scholarly journals Evaluation of cartilage repair in the distal femur after autologous chondrocyte transplantation using T2 relaxation time and dGEMRIC

2007 ◽  
Vol 15 (4) ◽  
pp. 372-378 ◽  
Author(s):  
J.E. Kurkijärvi ◽  
L. Mattila ◽  
R.O. Ojala ◽  
A.I. Vasara ◽  
J.S. Jurvelin ◽  
...  
Keyword(s):  
Relaxation Time ◽  
Cartilage Repair ◽  
Distal Femur ◽  
Autologous Chondrocyte Transplantation ◽  
T2 Relaxation Time ◽  
Chondrocyte Transplantation ◽  
T2 Relaxation ◽  
Autologous Chondrocyte

Matrix Production Affects MRI Outcomes After Matrix-Associated Autologous Chondrocyte Transplantation in the Knee

2017 ◽  
Vol 45 (10) ◽  
pp. 2238-2246 ◽  
Author(s):  
Christian Albrecht ◽  
Carla-Antonia Reuter ◽  
David Stelzeneder ◽  
Lukas Zak ◽  
Brigitte Tichy ◽  
...  
Keyword(s):  
Gene Expression ◽  
Relaxation Time ◽  
Collagen Type ◽  
Autologous Chondrocyte Transplantation ◽  
T2 Relaxation Time ◽  
Chondrocyte Transplantation ◽  
T2 Relaxation ◽  
Native Cartilage ◽  
Matrix Production ◽  
Autologous Chondrocyte

Background: Matrix-associated autologous chondrocyte transplantation (MACT) has been an effective therapy for large, full-thickness cartilage lesions for years. However, little is known about how graft maturation is affected by characteristics of transplanted chondrocytes. Purpose: To investigate the influence of gene expression of chondrocytes at the time of transplantation on MRI outcomes up to 2 years after MACT. Study Design: Case series; Level of evidence, 4. Methods: This study included 25 patients with 27 symptomatic traumatic defects of articular cartilage, who had undergone MACT in the knee. Postoperative MRI examinations were conducted at 3, 6, 12, and 24 months after surgery. Biochemical graft maturation was assessed by measuring T2 relaxation time values of the transplant and healthy native cartilage areas. The MOCART (magnetic resonance observation of cartilage repair tissue) score was used to evaluate the morphological quality of regeneration tissue. Gene expression (collagen type I, collagen type II, aggrecan, versican, and interleukin-1β) was determined by real-time polymerase chain reaction (PCR) in transplant residuals at the time point of transplantation and was correlated with MRI outcomes using Spearman’s rank correlation coefficient. A Friedman test with post hoc analysis (Wilcoxon signed rank test) conducted with a Bonferroni correction was applied to compare scores at different time points. Results: T2 relaxation time of regeneration tissue improved from a mean ± SD of 74.6 ± 20.1 milliseconds at 3 months to 47.9 ±13.3 milliseconds at 24 months ( P < .003). These values were similar to the T2 relaxation times of the native surrounding cartilage (50.9 ± 15 ms). The calculated T2 index (ratio of regeneration tissue to native cartilage) improved from 1.63 ± 0.76 at 3 months to 1.0 ± 0.4 at 24 months ( P < .011). The MOCART score increased from 51.6 ± 15 points to 72.4 ± 12.2 points ( P < .001). Improvement of the T2 index over time significantly correlated with aggrecan, COL1A1, COL2A1, and versican expression ( rs = 0.9, P < .001; rs = 0.674, P < .012; rs = 0.553, P < .05; and rs = 0.575, P < .04, respectively). No correlation was found for IL-1β. Conclusion: These data demonstrate that matrix production in transplanted chondrocytes affects maturation of MACT grafts in MRI 2 years after surgery.

Functional Outcome of Knee Articular Cartilage Repair in Adolescent Athletes

2005 ◽  
Vol 33 (8) ◽  
pp. 1147-1153 ◽  
Author(s):  
Kai Mithöfer ◽  
Tom Minas ◽  
Lars Peterson ◽  
Howard Yeon ◽  
Lyle J. Micheli
Keyword(s):  
Articular Cartilage ◽  
Functional Outcome ◽  
Cartilage Repair ◽  
Autologous Chondrocyte Transplantation ◽  
Full Thickness ◽  
Chondrocyte Transplantation ◽  
Adolescent Athletes ◽  
Cartilage Lesions ◽  
Articular Cartilage Lesions ◽  
Autologous Chondrocyte

