A sensitive and robust HPLC method to quantify recombinant antibody fragments in E . coli crude cell lysate

2018 ◽  
Vol 1083 ◽  
pp. 242-248 ◽  
Author(s):  
Thomas Gundinger ◽  
Alexander Pansy ◽  
Oliver Spadiut
Keyword(s):  
Recombinant Antibody ◽  
Hplc Method ◽  
Antibody Fragments ◽  
Crude Cell Lysate ◽  
E Coli ◽  
Cell Lysate ◽  
Recombinant Antibody Fragments

scholarly journals High-yield expression of periplasmic single-chain variable fragments by solid Escherichia coli cultures

BioTechniques ◽  
2021 ◽  
Author(s):  
Yoshiro Hanyu ◽  
Mieko Kato
Keyword(s):  
Escherichia Coli ◽  
Liquid Culture ◽  
Recombinant Antibody ◽  
Antibody Fragments ◽  
High Yield ◽  
Single Chain ◽  
E Coli ◽  
Recombinant Antibody Fragments ◽  
Single Chain Variable Fragments ◽  
High Yields

High-yield expression of quality antibody fragments is indispensable for research and diagnosis. Most recombinant antibody fragments are expressed in Escherichia coli using liquid cultures; however, their yields and quality are often poor. Here the authors expressed a single-chain variable fragment in E. coli cultivated on the wet surface of a solid support. Compared with a liquid culture, the authors obtained 2.5-times more single-chain variable fragments with membrane-cultivated E. coli. This method has two important advantages: it enables high yields of periplasmic single-chain variable fragments compared with liquid culture and offers simple and rapid expression and extraction.

The venom of the scorpion Centruroides limpidus, which causes the highest number of stings in Mexico, is neutralized by two recombinant antibody fragments

2021 ◽  
Vol 137 ◽  
pp. 247-255
Author(s):  
Guillermo Fernández-Taboada ◽  
Lidia Riaño-Umbarila ◽  
Alejandro Olvera-Rodríguez ◽  
Ilse Viridiana Gómez-Ramírez ◽  
Luis Fernando Losoya-Uribe ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Recombinant Antibody Fragments

Isolation and characterisation of Ebolavirus-specific recombinant antibody fragments from murine and shark immune libraries

2011 ◽  
Vol 48 (15-16) ◽  
pp. 2027-2037 ◽  
Author(s):  
Sarah A. Goodchild ◽  
Helen Dooley ◽  
Randal J. Schoepp ◽  
Martin Flajnik ◽  
Stephen G. Lonsdale
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
Recombinant Antibody Fragments

scholarly journals High-Affinity Recombinant Antibody Fragments (Fabs) Can Be Applied in Peptide Enrichment Immuno-MRM Assays

2014 ◽  
Vol 13 (4) ◽  
pp. 2187-2196 ◽  
Author(s):  
Jeffrey R. Whiteaker ◽  
Lei Zhao ◽  
Christian Frisch ◽  
Francisco Ylera ◽  
Stefan Harth ◽  
...  
Keyword(s):  
Recombinant Antibody ◽  
Antibody Fragments ◽  
High Affinity ◽  
Recombinant Antibody Fragments ◽  
Peptide Enrichment
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