Rapid detection method of carbapenemase-producing Enterobacteriaceae by MALDI-TOF MS with imipenem/cilastatin (KB) disc and zinc sulfate solution

Author(s):  
Megumi Oho ◽  
Yumiko Funashima ◽  
Zenzo Nagasawa ◽  
Hiroshi Miyamoto ◽  
Eisaburo Sueoka
2019 ◽  
Vol 74 (5) ◽  
pp. 1269-1276 ◽  
Author(s):  
Saikat Paul ◽  
Shreya Singh ◽  
Arunaloke Chakrabarti ◽  
Shivaprakash M Rudramurthy ◽  
Anup K Ghosh

2014 ◽  
Vol 105 ◽  
pp. 98-101 ◽  
Author(s):  
Y. Hoyos-Mallecot ◽  
C. Riazzo ◽  
C. Miranda-Casas ◽  
M.D. Rojo-Martín ◽  
J. Gutiérrez-Fernández ◽  
...  

2017 ◽  
Vol 139 ◽  
pp. 138-142 ◽  
Author(s):  
Tomás García-Cayuela ◽  
Teresa Requena ◽  
M. Carmen Martínez-Cuesta ◽  
Carmen Peláez

2011 ◽  
Vol 23 (6) ◽  
pp. 1202-1207 ◽  
Author(s):  
Hiroyuki Satoh ◽  
Toyofumi Yamauchi ◽  
Masahiro Yamasaki ◽  
Yoshimitsu Maede ◽  
Akira Yabuki ◽  
...  

The concentration of GM1 (monosialotetrahexosyl ganglioside) in cerebrospinal fluid (CSF) is markedly increased in dogs with GM1 gangliosidosis due to GM1 accumulation in the central nervous system and leakage to the CSF. The present study established a rapid and simple method for detection of accumulated GM1 in the CSF in dogs with GM1 gangliosidosis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI TOF MS) and discusses the usefulness of this method for the rapid diagnosis and/or high-risk screening of this disease in domestic animals. Cerebrospinal fluid was collected from normal dogs and 4- to 11-month-old Shiba dogs with GM1 gangliosidosis. The MALDI TOF MS analysis was carried out in combination with a special sample plate and a simple desalting step on the plate. Specific signs of GM1 could be detected in the standard GM1 solutions at concentrations of 50 nmol/l or more. The signs were also clearly detected in CSF (131–618 nmol/l) in affected dogs, but not in normal canine CSF (12 ± 5 nmol/l, mean ± standard deviation). The results demonstrated that MALDI TOF MS can detect GM1 accumulated in canine CSF even in the early stage of the disease. In conclusion, the rapid detection of increased CSF GM1 using MALDI TOF MS is a useful method for diagnosis and/or screening for canine GM1 gangliosidosis.


2021 ◽  
pp. 131057
Author(s):  
Anastasia S. Kritikou ◽  
Reza Aalizadeh ◽  
Dimitrios E. Damalas ◽  
Ioanna V. Barla ◽  
Carsten Baessmann ◽  
...  

2017 ◽  
Vol 56 (2) ◽  
pp. 234-241 ◽  
Author(s):  
Saikat Paul ◽  
Pankaj Singh ◽  
Shamanth A S ◽  
Shivaprakash M Rudramurthy ◽  
Arunaloke Chakrabarti ◽  
...  

2013 ◽  
Vol 7 (11-12) ◽  
pp. 767-778 ◽  
Author(s):  
Markus Kostrzewa ◽  
Katrin Sparbier ◽  
Thomas Maier ◽  
Sören Schubert

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