Based on parts of R. Costanzas results of ecosystem services value, this paper mainly discusses the calculative method for the value of natural ecosystem services. Appling some relative research and used substitution cost methods, prevented cost methods, shadow price methods, indirect market methods to estimate influences on constructive land ecosystem services in order to provided the basis for modification of atmosphere and climate and applying substitution cost methods to estimate the values of water regulation. By taking Taicang county in Jiangsu province as an application example, by identifying the functions of ecosystem services within this zone, and by calculating the value of ecosystem services through different years in the whole region , the annual tendency was then analysed. The results indicate that unmodified eco-service value represent theoretical levels of eco-service value, and can not show the changes of the ecosystem as a result of outside influences. But when the degree and size of influences on ecosystem by human activities on the constructional land are taken into account, and the eco-services values are considered as the results of original eco-service value plus outside influencing actions, the modified valuation methods for eco-service Values seem fairly suitable for the ecosystem itself and for the actual eco-service values shown by the ecosystem.