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Water ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 190
Author(s):  
Qian Wang ◽  
Xiaobin Tang ◽  
Heng Liang ◽  
Wenjun Cheng ◽  
Guibai Li ◽  
...  

Gravity-driven membrane (GDM) filtration technology has been extensively in the employed drinking water treatment, however, the effect filtration mode (i.e., dead-end mode vs. cross-flow mode) on its long-term performance has not been systematically investigated. In this study, pilot-scale GDM systems were operated using two submerged filtration mode (SGDM) and cross-flow mode (CGDM) at the gravity-driven pressures 120 mbar and 200 mbar, respectively. The results showed that flux stabilization was observed both in the SGDM and CGDM during long-term filtration, and importantly the stabilized flux level of CGDM was elevated by 3.5–67.5%, which indicated that the filtration mode would not influence the occurrence of flux stability, but significantly improve the stable flux level. Interestingly, the stable flux level was not significantly improved with the increase of driven pressure, and the optimized driven pressure was 120 mbar. In addition, the GDM process conferred effective removals of turbidity, UV254, CODMn, and DOC, with average removals of 99%, 43%, 41%, and 20%, respectively. With the assistance of cross flow to avert the overaccumulation of contaminants on the membrane surface, CGDM process exhibited even higher removal efficiency than SGDM process. Furthermore, it can be found that the CGDM system can effectively remove the fluorescent protein-like substances, and the intensities of tryptophans substance and soluble microbial products were reduced by 64.61% and 55.08%, respectively, higher than that of the SGDM. Therefore, it can be determined that the filtration mode played an important role in the flux stabilization of GDM system during long-term filtration, and the cross-flow filtration mode can simultaneously improve the stabilized flux level and removal performance.


2022 ◽  
Vol 5 (1) ◽  
Author(s):  
Vladimir Riazanski ◽  
Gerardo Mauleon ◽  
Kilean Lucas ◽  
Samuel Walker ◽  
Adriana M. Zimnicka ◽  
...  

AbstractExtracellular vesicles (EVs) are cell-derived membranous structures carrying transmembrane proteins and luminal cargo. Their complex cargo requires pH stability in EVs while traversing diverse body fluids. We used a filtration-based platform to capture and stabilize EVs based on their size and studied their pH regulation at the single EV level. Dead-end filtration facilitated EV capture in the pores of an ultrathin (100 nm thick) and nanoporous silicon nitride (NPN) membrane within a custom microfluidic device. Immobilized EVs were rapidly exposed to test solution changes driven across the backside of the membrane using tangential flow without exposing the EVs to fluid shear forces. The epithelial sodium-hydrogen exchanger, NHE1, is a ubiquitous plasma membrane protein tasked with the maintenance of cytoplasmic pH at neutrality. We show that NHE1 identified on the membrane of EVs is functional in the maintenance of pH neutrality within single vesicles. This is the first mechanistic description of EV function on the single vesicle level.


Author(s):  
Madeline Fuchs ◽  
Rajan Bhawnani ◽  
Sobhana Alekhya Sripada ◽  
Jessica Molek ◽  
Mehdi Ghodbane

Opportunities for process intensification and increased productivity have made the field of Continuous Biomanufacturing an area of high interest and active research. Within the purification train of producing biologics, Tangential Flow Filtration (TFF) is typically employed after chromatographic separations, to increase drug substance concentration, making the process more economical and further meeting dosage specifications. In a batch operation, concentration occurs via recirculation of the feed material where desired output concentration is attained through multiple pump-passes over the TFF membrane, while steadily excluding the buffer. Single-Pass Tangential Flow Filtration (SPTFF) enables continuity of this process by achieving similar concentration factors through a single – pass over these membranes while operating at low feed flow rates. Our work elucidates the development of a mechanistic process model to predict SPTFF performance across a relatively wide design space using a first principles approach. The developed model is found to be accurate for a range of high feed flow rates but is inaccurate at flow rates below 25 L/m2/hr. At very low flow rates, small differences in the mass transfer coefficient have been observed to significantly alter the prediction of the retentate concentration. We thus describe the challenges in predictive process modeling of SPTFF in antibody biomanufacturing.


Nanomaterials ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 31
Author(s):  
Varvara Platania ◽  
Alexandra Kaldeli-Kerou ◽  
Theodora Karamanidou ◽  
Maria Kouki ◽  
Alexander Tsouknidas ◽  
...  

