Establishment of a homogeneous immunoassay-light-initiated chemiluminescence assay for detecting anti-Müllerian hormone in human serum

2021 ◽  
Vol 494 ◽  
pp. 113059
Author(s):  
Yang Yu ◽  
Tiantian She ◽  
Liang Huang ◽  
Jingxin Xu ◽  
Juanjuan Yan ◽  
...  
Keyword(s):  
Human Serum ◽  
Homogeneous Immunoassay ◽  
Chemiluminescence Assay

CHEMILUMINESCENCE ASSAY FOR LIPASE ACTIVITY IN HUMAN SERUM BY USING A PROENHANCER SUBSTRATE

2005 ◽  
Author(s):  
T ICHIBANGASE ◽  
C HAMABE ◽  
Y OHBA ◽  
N KISHIKAWA ◽  
K NAKASHIMA ◽  
...  
Keyword(s):  
Human Serum ◽  
Lipase Activity ◽  
Chemiluminescence Assay

Stable liposomes for assays of human sera

1993 ◽  
Vol 39 (7) ◽  
pp. 1439-1443 ◽  
Author(s):  
Y Ishimori ◽  
K Rokugawa
Keyword(s):  
Human Serum ◽  
Functional Groups ◽  
Protein Antigens ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Homogeneous Immunoassay ◽  
Sandwich Type ◽  
Human Sera

Abstract We report a novel homogeneous immunoassay system involving protein-bearing liposome-encapsulated carboxyfluorescein as a release marker. We applied this system to determine protein antigens, e.g., ferritin, in human serum samples by a sandwich-type assay. Liposomal lysis was observed in many samples, even though no second antibody was added to the reaction mixture. We demonstrated that the functional groups used to immobilize an antibody on liposomes are related to this phenomenon. Stable liposomes in human sera were prepared by incorporating bromoacetyl groups instead of the dithiopyridyl groups used previously. A good correlation (y = 0.98x - 8.81, r = 0.98, Sx/y = 66.9, range approximately 10-2000 micrograms/L) with results by RIA was obtained in the ferritin measurement of 53 patients' sera by using these liposomes.

Column chromatography and immunoassay compared for measuring the isoenzymes of aspartate aminotransferase in serum.

1979 ◽  
Vol 25 (10) ◽  
pp. 1691-1695 ◽  
Author(s):  
E J Sampson ◽  
W H Hannon ◽  
S S McKneally ◽  
C McKenzie ◽  
S A Miller ◽  
...  
Keyword(s):  
Human Serum ◽  
Least Squares ◽  
Column Chromatography ◽  
Standard Error ◽  
Aspartate Aminotransferase ◽  
Chromatographic Method ◽  
Homogeneous Immunoassay ◽  
Analytical Recovery ◽  
Column Chromatographic

Abstract We compare a column-chromatographic method and a homogeneous immunoassay method for separately measuring the mitochondrial and cytoplasmic isoenzymes of aspartate aminotransferase. Analytical recovery for the two methods averaged 102% (SD, 2%) and 103% (SD, 4%), respectively, for 11 pools prepared by adding the purified isoenzymes to serum and 102% (SD 8.9%) and 89% (SD, 8.1%) for 26 unaltered specimens of human serum. In comparing the results of the immunoassay method (y) to the chromatographic method (x), our measurements agreed closely for the mitochondrial (y = 0.947 X + 7, r = 0.9991, standard error of estimate = 2.9 U/L) and cytoplasmic (y = 0.92x-6, r = 0.9995, standard error of estimate = 2.1 U/L) isoenzymes in pools prepared from the purified isoenzymes. Similar measurements of the 26 human serum specimens yielded the following results for least-squares evaluation; cytoplasmic isoenzyme y = 1.03x-11, r = 0.994, and standard error of estimate = 6.0 U/L; mitochondrial isoenzyme y = 0.75x+0, r = 0.927, and standard error of estimate = 3.9 U/L.

scholarly journals Development of a Sol Particle Homogeneous Immunoassay for Measuring Full‐Length Selenoprotein P in Human Serum

2014 ◽  
Vol 30 (2) ◽  
pp. 114-122 ◽  
Author(s):  
Mutsumi Tanaka ◽  
Yoshiro Saito ◽  
Hirofumi Misu ◽  
Seiji Kato ◽  
Yuki Kita ◽  
...  
Keyword(s):  
Human Serum ◽  
Full Length ◽  
Selenoprotein P ◽  
Homogeneous Immunoassay
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