scholarly journals Comparative studies on osmosis based encapsulation of sodium diclofenac in porcine and outdated human erythrocyte ghosts

2016 ◽  
Vol 240 ◽  
pp. 14-22 ◽  
Author(s):  
Katarina Bukara ◽  
Ivana Drvenica ◽  
Vesna Ilić ◽  
Ana Stančić ◽  
Danijela Mišić ◽  
...  
Keyword(s):  
Human Erythrocyte ◽  
Comparative Studies ◽  
Erythrocyte Ghosts ◽  
Sodium Diclofenac

Lead and calcium transport in human erythrocyte

1999 ◽  
Vol 18 (5) ◽  
pp. 327-332 ◽  
Author(s):  
J V Calderón-Salinas ◽  
M A Quintanar-Escorcia ◽  
M T González-Martínez ◽  
C E Hernández-Luna
Keyword(s):  
Transport System ◽  
Human Erythrocyte ◽  
Calcium Transport ◽  
Human Erythrocytes ◽  
The Other ◽  
Erythrocyte Ghosts ◽  
Passive Transport ◽  
Ca Uptake

In this paper we report the lead (Pb) and calcium (Ca) uptake by erythrocyte ghosts. In both cases the transport was carried out by a passive transport system with two kinetic components (Michaelis-Menten and Hill). Pb and Ca were capable of inhibiting the transport of the other metal in a non-competitive way. Under hyperpolarization, the uptakes of Ca and Pb were enhanced and the Michaelis-Menten component prevailed. Both Ca and Pb uptakes were inhibited by N-ethyl-maleimide to the same extent. These results indicate that Pb and Ca share the same permeability pathway in human erythrocytes and that this transport system is electrogenic.

The perturbation of the human erythrocyte membrane by phospholipase C

1975 ◽  
Vol 19 (2) ◽  
pp. 341-355
Author(s):  
A.R. Limbrick ◽  
S. Knutton
Keyword(s):  
Phospholipase C ◽  
Surface Area ◽  
Human Erythrocyte ◽  
Lipid Droplets ◽  
External Surface ◽  
Particle Density ◽  
Freeze Fracture ◽  
Erythrocyte Ghosts ◽  
Human Erythrocyte Membrane ◽  
Small Areas

A study has been made of freeze-fractured preparations of erythrocyte ghosts modified by phospholipase C (Clostridium welchii). Such membranes show a decrease in surface area of up to about 47% and lipid droplets appear on their external surface but there is no loss of protein. Freeze-fracture of maximally hydrolysed membranes exposes only very small areas of A faces and these appear particle-free. Most of the membranes are simply cross-fractured. At lower levels of hydrolysis there is more extensive exposure of A fracture faces but the particle density is less than in control preparations. If such exposed faces were representative of the whole membrane then the particle density would have been expected to increase. It is suggested either that areas of membrane with increased particle density do not fracture or that the particles revealed by freeze-fracture involve phospholipid as well as protein and are not revealed in the absence of phospholipid.

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