A lot of effort has been dedicated recently to provide a better insight into the mechanism of the antibacterial activity of silver nanoparticles (AgNPs) colloidal suspensions and their released silver ionic counterparts. However, there is no consistency regarding whether the antibacterial effect displayed at cellular level originates from the AgNPs or their ionic constitutes. To address this issue, three colloidal suspensions exhibiting different ratios of AgNPs/silver ions were synthesized by a wet chemistry method in conjunction with tangential flow filtration, and were characterized and evaluated for their antimicrobial properties against two gram-negative, Escherichia coli (E. coli) and Pseudomonas aeruginosa (P. aeruginosa), and two gram-positive, Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis), bacterial strains. The produced samples contained 25% AgNPs and 75% Ag ions (AgNP_25), 50% AgNPs and 50% Ag ions (AgNP_50), and 100% AgNPs (AgNP_100). The sample AgNP_100 demonstrated the lowest minimum inhibitory concentration values ranging from 4.6 to 15.6 ppm for all four bacterial strains, while all three samples indicated minimum bactericidal concentration (MBC) values ranging from 16.6 ppm to 62.5 ppm against all strains. An increase in silver ions content results in higher bactericidal activity. All three samples were found to lead to a significant morphological damage by disruption of the bacterial cell membranes as analyzed by means of scanning electron microscopy (SEM). The growth kinetics demonstrated that all three samples were able to reduce the bacterial population at a concentration of 3.1 ppm. SEM and growth kinetic data underline that S. epidermidis is the most sensitive among all strains against the investigated samples. Our results showed that all three AgNPs colloidal suspensions exhibited strong antibacterial properties and, thus, they can be applied in medical devices and antimicrobial control systems.


Membranes ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 3
Author(s):  
James C. Foster ◽  
Timothy A. DeVol ◽  
Scott M. Husson

This contribution describes the fabrication of plutonium-adsorptive membranes by non-solvent induced phase separation. The dope solution comprised poly(vinylidene fluoride) (PVDF) and a Pu-extractive copolymer additive of PVDF-g-poly(ethylene glycol methacrylate phosphate) (EGMP) in dimethylformamide (DMF). The effects of casting conditions on membrane permeability were determined for PVDF membranes prepared with 10 wt% PVDF-g-EGMP. Direct-flow filtration and alpha spectrometry showed that membranes containing the graft copolymer could recover Pu up to 59.9 ± 3.0% from deionized water and 19.3 ± 3.5% from synthetic seawater after filtering 10 mL of 0.5 Bq/mL 238Pu. SEM-EDS analysis indicated that the graft copolymer was distributed evenly throughout the entire depth of the copolymer membranes, likely attributing to the tailing observed in the alpha spectra for 238Pu. Despite the reduction in resolution, the membranes exhibited high Pu uptake at the conditions tested, and new membrane designs that promote copolymer surface migration are expected to improve alpha spectrometry peak energy resolutions. Findings from this study also can be used to guide the development of extractive membranes for chromatographic separation of actinides from contaminated groundwater sources.


Membranes ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 775
Author(s):  
Jieun Lee ◽  
Jiwon Na ◽  
Youngbin Baek

Sugar excipients such as sucrose and maltose are widely used for biopharmaceutical formulation to improve protein stability and to ensure isotonicity for administration. However, according to recent literature, pharmaceutical-grade sucrose contained nanoparticulate impurities (NPIs) that result in protein aggregation and degradation. The objective of this study was to evaluate the filtrate flux behavior of sugar solution during ultrafiltration (UF) and diafiltration (DF). Filtrate flux data were obtained using either a tangential flow filtration (TFF) system for DF experiments or a normal flow filtration system for UF experiments. In diafiltration experiments, which were performed using 7 g/L of human immunoglobulin G in a 20 mM histidine buffer with the 100 mM sucrose or maltose, the filtrate flux with sucrose solution decreased significantly. In contrast, the one with maltose solution was in good correspondence with the calculated filtrate flux accounting for the effects of solution viscosity. This large decline in the flux was also observed during UF experiments, in which the presence of NPIs was identified by dynamic light scattering analysis and by capturing an SEM image of the membrane surface after filtration. In addition, highly purified sucrose resulted in a much lower flux decline in TFF in the absence of NPIs. These results provide important insights into the factors governing the optimization of the UF/DF process using appropriate excipients for biopharmaceutical formulation.


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