Bovine respiratory syncytial virus (BRSV) is a pneumovirus in the family
paramyxoviridae, is an important cause of acute respiratory disease in
postweaning calves and feedlot cattle. The real-time reverse transcriptase
PCR protocols were developed to detect BRSV infection in infected animals.
The sensitivity of RT-PCR protocols based on fusion gene were evaluated using
different Mastermixes such as Qiagen One Step RT-PCR (Qiagen) for
conventional RT-PCR, Superscrip probe (Invitrogen) and QuentiTec probe
(Qiagen) for realtime RT-PCR with and without internal control. The detection
limit of different RT-PCR protocols using serial dilutions from BRSV plasmid
and based on different probes was 10 RNA copies/ml. Furthermore, the
specificity of real-time RT-PCR was evaluated using different bacterial and
viral strains which can be isolated from respiratory infected animals. In
another side, the real-time RT-PCR in combination with ?-actin and
conventional RT-PCR showed detectable Ctvalues only with BRSV strain.
Detection and quantitation of bovine respiratory syncytial virus using real-time quantitative RT-PCR and quantitative competitive RT-PCR assays
