scholarly journals Functional recombinant human complement C1q with different affinity tags

2021 ◽  
Vol 492 ◽  
pp. 113001
Author(s):  
Isabelle Bally ◽  
Sarah Ancelet ◽  
Jean-Baptiste Reiser ◽  
Véronique Rossi ◽  
Christine Gaboriaud ◽  
...  
Keyword(s):  
Human Complement ◽  
Affinity Tags ◽  
Complement C1q

scholarly journals Trichinella spiralis Calreticulin S-Domain Binds to Human Complement C1q to Interfere With C1q-Mediated Immune Functions

2020 ◽  
Vol 11 ◽  
Author(s):  
Shuai Shao ◽  
Chunyue Hao ◽  
Bin Zhan ◽  
Qinghui Zhuang ◽  
Limei Zhao ◽  
...  
Keyword(s):  
Trichinella Spiralis ◽  
Human Complement ◽  
Immune Functions ◽  
Complement C1q

scholarly journals Trichinella spiralis Calreticulin Binds Human Complement C1q As an Immune Evasion Strategy

2017 ◽  
Vol 8 ◽  
Author(s):  
Limei Zhao ◽  
Shuai Shao ◽  
Yi Chen ◽  
Ximeng Sun ◽  
Ran Sun ◽  
...  
Keyword(s):  
Immune Evasion ◽  
Trichinella Spiralis ◽  
Human Complement ◽  
Evasion Strategy ◽  
Complement C1q

scholarly journals Expression of recombinant human complement C1q allows identification of the C1r/C1s-binding sites

2013 ◽  
Vol 110 (21) ◽  
pp. 8650-8655 ◽  
Author(s):  
I. Bally ◽  
S. Ancelet ◽  
C. Moriscot ◽  
F. Gonnet ◽  
A. Mantovani ◽  
...  
Keyword(s):  
Binding Sites ◽  
Human Complement ◽  
Complement C1q

The binding porperties of human complement C1q; Interaction with mucopolysaccharides

1982 ◽  
Vol 19 (11) ◽  
pp. 1361 ◽  
Author(s):  
D.H. Bing ◽  
S. Almeda ◽  
R.D. Rosenberg
Keyword(s):  
Human Complement ◽  
Complement C1q

scholarly journals Trichinella spiralis Paramyosin Binds Human Complement C1q and Inhibits Classical Complement Activation

2015 ◽  
Vol 9 (12) ◽  
pp. e0004310 ◽  
Author(s):  
Ran Sun ◽  
Xi Zhao ◽  
Zixia Wang ◽  
Jing Yang ◽  
Limei Zhao ◽  
...  
Keyword(s):  
Complement Activation ◽  
Trichinella Spiralis ◽  
Human Complement ◽  
Complement C1q ◽  
Classical Complement

scholarly journals Gonococcal infection in a nonhuman host is determined by human complement C1q.

1995 ◽  
Vol 63 (12) ◽  
pp. 4790-4794 ◽  
Author(s):  
S Nowicki ◽  
M G Martens ◽  
B J Nowicki
Keyword(s):  
Human Complement ◽  
Gonococcal Infection ◽  
Complement C1q

Affinity Tags for Protein Purification

2020 ◽  
Vol 21 (8) ◽  
pp. 821-830
Author(s):  
Vibhor Mishra
Keyword(s):  
Protein Purification ◽  
Protein Complexes ◽  
Affinity Purification ◽  
Protein Domain ◽  
Affinity Tag ◽  
Small Peptides ◽  
Affinity Tags ◽  
C Terminus ◽  
Solubility Enhancers ◽  
As Solubility

The affinity tags are unique proteins/peptides that are attached at the N- or C-terminus of the recombinant proteins. These tags help in protein purification. Additionally, some affinity tags also serve a dual purpose as solubility enhancers for challenging protein targets. By applying a combinatorial approach, carefully chosen affinity tags designed in tandem have proven to be very successful in the purification of single proteins or multi-protein complexes. In this mini-review, the key features of the most commonly used affinity tags are discussed. The affinity tags have been classified into two significant categories, epitope tags, and protein/domain tags. The epitope tags are generally small peptides with high affinity towards a chromatography resin. The protein/domain tags often perform double duty as solubility enhancers as well as aid in affinity purification. Finally, protease-based affinity tag removal strategies after purification are discussed.

scholarly journals The Crystal Structure of C2a, the Catalytic Fragment of Classical Pathway C3 and C5 Convertase of Human Complement

2007 ◽  
Vol 367 (1) ◽  
pp. 224-233 ◽  
Author(s):  
Vengadesan Krishnan ◽  
Yuanyuan Xu ◽  
Kevin Macon ◽  
John E. Volanakis ◽  
Sthanam V.L. Narayana
Keyword(s):  
Crystal Structure ◽  
Classical Pathway ◽  
Human Complement ◽  
Catalytic Fragment
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