrogen fixation. We obtained four mutant strains of the photosynthetic bacterium Rhodopseudomonas palustris that produce hydrogen constitutively, even in the presence of ammonium, a condition where wild-type cells do not accumulate detectable amounts of hydrogen. Some of these strains produced up to five times more hydrogen than did wild-type cells growing under nitrogen-fixing conditions. Transcriptome analyses of the hydrogen-producing mutant strains revealed that in addition to the nitrogenase genes, 18 other genes are potentially required to produce hydrogen. The mutations that caused constitutive hydrogen production mapped to four different sites in the NifA transcriptional regulator in the four different strains. The strategy presented here can be applied to the large number of diverse species of anoxygenic photosynthetic bacteria that are known to exist in nature to identify strains for which there are fitness incentives to produce hydrogen.

Thermodynamic evaluation of a photobioreactor for hydrogen production from syngas via a locally isolated Rhodopseudomonas palustris PT

2015 ◽  
Vol 40 (41) ◽  
pp. 14246-14256 ◽  
Author(s):  
Seyed Sina Hosseini ◽  
Mortaza Aghbashlo ◽  
Meisam Tabatabaei ◽  
Ghasem Najafpour ◽  
Habibollah Younesi
Keyword(s):  
Hydrogen Production ◽  
Rhodopseudomonas Palustris ◽  
Thermodynamic Evaluation

Enhanced hydrogen production by Rhodopseudomonas palustris CQK 01 with ultra-sonication pretreatment in batch culture

2011 ◽  
Vol 102 (18) ◽  
pp. 8696-8699 ◽  
Author(s):  
Xun Zhu ◽  
Xuewang Xie ◽  
Qiang Liao ◽  
Yongzhong Wang ◽  
Duujong Lee
Keyword(s):  
Hydrogen Production ◽  
Batch Culture ◽  
Rhodopseudomonas Palustris

Bioconversion characteristics of Rhodopseudomonas palustris CQK 01 entrapped in a photobioreactor for hydrogen production

2013 ◽  
Vol 135 ◽  
pp. 331-338 ◽  
Author(s):  
Yong-Zhong Wang ◽  
Qiang Liao ◽  
Xun Zhu ◽  
Rong Chen ◽  
Cheng-Long Guo ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Rhodopseudomonas Palustris

Syntrophic metabolism of a co-culture containing Clostridium cellulolyticum and Rhodopseudomonas palustris for hydrogen production

2012 ◽  
Vol 37 (16) ◽  
pp. 11719-11726 ◽  
Author(s):  
Yongqin Jiao ◽  
Ali Navid ◽  
Benjamin J. Stewart ◽  
James B. McKinlay ◽  
Michael P. Thelen ◽  
...  
Keyword(s):  
Hydrogen Production ◽  
Rhodopseudomonas Palustris ◽  
Clostridium Cellulolyticum
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