Repetitive cross-polarization contacts via equilibration-re-equilibration of the proton bath: Sensitivity enhancement for NMR of membrane proteins reconstituted in magnetically aligned bicelles

2011 ◽  
Vol 212 (1) ◽  
pp. 245-248 ◽  
Author(s):  
Wenxing Tang ◽  
Alexander A. Nevzorov
Keyword(s):  
Membrane Proteins ◽  
Sensitivity Enhancement ◽  
Cross Polarization ◽  
Magnetically Aligned Bicelles ◽  
Magnetically Aligned

Uniaxial Diffusional Narrowing of NMR Lineshapes for Membrane Proteins Reconstituted in Magnetically Aligned Bicelles and Macrodiscs

2018 ◽  
Vol 49 (12) ◽  
pp. 1335-1353 ◽  
Author(s):  
Deanna M. Tesch ◽  
Zhaleh Pourmoazzen ◽  
Emmanuel O. Awosanya ◽  
Alexander A. Nevzorov
Keyword(s):  
Membrane Proteins ◽  
Magnetically Aligned Bicelles ◽  
Magnetically Aligned

Diffusion NMR and bicelle morphology

2011 ◽  
Vol 89 (9) ◽  
pp. 1021-1035 ◽  
Author(s):  
Peter M. Macdonald ◽  
Ronald Soong
Keyword(s):  
Lateral Diffusion ◽  
Diffusion Method ◽  
Nmr Studies ◽  
Diffusion Nmr ◽  
Magnetically Aligned Bicelles ◽  
Diffusion Studies ◽  
Membrane Bound ◽  
Nmr Diffusion ◽  
Pfg Nmr ◽  
Magnetically Aligned

This minireview focuses on diffusion NMR studies in bicelles. Following a discourse on diffusion fundamentals, and a comparative overview of fluorescence and NMR-based techniques for measuring diffusion, the pulsed field gradient (PFG) NMR diffusion method is introduced, emphasizing its specific advantages and limitations when applied to diffusion measurements in macroscopically oriented lamellar systems such as magnetically aligned bicelles. The utility of PFG NMR diffusion measurements in bicellar model membrane systems for examining lateral diffusion of membrane-bound molecular species is demonstrated, along with certain features of lateral diffusion that such studies illuminate. Further, those aspects of bicelle morphology that have been resolved using PFG NMR diffusion studies of various molecular weight soluble polymeric species are reviewed. The discussion concludes with an outline of future prospects for diffusion NMR studies in bicelles.

scholarly journals A bifunctional spin label reports the structural topology of phospholamban in magnetically-aligned bicelles

2016 ◽  
Vol 262 ◽  
pp. 50-56 ◽  
Author(s):  
Jesse E. McCaffrey ◽  
Zachary M. James ◽  
Bengt Svensson ◽  
Benjamin P. Binder ◽  
David D. Thomas
Keyword(s):  
Spin Label ◽  
Structural Topology ◽  
Magnetically Aligned Bicelles ◽  
Magnetically Aligned
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