Enhanced immunogenicity of multiple-epitopes of foot-and-mouth disease virus fused with porcine interferon α in mice and protective efficacy in guinea pigs and swine

Objectives: Foot-and-mouth disease virus (FMDV) causes a highly contagious disease in cloven-hoofed animals and is the most damaging disease of livestock worldwide, leading to great economic losses. The aim of this research was the inactivation of FMDV type O/IRN/1/2007 to produce a gamma ray-irradiated (GRI) vaccine in order to immunize mice and guinea pigs. Methods: In this research, the Iranian isolated FMDV type O/IRN/1/2007 was irradiated by gamma ray to prepare an inactivated whole virus antigen and formulated as a GRI vaccine with unaltered antigenic characteristics. Immune responses against this vaccine were evaluated on mice and guinea pigs. Results: The comparison of the immune responses between the GRI vaccine and conventional vaccine did not show any significant difference in neutralizing antibody titer, memory spleen T lymphocytes or IFN-γ, IL-4, IL-2 and IL-10 concentrations (p > 0.05). In contrast, there were significant differences in all of the evaluated immune factors between the two vaccinated groups of mice and negative control mice (p < 0.05). The protective dose 50 for the conventional and GRI vaccines obtained were 6.28 and 7.07, respectively, which indicated the high potency of both vaccines. Conclusion: GRI vaccine is suitable for both routine vaccination and control of FMDV in emergency outbreaks.

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Ultrastructural changes and antigen localization in tissues from foot-and-mouth disease virus-infected guinea-pigs

Veterinary Microbiology ◽

10.1016/0378-1135(82)90055-4 ◽

1982 ◽

Vol 7 (5) ◽

pp. 391-400 ◽

Cited By ~ 3

Author(s):

S.H. Wool ◽

J. Polatnick ◽

R.C. Knudsen

Keyword(s):

Disease Virus ◽

Guinea Pigs ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Ultrastructural Changes ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Antigen Localization

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Chimeric virus-like particles elicit protective immunity against serotype O foot-and-mouth disease virus in guinea pigs

Applied Microbiology and Biotechnology ◽

10.1007/s00253-017-8246-0 ◽

2017 ◽

Vol 101 (12) ◽

pp. 4905-4914 ◽

Cited By ~ 8

Author(s):

Xinsheng Liu ◽

Yuzhen Fang ◽

Peng Zhou ◽

Yanzhen Lu ◽

Qiaoling Zhang ◽

...

Keyword(s):

Disease Virus ◽

Guinea Pigs ◽

Protective Immunity ◽

Foot And Mouth Disease ◽

Mouth Disease ◽

Chimeric Virus ◽

Virus Like Particles ◽

Serotype O ◽

Mouth Disease Virus ◽

Foot And Mouth

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Intranasal Immunization of Guinea Pigs with an Immunodominant Foot-and-Mouth Disease Virus Peptide Conjugate Induces Mucosal and Humoral Antibodies and Protection against Challenge

Journal of Virology ◽

10.1128/jvi.77.13.7486-7491.2003 ◽

2003 ◽

Vol 77 (13) ◽

pp. 7486-7491 ◽

Cited By ~ 21

Author(s):

D. Fischer ◽

D. Rood ◽

R. W. Barrette ◽

A. Zuwallack ◽

E. Kramer ◽

...

Keyword(s):

Disease Virus ◽

Guinea Pigs ◽

Neutralizing Antibodies ◽

Immunoglobulin A ◽

Foot And Mouth Disease ◽

Keyhole Limpet Hemocyanin ◽

Mouth Disease ◽

Mouth Disease Virus ◽

Foot And Mouth ◽

Nasal Secretions

ABSTRACT Guinea pigs immunized intranasally with a keyhole limpet hemocyanin-linked peptide, corresponding to the prominent G-H loop of the VP1 protein of foot-and-mouth disease virus, raised substantial levels of antipeptide and virus-neutralizing antibodies in sera and of peptide-specific secretory immunoglobulin A in nasal secretions. In groups of animals immunized intranasally without adjuvant, 86 percent were fully protected upon challenge with homotypic virus. Surprisingly, animals given the peptide conjugates plus the mucosal adjuvant cholera toxin were afforded only partial protection in that primary lesions were observed in most animals, although spread to other feet was prevented. These results indicate that intranasal inoculation with the peptide offers a potential route of vaccination against foot-and-mouth disease and may be useful for eliciting protection in the upper respiratory tracts of susceptible animals.

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