Strand-specific, real-time RT-PCR assays for quantification of genomic and positive-sense RNAs of the fish rhabdovirus, Infectious hematopoietic necrosis virus

2006 ◽  
Vol 132 (1-2) ◽  
pp. 18-24 ◽  
Author(s):  
Maureen K. Purcell ◽  
S. Alexandra Hart ◽  
Gael Kurath ◽  
James R. Winton
Keyword(s):  
Real Time ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Rt Pcr ◽  
Necrosis Virus ◽  
Positive Sense ◽  
Infectious Hematopoietic Necrosis ◽  
Pcr Assays

scholarly journals Universal reverse-transcriptase real-time PCR for infectious hematopoietic necrosis virus (IHNV)

2013 ◽  
Vol 106 (2) ◽  
pp. 103-115 ◽  
Author(s):  
MK Purcell ◽  
RL Thompson ◽  
KA Garver ◽  
LM Hawley ◽  
WN Batts ◽  
...  
Keyword(s):  
Reverse Transcriptase ◽  
Real Time ◽  
Real Time Pcr ◽  
Infectious Hematopoietic Necrosis Virus ◽  
Necrosis Virus ◽  
Infectious Hematopoietic Necrosis

scholarly journals Comparison of nine different commercially available molecular assays for detection of SARS-CoV-2 RNA

2021 ◽  
Author(s):  
Ute Eberle ◽  
◽  
Clara Wimmer ◽  
Ingrid Huber ◽  
Antonie Neubauer-Juric ◽  
...  
Keyword(s):  
Real Time ◽  
Rt Pcr ◽  
Diagnostic Assays ◽  
Molecular Assays ◽  
Pcr Assays ◽  
Laboratory Experiences

AbstractTo face the COVID-19 pandemic, the need for fast and reliable diagnostic assays for the detection of SARS-CoV-2 is immense. We describe our laboratory experiences evaluating nine commercially available real-time RT-PCR assays. We found that assays differed considerably in performance and validation before routine use is mandatory.

scholarly journals Quantitation of replication of the HCV genome in human livers with end-stage cirrhosis by strand-specific real-time RT-PCR assays: Methods and clinical relevance

2009 ◽  
Vol 81 (9) ◽  
pp. 1569-1575 ◽  
Author(s):  
Lan Lin ◽  
Louis Libbrecht ◽  
Jannick Verbeeck ◽  
Chris Verslype ◽  
Tania Roskams ◽  
...  
Keyword(s):  
Real Time ◽  
Clinical Relevance ◽  
Rt Pcr ◽  
Pcr Assays ◽  
Human Livers ◽  
End Stage

Validation and comparison of different end point and real time RT-PCR assays for detection and genotyping of porcine reproductive and respiratory syndrome virus

2014 ◽  
Vol 201 ◽  
pp. 79-85 ◽  
Author(s):  
Michele Drigo ◽  
Giovanni Franzo ◽  
Ilaria Belfanti ◽  
Marco Martini ◽  
Alessandra Mondin ◽  
...  
Keyword(s):  
Real Time ◽  
Respiratory Syndrome Virus ◽  
Rt Pcr ◽  
End Point ◽  
Pcr Assays
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