Molecular characterization of PVAS3: An asparagine synthetase gene from common bean prevailing in developing organs

Abstract The implementation of molecular tools that help the early selection of genotypes carrying target alleles increases efficiency and reduces the time and costs of breeding programs. The present study aimed the molecular characterization and validation of SNPs targeting disease resistance alleles for assisted selection. A total of 376 common bean lines with contrasting responses for anthracnose and angular leaf spot resistance were used, as well as 149 F2 plants from the cross between BRS Cometa x SEL 1308 (carrying the Anthracnose resistance gene Co-42). Seven of the ten SNP markers evaluated showed potential for assisted breeding: snpPV0025 (Phg-2), snpPV0027 (Phg-5), snpPV0079 (Phg-5), snpPV0046 (Co-u), snpPV0068 (Co-42), snpPV0070 (Co-42) and snpP8282v3-817 (Co-42). Markers snpPV0070 and snpP8282v3-817 showed high efficiency of selection (99.7 and 99.8%, respectively). These markers exhibit great potential to assist in the selection at different stages of the breeding program and may be readily incorporated into marker-assisted selection.

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Phenotypic and molecular characterization of cultivar BRSMGTalismã regarding the principal common bean pathogens

Cropp Breeding and Applied Biotechnology ◽

10.12702/1984-7033.v05n02a17 ◽

2005 ◽

Vol 5 (2) ◽

pp. 247-252 ◽

Cited By ~ 4

Author(s):

T.L.P.O. Souza ◽

V.A. Ragagnin ◽

L.P. Melo ◽

K.M.A. Arruda ◽

J.E.S. Carneiro ◽

...

Keyword(s):

Common Bean ◽

Molecular Characterization

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Morphological and Molecular Characterization of Common Bean Landraces Varieties in South of Brazil

10.21203/rs.3.rs-671577/v1 ◽

2021 ◽

Author(s):

Ana Claudia Schllemer dos Santos ◽

Lucas Vinícius de Sousa Alcântara ◽

Larissa Yuki Terada ◽

Isadora Bischoff Nunes ◽

Lucas da Silva Domingues ◽

...

Keyword(s):

Common Bean ◽

Molecular Characterization ◽

Morphological Characters ◽

Genetic Distances ◽

Maturation Stage ◽

Maturation Stages ◽

And Cluster Analysis ◽

South Of Brazil ◽

Morphological And Molecular Characterization

Abstract This work aimed to perform the morphological and molecular characterization of 31 land races varieties and 9 cultivars of common bean (Phaseolus vulgaris L.). Morphological characters were evaluated during seedling, flowering, physiological maturation and post-harvest maturation stages. Ten microsatellite markers were used for molecular screening. The markers were analyzed according to the number of alleles per locus, the allele frequency per locus and the polymorphism in content (PIC). Genetic distances and cluster analysis were performed using the Bayesian inference and the UPGMA method. All black beans evaluated have anthocyanin in the cotyledons, hypocotyls and stem, and their flowers are purple. For the growth habit, 50% of the evaluated genotypes belong to the type III and there is a predominance of yellow color in the pods (85%) at the physiological maturation stage. Through the genetic dissimilarity it was possible to discriminate three groups for the Likelihood reason and six groups through the UPGMA method, these results indicate a wide genetic diversity among the evaluated genotypes.

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