Construction and characterization of HIV type 1 CRF07_BC infectious molecular clone from men who have sex with men

2016 ◽  
Vol 229 ◽  
pp. 70-77 ◽  
Author(s):  
Yan-Ling Jiang ◽  
Wen-Wei Bai ◽  
Fan-Wei Qu ◽  
Hua Ma ◽  
Run-Sheng Jiang ◽  
...  
Keyword(s):  
Infectious Molecular Clone ◽  
Molecular Clone ◽  
Hiv Type 1 ◽  
Sex With Men

Construction and Characterization of an Infectious Molecular Clone of HIV Type 1 CRF07_BC

2008 ◽  
Vol 24 (2) ◽  
pp. 259-264 ◽  
Author(s):  
Zhefeng Meng ◽  
Xiang He ◽  
Hui Xing ◽  
Ruolei Xin ◽  
Jianping Sun ◽  
...  
Keyword(s):  
Infectious Molecular Clone ◽  
Molecular Clone ◽  
Hiv Type 1

Construction and Characterization of an Infectious Molecular Clone Derived from the CRF01_AE Primary Isolate of HIV Type 1

2002 ◽  
Vol 18 (8) ◽  
pp. 585-589 ◽  
Author(s):  
Tetsu Mukai ◽  
Satoshi Komoto ◽  
Takeshi Kurosu ◽  
J. Alejandro Palacios ◽  
Yong-Gang Li ◽  
...  
Keyword(s):  
Primary Isolate ◽  
Infectious Molecular Clone ◽  
Molecular Clone ◽  
Hiv Type 1

scholarly journals Near Full-Length Genomic Characterization of a Novel HIV Type 1 CRF07_ BC/01_AE Recombinant in Men Who Have Sex with Men from Sichuan, China

2013 ◽  
Vol 29 (8) ◽  
pp. 1173-1176 ◽  
Author(s):  
Huamian Wei ◽  
Ling Su ◽  
Yi Feng ◽  
Xiang He ◽  
Jenny His ◽  
...  
Keyword(s):  
Full Length ◽  
Genomic Characterization ◽  
Hiv Type 1 ◽  
Sex With Men

Short Communication: Construction and Characterization of a Stable Full-Length Macrophage-Tropic HIV Type 1 Molecular Clone That Directs the Production of High Titers of Progeny Virions

1996 ◽  
Vol 12 (3) ◽  
pp. 191-194 ◽  
Author(s):  
THEODORE S. THEODORE ◽  
GEORGE ENGLUND ◽  
ALICIA BUCKLER-WHITE ◽  
CHARLES E. BUCKLER ◽  
MALCOLM A. MARTIN ◽  
...  
Keyword(s):  
Short Communication ◽  
Full Length ◽  
Molecular Clone ◽  
Hiv Type 1

Isolation and Characterization of an Infectious HIV Type 1 Molecular Clone from a Patient with Primary Infection

2002 ◽  
Vol 18 (15) ◽  
pp. 1127-1133 ◽  
Author(s):  
Hiroaki Takeuchi ◽  
Youichi Suzuki ◽  
Masashi Tatsumi ◽  
Hiroo Hoshino ◽  
Eric S. Daar ◽  
...  
Keyword(s):  
Primary Infection ◽  
Molecular Clone ◽  
Isolation And Characterization ◽  
Hiv Type 1

Molecular Characterization of Variable Heavy and Light Chain Regions of Five HIV Type 1-Specific Human Monoclonal Antibodies*

1994 ◽  
Vol 10 (12) ◽  
pp. 1639-1649 ◽  
Author(s):  
ERIC M.M. van der DONK ◽  
MARTIN SCHUTTEN ◽  
ALBERT D.M.E. OSTERHAUS ◽  
ROGER W.J. van der HEIJDEN
Keyword(s):  
Monoclonal Antibodies ◽  
Molecular Characterization ◽  
Light Chain ◽  
Human Monoclonal Antibodies ◽  
Variable Heavy ◽  
Hiv Type 1

scholarly journals Construction of an Excisable Bacterial Artificial Chromosome Containing a Full-Length Infectious Clone of Herpes Simplex Virus Type 1: Viruses Reconstituted from the Clone Exhibit Wild-Type Properties In Vitro and In Vivo

2003 ◽  
Vol 77 (2) ◽  
pp. 1382-1391 ◽  
Author(s):  
Michiko Tanaka ◽  
Hiroyuki Kagawa ◽  
Yuji Yamanashi ◽  
Tetsutaro Sata ◽  
Yasushi Kawaguchi
Keyword(s):  
Vero Cells ◽  
Infectious Molecular Clone ◽  
Wild Type ◽  
Molecular Clone ◽  
Viral Genes ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT In recent years, several laboratories have reported on the cloning of herpes simplex virus type 1 (HSV-1) genomes as bacterial artificial chromosomes (BACs) in Escherichia coli and on procedures to manipulate these genomes by using the bacterial recombination machinery. However, the HSV-BACs reported so far are either replication incompetent or infectious, with a deletion of one or more viral genes due to the BAC vector insertion. For use as a multipurpose clone in research on HSV-1, we attempted to generate infectious HSV-BACs containing the full genome of HSV-1 without any loss of viral genes. Our results were as follows. (i) E. coli (YEbac102) harboring the full-length HSV-1 genome (pYEbac102) in which a BAC flanked by loxP sites was inserted into the intergenic region between UL3 and UL4 was constructed. (ii) pYEbac102 was an infectious molecular clone, given that its transfection into rabbit skin cells resulted in production of infectious virus (YK304). (iii) The BAC vector sequence was almost perfectly excisable from the genome of the reconstituted virus YK304 by coinfection of Vero cells with YK304 and a recombinant adenovirus, AxCANCre, expressing Cre recombinase. (iv) As far as was examined, the reconstituted viruses from pYEbac102 could not be phenotypically differentiated from wild-type viruses in vitro and in vivo. Thus, the viruses grew as well in Vero cells as did the wild-type virus and exhibited wild-type virulence in mice on intracerebral inoculation. (v) The infectious molecular clone pYEbac102 is in fact useful for mutagenesis of the HSV-1 genome by bacterial genetics, and a recombinant virus carrying amino acid substitutions in both copies of the α0 gene was generated. pYEbac102 will have multiple applications to the rapid generation of genetically engineered HSV-1 recombinants in basic research into HSV-1 and in the development of HSV vectors in human therapy.

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