Single-use, single-pass tangential flow filtration using low-cost hollow fiber modules

2020 ◽  
Vol 595 ◽  
pp. 117517 ◽  
Author(s):  
Christopher J. Yehl ◽  
Andrew L. Zydney
Keyword(s):  
Hollow Fiber ◽  
Low Cost ◽  
Tangential Flow Filtration ◽  
Single Pass ◽  
Tangential Flow ◽  
Single Use ◽  
Flow Filtration

Investigation of single-pass tangential flow filtration (SPTFF) as an inline concentration step for cell culture harvest

2017 ◽  
Vol 524 ◽  
pp. 20-32 ◽  
Author(s):  
Abhiram Arunkumar ◽  
Nripen Singh ◽  
Michael Peck ◽  
Michael C. Borys ◽  
Zheng Jian Li
Keyword(s):  
Cell Culture ◽  
Tangential Flow Filtration ◽  
Single Pass ◽  
Tangential Flow ◽  
Concentration Step ◽  
Flow Filtration

scholarly journals Evaluation of Single-use Tangential Flow Filtration Technology for Purification of Activated Polysaccharides Used in Conjugate Vaccine Manufacturing

2021 ◽  
Author(s):  
Seyed Pouria Motevalian ◽  
Jonathan Steen ◽  
Janine De Leon ◽  
Verl Sriskanda ◽  
Ivette Carino ◽  
...  
Keyword(s):  
Process Development ◽  
Average Molecular Weight ◽  
Weighted Average ◽  
Process Performance ◽  
Product Attributes ◽  
Tangential Flow Filtration ◽  
Tangential Flow ◽  
Single Use ◽  
Flow Filtration ◽  
Manufacturing Time

Over the past decade, single-use tangential flow filtration (TFF) technologies have emerged to reduce system preparation time, promote fast and flexible product change over, and ultimately shorten process development and manufacturing time/cost. In this study, the performance of a recently developed Pellicon® single-use TFF capsule was compared against traditional Pellicon® cassette by assessing TFF process performance (such as flux, residuals clearance, and yield) and post-purification product attributes (such as concentration and mass-weighted average molecular weight). Good scaling was shown by comparing process performance and product attributes across different scales and formats. Additionally, similar TFF process performance and post-purification product attributes were observed for the single-use capsule compared to the reusable TFF cassettes. The capsule requires a smaller flush than the cassette, and it is easier to use since it does not require a compression holder or pre-sanitization. The results provide insight into the application of the single-use TFF capsule and scalability of TFF processes for the purification of conjugate vaccines.

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