Photo-reduction of flavin mononucleotide to semiquinone form in LOV domain mutants of blue-light receptor phot from Chlamydomonas reinhardtii

2007 ◽  
Vol 87 (1) ◽  
pp. 37-48 ◽  
Author(s):  
S.-H. Song ◽  
B. Dick ◽  
A. Penzkofer ◽  
P. Hegemann
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Blue Light ◽  
Flavin Mononucleotide ◽  
Lov Domain ◽  
Blue Light Receptor ◽  
Light Receptor

Engineered Arabidopsis Blue Light Receptor LOV Domain Variants with Improved Quantum Yield, Brightness, and Thermostability

2019 ◽  
Vol 67 (43) ◽  
pp. 12037-12043 ◽  
Author(s):  
Sanghwan Ko ◽  
Bora Hwang ◽  
Jung-Hyun Na ◽  
Jisun Lee ◽  
Sang Taek Jung
Keyword(s):  
Quantum Yield ◽  
Blue Light ◽  
Lov Domain ◽  
Blue Light Receptor ◽  
Light Receptor

scholarly journals Phot-LOV1: Photocycle of a Blue-Light Receptor Domain from the Green Alga Chlamydomonas reinhardtii

2003 ◽  
Vol 84 (2) ◽  
pp. 1192-1201 ◽  
Author(s):  
Tilman Kottke ◽  
Joachim Heberle ◽  
Dominic Hehn ◽  
Bernhard Dick ◽  
Peter Hegemann
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Blue Light ◽  
Green Alga ◽  
Blue Light Receptor ◽  
Light Receptor ◽  
Receptor Domain

Light-dependent suppression of COP1 multimeric complex formation is determined by the blue-light receptor FKF1 in Arabidopsis

2019 ◽  
Vol 508 (1) ◽  
pp. 191-197 ◽  
Author(s):  
Byoung-Doo Lee ◽  
Joon-Yung Cha ◽  
Mi Ri Kim ◽  
Gyeong-Im Shin ◽  
Nam-Chon Paek ◽  
...  
Keyword(s):  
Complex Formation ◽  
Blue Light ◽  
Blue Light Receptor ◽  
Multimeric Complex ◽  
Light Receptor

scholarly journals Tailored flavoproteins acting as light-driven spin machines pump nuclear hyperpolarization

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yonghong Ding ◽  
Alexey S. Kiryutin ◽  
Ziyue Zhao ◽  
Qian-Zhao Xu ◽  
Kai-Hong Zhao ◽  
...  
Keyword(s):  
Slow Motion ◽  
High Gain ◽  
Magnetic Interactions ◽  
Flavin Mononucleotide ◽  
Photosynthetic Reaction Centers ◽  
Lov Domain ◽  
Blue Light Receptor ◽  
Chemically Induced ◽  
High Resolution Nmr ◽  
Molecular Spin

Abstract The solid-state photo-chemically induced dynamic nuclear polarization (photo-CIDNP) effect generates non-Boltzmann nuclear spin magnetization, referred to as hyperpolarization, allowing for high gain of sensitivity in nuclear magnetic resonance (NMR). Well known to occur in photosynthetic reaction centers, the effect was also observed in a light-oxygen-voltage (LOV) domain of the blue-light receptor phototropin, in which the functional cysteine was removed to prevent photo-chemical reactions with the cofactor, a flavin mononucleotide (FMN). Upon illumination, the FMN abstracts an electron from a tryptophan to form a transient spin-correlated radical pair (SCRP) generating the photo-CIDNP effect. Here, we report on designed molecular spin-machines producing nuclear hyperpolarization upon illumination: a LOV domain of aureochrome1a from Phaeodactylum tricornutum, and a LOV domain named 4511 from Methylobacterium radiotolerans (Mr4511) which lacks an otherwise conserved tryptophan in its wild-type form. Insertion of the tryptophan at canonical and novel positions in Mr4511 yields photo-CIDNP effects observed by 15N and 1H liquid-state high-resolution NMR with a characteristic magnetic-field dependence indicating an involvement of anisotropic magnetic interactions and a slow-motion regime in the transient paramagnetic state. The heuristic biomimetic design opens new categories of experiments to analyze and apply the photo-CIDNP effect.

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