Efficient virus-induced gene silencing system in pumpkin (Cucurbita maxima) using apple latent spherical virus vector

2022 ◽  
pp. 114456
Author(s):  
Noriko Yamagishi ◽  
Nobuyuki Yoshikawa
2016 ◽  
Vol 199 ◽  
pp. 103-113 ◽  
Author(s):  
Takashi Kawai ◽  
Ayako Gonoi ◽  
Michiya Nitta ◽  
Noriko Yamagishi ◽  
Nobuyuki Yoshikawa ◽  
...  

2019 ◽  
Vol 273 ◽  
pp. 113711 ◽  
Author(s):  
Chunjiang Li ◽  
Hiroto Hirano ◽  
Ichiro Kasajima ◽  
Noriko Yamagishi ◽  
Nobuyuki Yoshikawa

2016 ◽  
pp. pp.00172.2016 ◽  
Author(s):  
Yu Mei ◽  
Chunquan Zhang ◽  
Bliss M. Kernodle ◽  
John H. Hill ◽  
Steven A. Whitham

HortScience ◽  
2012 ◽  
Vol 47 (9) ◽  
pp. 1278-1282 ◽  
Author(s):  
Hiroaki Ito ◽  
Masaki Ochiai ◽  
Hiroaki Kato ◽  
Katsuhiro Shiratake ◽  
Daigo Takemoto ◽  
...  

We have succeeded in establishing a virus-induced gene silencing (VIGS) of rose using Apple latent spherical virus (ALSV) vectors. An ALSV infection on rose did not cause any symptoms like those observed on other plant species and grew healthy. We have cloned and sequenced the phytoene desaturase (PDS) gene in wild rose, then used its fragment for silencing the rose internal PDS gene. The silencing phenotypes such as the highly uniform photo-bleached phenotype with PDS inhibitions were observed on the upper leaves of primary shoots and on a secondary shoot of R. rugosa for more than 5 months. ALSV vectors seemed useful for analyzing gene function and for the molecular breeding of rose.


PLoS ONE ◽  
2011 ◽  
Vol 6 (10) ◽  
pp. e26468 ◽  
Author(s):  
Cheng Yuan ◽  
Cui Li ◽  
Lijie Yan ◽  
Andrew O. Jackson ◽  
Zhiyong Liu ◽  
...  

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