scholarly journals Development of a multiplex Luminex assay for detecting swine antibodies to structural and nonstructural proteins of foot-and-mouth disease virus in Taiwan

2016 ◽  
Vol 49 (2) ◽  
pp. 196-207 ◽  
Author(s):  
Tsu-Han Chen ◽  
Fan Lee ◽  
Yeou-Liang Lin ◽  
Chu-Hsiang Pan ◽  
Chia-Ni Shih ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Nonstructural Proteins ◽  
Luminex Assay ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Development of a Luminex assay for the detection of swine antibodies to non-structural proteins of foot-and-mouth disease virus

2013 ◽  
Vol 396 (1-2) ◽  
pp. 87-95 ◽  
Author(s):  
Tsu-Han Chen ◽  
Fan Lee ◽  
Yeou-Liang Lin ◽  
Chu-Hsiang Pan ◽  
Chia-Ni Shih ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Structural Proteins ◽  
Luminex Assay ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Mutagenesis Mapping of RNA Structures within the Foot-and-Mouth Disease Virus Genome Reveals Functional Elements Localized in the Polymerase (3D pol )-Encoding Region

mSphere ◽  
2021 ◽  
Author(s):  
Lidia Lasecka-Dykes ◽  
Fiona Tulloch ◽  
Peter Simmonds ◽  
Garry A. Luke ◽  
Paolo Ribeca ◽  
...  
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Virus Genome ◽  
Mouth Disease ◽  
Rna Structures ◽  
Nonstructural Proteins ◽  
Functional Elements ◽  
Subgenomic Replicon ◽  
Mouth Disease Virus ◽  
Foot And Mouth

Some RNA structures formed by the genomes of RNA viruses are critical for viral replication. Our study shows that of 46 conserved RNA structures located within the regions of the foot-and-mouth disease virus (FMDV) genome that encode the nonstructural proteins, only 3 are essential for replication of an FMDV subgenomic replicon.

scholarly journals Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

2020 ◽  
Vol 32 (6) ◽  
pp. 933-937
Author(s):  
Clare F. J. Browning ◽  
Antonello Di Nardo ◽  
Lissie Henry ◽  
Tim Pollard ◽  
Lynne Hendry ◽  
...  
Keyword(s):  
Disease Virus ◽  
Disease Surveillance ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Screen Test ◽  
Mouth Disease ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Thermo Fisher Scientific

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

scholarly journals Identification of T-Cell Epitopes in Nonstructural Proteins of Foot-and-Mouth Disease Virus

2001 ◽  
Vol 75 (7) ◽  
pp. 3164-3174 ◽  
Author(s):  
E. Blanco ◽  
M. Garcia-Briones ◽  
A. Sanz-Parra ◽  
P. Gomes ◽  
E. De Oliveira ◽  
...  
Keyword(s):  
T Cell ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
T Cell Epitopes ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Bovine Serum Panel for Evaluating Foot-and-Mouth Disease Virus Nonstructural Protein Antibody Tests

2007 ◽  
Vol 19 (5) ◽  
pp. 539-544 ◽  
Author(s):  
Satya Parida ◽  
Lucy Fleming ◽  
Debi Gibson ◽  
Pip A. Hamblin ◽  
Santina Grazioli ◽  
...  
Keyword(s):  
Disease Virus ◽  
Nonstructural Protein ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Infected Cattle ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Contact Exposure ◽  
Antibody Tests

A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculation or contact exposure with 4 serotypes of foot-and-mouth disease virus (FMDV) with or without prior vaccination. Virus replication and persistence had been characterized in all of the animals. The proportion of the sera scored positive by 5 tests for antibodies to the nonstructural proteins of FMDV varied, suggesting that the panel can discriminate between the sensitivity with which such tests are able to identify infected cattle. Use of this panel will help in assessment of new tests and quality control of existing methods.

Multiplexed Detection of Antibodies to Nonstructural Proteins of Foot-and-Mouth Disease Virus

2006 ◽  
Vol 78 (15) ◽  
pp. 5462-5468 ◽  
Author(s):  
Julie Perkins ◽  
Alfonso Clavijo ◽  
Benjamin J. Hindson ◽  
Raymond J. Lenhoff ◽  
Mary T. McBride
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Nonstructural Proteins ◽  
Multiplexed Detection ◽  
Mouth Disease Virus ◽  
Foot And Mouth

scholarly journals Shifts in the Selection-Drift Balance Drive the Evolution and Epidemiology of Foot-and-Mouth Disease Virus

2008 ◽  
Vol 83 (2) ◽  
pp. 781-790 ◽  
Author(s):  
Damien C. Tully ◽  
Mario A. Fares
Keyword(s):  
Disease Virus ◽  
Protein Structures ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Nonstructural Proteins ◽  
Adaptive Mutations ◽  
Persistent Infections ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Synonymous Sites

ABSTRACT Foot-and-mouth disease virus (FMDV) is the causative agent of an acute vesicular disease affecting wild and domesticated animals. Despite the economic burden of the disease and all efforts to eradicate it, FMD outbreaks continue to emerge unexpectedly in developed and developing countries. Correlation of the mutational dynamics of the virus with its epidemiology remains unexplored. Analysis of 103 complete genomes representing the seven serotypes shows the important role that selection plays in the genomic evolution of viral isolates for serotypes. We identified selection and relaxed constraints due to genetic drift through analyses of synonymous sites. Finally, we investigated interactions between mutations that showed coevolving patterns and analyzed, based on protein structures, slightly deleterious and compensatory mutational dynamics. Specifically, we demonstrate that structurally exposed capsid proteins present a greater number of adaptive mutations and relaxed selection than nonstructural proteins. Such events have been magnified during the evolution of the southern African virus types (SATs). These shifts in selection-drift balance have generated the great antigenic and genetic diversity observed for SAT serotypes and that are responsible for epizootics on the continent of Africa. The high number of slightly deleterious and compensatory mutations in SAT serotypes in structural proteins is testament to such balance plasticity. The significant accumulation of these coevolving mutations in African serotypes supports their contribution in generating adaptive immune-escaping mutants and in establishing persistent infections. The reverse of this pattern in nonstructural proteins reveals the neutral fixation of mutations in the more widely spread and commonly studied Euro-Asiatic serotypes.

scholarly journals Serological probes for some foot-and-mouth disease virus nonstructural proteins

Virus Genes ◽  
1989 ◽  
Vol 3 (1) ◽  
pp. 29-44 ◽  
Author(s):  
Michael Tesar ◽  
Hans-Gerhard Berger ◽  
Otfried Marquardt
Keyword(s):  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Nonstructural Proteins ◽  
Mouth Disease Virus ◽  
Foot And Mouth ◽  
Serological Probes
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