Background Limited information exists about the treatment of full-thickness articular cartilage lesions of the knee in adolescent athletes. Purpose To evaluate the functional outcome and athletic activity after articular cartilage repair in the knees of adolescent athletes. Study Design Case series; Level of evidence, 4. Methods Twenty adolescent athletes with full-thickness articular cartilage lesions of the knee were treated with autologous chondrocyte transplantation. Functional outcome was evaluated by subjective patient outcome rating, knee activity scores, and level of athletic participation. Results At a mean of 47 months after autologous chondrocyte transplantation, 96% of adolescents reported good or excellent results with significant increases in postoperative Tegner activity scores and Lysholm scores. Ninety-six percent returned to high-impact sports and 60% to an athletic level equal or higher than that before knee injury. Return to preinjury sports correlated with shorter preoperative symptoms and a lower number of prior operations. All adolescents with preoperative symptoms ≤12 months returned to preinjury-level athletics, compared to 33% with preoperative intervals longer than 12 months. Conclusion Treatment of full-thickness articular injuries of the knee in adolescent athletes with autologous chondrocyte transplantation yields a high rate of functional success at a mean follow-up of 47 months. The rate of return to demanding athletic activities is higher in cases in which the preoperative duration of symptoms is 12 months or less.

scholarly journals Analysis of graft maturation after autologous chondrocyte implantation with T2 mapping

2017 ◽  
Vol 5 (4_suppl4) ◽  
pp. 2325967117S0015
Author(s):  
Thomas R. Niethammer ◽  
Ansgar Loitzsch ◽  
Matthias F. Pietschmann ◽  
Peter E. Müller ◽  
Annie Horng
Keyword(s):  
Relaxation Time ◽  
Autologous Chondrocyte Implantation ◽  
T2 Mapping ◽  
Cartilage Defects ◽  
T2 Relaxation Time ◽  
T2 Relaxation ◽  
Postoperative Course ◽  
Defect Localization ◽  
Chondrocyte Implantation ◽  
Autologous Chondrocyte

Aims and Objectives: It has been shown in numerous clinical studies that matrix-based autologous chondrocyte implantation (mb-ACI) leads to good clinical results in the medium-term course. But chondrogenic development of ACI grafts and the time of graft maturation are still unclear. The aim of this study was to investigate the graft maturation after mb-ACI with T2 mapping in the postoperative course of four years. Materials and Methods: In a total of 90 patients could be included after matrix-based autologous chondrocyte transplantation of the knee. All cartilage defects were treated with NOVOCART® 3D. The evaluation of the ACI grafts was conducted after 3, 6, 12, 24, 36 and 48 months by MRI. The T2 relaxation time values of the ACI graft and healthy native cartilage areas were determined to assess graft maturation using T2 mapping. MOCART score were used for morphological evaluation of ACI grafts. Results: The T2 relaxation time of ACI grafts showed a significant improvement during the postoperative course at all time points. The T2 relaxation improved from 48.0 ms after 3 months to 34.6 ms after four years. We found a significant influence of the patient’s age and the defect localization on the T2 relaxation time measuring. It could be detected an increasing integration of the ACI grafts into the healthy surrounding cartilage. No significant differences between the T2 relaxation time of ACI grafts and the surrounding healthy cartilage were found after 12 months postoperatively. Conclusion: Graft maturation after ACI in the knee joint needs at least 1 year, with ongoing adjustment of the T2 relaxation time values compared with native surrounding cartilage. Regarding the age and defect localization, significantly worse T2 relaxation times of the ACI grafts could be found with higher age and in cases with treated patellar defects.

Articular Cartilage Repair in Soccer Players with Autologous Chondrocyte Transplantation

2005 ◽  
Vol 33 (11) ◽  
pp. 1639-1646 ◽  
Author(s):  
Kai Mithöfer ◽  
Lars Peterson ◽  
Bert R. Mandelbaum ◽  
Tom Minas
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Rating Scale ◽  
Skill Level ◽  
Soccer Players ◽  
Autologous Chondrocyte Transplantation ◽  
Chondrocyte Transplantation ◽  
Activity Rating ◽  
Articular Cartilage Lesions ◽  
Autologous Chondrocyte

Background The ability of autologous chondrocyte transplantation to produce and maintain an effective articular cartilage repair under high mechanical demands has not been investigated. Hypothesis Autologous chondrocyte transplantation provides a reliable and durable repair of full-thickness knee articular cartilage lesions in high-demand athletes. Study Design Case series; Level of evidence, 4. Methods A total of 45 soccer players were evaluated 41 ± 4 months after autologous chondrocyte transplantation for their ability to return to soccer, the timing of their return, skill level, and functional outcome rating by the Tegner activity rating scale score and Brittberg score. The factors influencing the return to sport were analyzed. Results Of these players, 72% reported good to excellent results, with significant overall improvement of Tegner activity rating scale scores; 33% returned to soccer, including 83% of competitive-level players and 16% of recreational players. Of the returning players, 80% returned to the same skill level and 87% maintained their ability to play soccer at 52 ± 8 months postoperatively. Players who successfully returned to soccer were significantly younger and had a shorter preoperative duration of symptoms than did patients who did not return to the sport. Concomitant adjuvant procedures did not adversely affect the ability to return to soccer. Conclusion Repair of knee articular cartilage lesions by autologous chondrocyte transplantation in high-performance athletes is particularly successful in younger, competitive athletes with limited preoperative intervals.

Sign in / Sign up

Export Citation Format

Share Document