Differentiation of induced pluripotent stem cells into definitive endoderm on Activin A-functionalized gradient surfaces

2021 ◽  
Vol 325 ◽  
pp. 173-178 ◽  
Author(s):  
Linnea Andreasson ◽  
Hanne Evenbratt ◽  
Reza Mobini ◽  
Stina Simonsson
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Activin A ◽  
Definitive Endoderm ◽  
Gradient Surfaces ◽  
Induced Pluripotent

scholarly journals Activin A maintains pluripotency markers and proliferative potential of human induced pluripotent stem cells

2011 ◽  
Vol 2 (3) ◽  
pp. 405-408 ◽  
Author(s):  
MINORU TOMIZAWA ◽  
FUMINOBU SHINOZAKI ◽  
TAKAO SUGIYAMA ◽  
SHIGENORI YAMAMOTO ◽  
MAKOTO SUEISHI ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Activin A ◽  
Proliferative Potential ◽  
Pluripotency Markers ◽  
Induced Pluripotent

scholarly journals Definitive Endoderm Formation from Plucked Human Hair-Derived Induced Pluripotent Stem Cells and SK Channel Regulation

2013 ◽  
Vol 2013 ◽  
pp. 1-13 ◽  
Author(s):  
Anett Illing ◽  
Marianne Stockmann ◽  
Narasimha Swamy Telugu ◽  
Leonhard Linta ◽  
Ronan Russell ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Induced Pluripotent Stem Cells ◽  
Cell Fate ◽  
Pluripotent Stem Cells ◽  
Human Hair ◽  
Definitive Endoderm ◽  
Sk Channel ◽  
Induced Pluripotent

Pluripotent stem cells present an extraordinary powerful tool to investigate embryonic development in humans. Essentially, they provide a unique platform for dissecting the distinct mechanisms underlying pluripotency and subsequent lineage commitment. Modest information currently exists about the expression and the role of ion channels during human embryogenesis, organ development, and cell fate determination. Of note, small and intermediate conductance, calcium-activated potassium channels have been reported to modify stem cell behaviour and differentiation. These channels are broadly expressed throughout human tissues and are involved in various cellular processes, such as the after-hyperpolarization in excitable cells, and also in differentiation processes. To this end, human induced pluripotent stem cells (hiPSCs) generated from plucked human hair keratinocytes have been exploitedin vitroto recapitulate endoderm formation and, concomitantly, used to map the expression of the SK channel (SKCa) subtypes over time. Thus, we report the successful generation of definitive endoderm from hiPSCs of ectodermal origin using a highly reproducible and robust differentiation system. Furthermore, we provide the first evidence that SKCas subtypes are dynamically regulated in the transition from a pluripotent stem cell to a more lineage restricted, endodermal progeny.

Small Molecules Differentiate Definitive Endoderm from Human Induced Pluripotent Stem Cells on PCL Scaffold

2014 ◽  
Vol 173 (7) ◽  
pp. 1727-1736 ◽  
Author(s):  
Elham Hoveizi ◽  
Sirus Khodadadi ◽  
Shima Tavakol ◽  
Oveis Karima ◽  
Mohammad Ali Nasiri-Khalili
Keyword(s):  
Stem Cells ◽  
Small Molecules ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Definitive Endoderm ◽  
Induced Pluripotent

Activin A is essential for Feeder-free culture of human induced pluripotent stem cells

2013 ◽  
Vol 114 (3) ◽  
pp. 584-588 ◽  
Author(s):  
Minoru Tomizawa ◽  
Fuminobu Shinozaki ◽  
Takao Sugiyama ◽  
Shigenori Yamamoto ◽  
Makoto Sueishi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Activin A ◽  
Induced Pluripotent
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