scholarly journals Characterization of Met25 as a color associated genetic marker in Yarrowia lipolytica

2020 ◽  
Vol 11 ◽  
pp. e00147
Author(s):  
Harley Edwards ◽  
Zhiliang Yang ◽  
Peng Xu
2001 ◽  
Vol 47 (9) ◽  
pp. 861-870 ◽  
Author(s):  
Pascale Jolivet ◽  
Edith Bergeron ◽  
Haguith Benyair ◽  
Jean-Claude Meunier

Casein phosphatase activities have been identified in five yeast strains grown on Pi-deficient medium. Maximal endocellular activities appeared in the exponential phase. Exocellular phosphatases were significantly produced from Yarrowia lipolytica W-29 and Kluyveromyces marxianus, in the early stationary phase. Major phosphatases from K. marxianus were one heavy acid phosphatase composed of 64–67 kDa subunits, which could be secreted in the medium, and one type 2A protein phosphatase with an apparent molecular mass of 147 kDa and a 52 kDa catalytic subunit dissociated by 80% ethanol treatment. The characteristics of phosphatases purified from K. marxianus were compared with those previously purified from Y. lipolytica.Key words: yeast, type 2A protein phosphatase, acid phosphatase, [32P]casein, Pi deficiency.


PLoS ONE ◽  
2012 ◽  
Vol 7 (11) ◽  
pp. e50413 ◽  
Author(s):  
Felipe L. Assis ◽  
Gabriel M. F. Almeida ◽  
Danilo B. Oliveira ◽  
Ana P. M. Franco-Luiz ◽  
Rafael K. Campos ◽  
...  

2012 ◽  
Vol 49 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Hiroshi Takai ◽  
Ryo Iwama ◽  
Satoshi Kobayashi ◽  
Hiroyuki Horiuchi ◽  
Ryouichi Fukuda ◽  
...  

2009 ◽  
Vol 25 ◽  
pp. S138 ◽  
Author(s):  
P. Amaral ◽  
M. Colão ◽  
M.A. Coelho ◽  
G. Fontes ◽  
M. Nele
Keyword(s):  

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Imen Ben Tahar ◽  
Małgorzata Kus‐Liśkiewicz ◽  
Yannick Lara ◽  
Emmanuelle Javaux ◽  
Patrick Fickers

2007 ◽  
Vol 2007 ◽  
pp. 156-156 ◽  
Author(s):  
Masoud Alipanah ◽  
Lobov Kalashnikova ◽  
Genadi Rodionov

Prolactin plays an important regulatory function in mammary gland development, milk secretion, and expression of milk protein genes. Hence the PRL gene is a potential genetic marker of production traits in dairy cattle. The gene was mapped on chromosome 23 by Hallerman et al. (1988). It consists of 5 exons and four introns (Camper et al. 1984) encoding the 199-amino-acid mature protein (Wallis 1974). On the basis of sequence analysis of four different cDNA clones, seven possible nucleotide substitutions were described by Sasavage et al. (1982). One of them, recognized by RsaI endonuclease, has become a popular genetic marker used for genetic characterization of cattle populations by means of PCR-RFLP (Mitra et al., 1995). Two allelic variants (B and b) have been distinguished at the DNA level, based on RsaI polymorphism in the third exon of the coding region. It has been suggested that prolactin alleles correlate with milk yield (Lewin et al., 1992).


2014 ◽  
Vol 52 (4) ◽  
pp. 284-291 ◽  
Author(s):  
Jeong-Nam Park ◽  
Chang Pyo Han ◽  
Dong-Jik Lee ◽  
Seon Ah Cheon ◽  
Hyun Ah Kang

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