Sugarcane biomass as а potential carrier for drug delivery system

Non-steroidal anti-inflammatory drugs are well-known medications for reducing pain and a group of drugs that can cause mucosal damage of the stomach. The negative effects on the digestive system can be reduced by immobilization of drugs on various carriers, for instance, on the components of plant biomass, for the creation of drug delivery system. Plant biomass is a lignocellulosic complex consisting of lignin, cellulose and hemicellulose that can potentially be regarded as a carrier of pharmaceuticals. Sugarcane residues such as bagasse and straw are biomass by-products of the sugarcane industry. One of the prospective ways for their efficient utilization can include chemical processing with the aim of obtaining effective biosorbents or so-called carriers of different composition and structure. The aim of the work was to study the structural, morphological, and sorption properties of cellulose, lignin, and lignocellulose, derived from sugarcane biomass (bagasse and straw) by means of delignification and hydrolysis, as potential components for drug delivery system. Sugarcane straw samples show higher densities in comparison with bagasse samples. Both lignin samples from bagasse and straw have greater bulk and true density if compared to other materials from sugarcane biomass of cellulosic and lignocellulosic nature. The increase in adsorption pore volume in lignins is observed, being indicative of better sorption ability. Both samples of cellulose and lignocellulose from straw have greater pore structure if compared to the initial material. The values of sodium diclofenac sorption efficiency correlate with the values of pore volume for corresponding materials. Lignin from sugarcane straw, which shows greater porosity, has greater sorption properties. SEM images show that the initial materials and treated materials have complex morphology. FTIR spectra show a clear difference in the structure of lignocellulose, cellulose, and lignin from sugarcane bagasse and straw. The potential application of biopolymers from bagasse and straw as organic carriers of sodium diclofenac was studied. With this purpose, plant polymers were impregnated with an alcoholic solution of sodium diclofenac and the desorption process was investigated. The lignin sample from sugarcane straw has a longer period of drug release, which indicates the obtained effect of prolongation.

Electrochemical Method for Ease Determination of Sodium Diclofenac Trace Levels in Water Using Graphene—Multi-Walled Carbon Nanotubes Paste Electrode

Sodium diclofenac (DCF) presence reported in water use cycle at various concentrations including trace levels necessitates continuous development of advanced analytical method for its determination. In this work, ease electrochemical methods for DCF determination based on voltammetric and amperometric techniques were proposed using a simple combination of graphene with multi-walled carbon nanotubes as paste electrode. Integration of the graphene with multi-walled carbon nanotubes enlarged the electroactive surface area of the electrode and implicitly enhanced the electrochemical response for DCF determination. On the basis of the sorption autocatalytic effect manifested at low concentration of DCF, we found that the preconcentration step applied prior to differential-pulsed voltammetry (DPV) and multiple-pulsed amperometry (MPA) allowed for the enhancement of the electroanalytical performance of the DCF electrochemical detections, which were validated by testing in tap water. The lowest limit of detection (LOD) of 1.40 ng·L−1 was found using preconcentration prior to DPV under optimized operating conditions, which is better than that reached by other carbon-based electrodes reported in the literature.

Polycaprolactone Composite Micro/Nanofibrous Material as an Alternative to Restricted Access Media for Direct Extraction and Separation of Non-Steroidal Anti-Inflammatory Drugs from Human Serum Using Column-Switching Chromatography

Application of the poly-ɛ-caprolactone composite sorbent consisting of the micro- and nanometer fibers for the on-line extraction of non-steroidal anti-inflammatory drugs from a biological matrix has been introduced. A 100 μL human serum sample spiked with ketoprofen, naproxen, sodium diclofenac, and indomethacin was directly injected in the extraction cartridge filled with the poly-ɛ-caprolactone composite sorbent. This cartridge was coupled with a chromatographic instrument via a six-port switching valve allowing the analyte extraction and separation within a single analytical run. The 1.5 min long extraction step isolated the analytes from the proteinaceous matrix was followed by their 13 min HPLC separation using Ascentis Express RP-Amide (100 × 4.6 mm, 5 µm) column. The recovery of all analytes from human serum tested at three concentration levels ranged from 70.1% to 118.7%. The matrix calibrations were carried out in the range 50 to 20,000 ng mL−1 with correlation coefficients exceeding 0.996. The detection limit was 15 ng mL−1, and the limit of quantification corresponded to 50 ng mL−1. The developed method was validated and successfully applied for the sodium diclofenac determination in real patient serum. Our study confirmed the ability of the poly-ɛ-caprolactone composite sorbent to remove the proteins from the biological matrix, thus serving as an alternative to the application of restricted-access media.

SOLID DISPERSION FOR INCREASING DISSOLUTION RATE OF SODIUM DICLOFENAC WITH VARIATIONS OF POLYVINYL PYRROLIDONE K30

Diclofenac sodium is included in class II category based on biopharmaceutics classification system (BCS), sodium diclofenac has low solubility and high permeability. Low solubility will affect absorption of drugs in body because rate of dissolution will decrease. PVP K30 is inert carrier that dissolves easily in water and can affect solubility of an active drug substance. To know solid dispersion system increasing dissolution rate of sodium diclofenac by adding variations concentration of PVP K30. Solid dispersion uses solvent method with variations concentration of PVP K30 1:3, 1:5, 1:7 and 1:9. Test physical properties of solid dispersions using a moisture test and compressibility. Solid dispersion dissolution test using type 2 dissolutions test and determination of concentration using UV-VIS spectrophotometry. Test results were analyzed using One Way ANOVA and continued test. Solid dispersion has a good physical whit moisture percentage not >5% and compressibility not >20%. Solid dispersion of sodium diclofenac with addition of PVP K30 can increase dissolution rate compared to pure sodium diclofenac (p<0,05) with highest at ratio 1:7. Each comparison has significant difference (p<0,05) expect in ratio 1:9. Solid dispersion of sodium diclofenac with PVP K30 can increase dissolution rate of pure sodium diclofenac.