scholarly journals Genetic stability and phytochemical analysis of the in vitro regenerated plants of Dendrobium nobile Lindl., an endangered medicinal orchid

Meta Gene ◽  
2014 ◽  
Vol 2 ◽  
pp. 489-504 ◽  
Author(s):  
Paromik Bhattacharyya ◽  
Suman Kumaria ◽  
Reemavareen Diengdoh ◽  
Pramod Tandon
2018 ◽  
Vol 135 (1) ◽  
pp. 111-118 ◽  
Author(s):  
Janhvi Mishra Rawat ◽  
Aakriti Bhandari ◽  
Susmita Mishra ◽  
Balwant Rawat ◽  
Ashok Kumar Dhakad ◽  
...  

2012 ◽  
Vol 14 (4) ◽  
pp. 617-623 ◽  
Author(s):  
J.S. Soares ◽  
Y.B.C.J. Rosa ◽  
R.M. Suzuki ◽  
S.P.Q. Scalon ◽  
E.J. Rosa Junior

O presente trabalho teve como objetivo estudar a influência dos reguladores vegetais BAP e GA3 como tratamentos pré-germinativos no processo de germinação e desenvolvimento inicial de plântulas de Dendrobium nobile, espécie importante pelas propriedades farmacológicas como anti-oxidante, vasodilatadora e até mesmo anti-cancerígena, além do valor ornamental. Os tratamentos pré-germinativos consistiram de BAP e GA3, separadamente, nas concentrações de 0,0; 1,0; 2,0 e 5,0 mg L-1. Após seis meses da semeadura in vitro e manutenção em câmara de germinação e de crescimento com temperatura e foto-período controlados (12 horas e 23ºC ± 2), foram avaliados os parâmetros número de sementes germinadas, porcentagem de germinação, massa fresca e altura das plântulas, diâmetro e número de pseudobulbos, número de folhas, número de raízes, e o comprimento da maior raiz. O delineamento experimental foi inteiramente casualizado. Todas as variáveis foram submetidas à análise de variância e de regressão, quando significativas. As sementes de D. nobile germinaram melhor na ausência de reguladores vegetais e os tratamentos com BAP ou GA3 na embebição das sementes pouco beneficiaram o desenvolvimento in vitro de D. nobile.


Meta Gene ◽  
2018 ◽  
Vol 18 ◽  
pp. 100-106 ◽  
Author(s):  
Samar Amin ◽  
Tareq A. Wani ◽  
Zahoor Ahmad Kaloo ◽  
Seema Singh ◽  
Riffat John ◽  
...  

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 1077C-1077
Author(s):  
Wenhao Dai ◽  
Victoria Magnusson ◽  
Andrea Swanberg

Many woody plants, including some birch species, can be cloned using such in vitro techniques as pre-existing meristem culture, organogenesis, and embryogenesis. However, clonal fidelity of in vitro-derived plants is always a big concern because somaclonal variations may be induced during the entire in vitro process. To address this issue, we used random amplified polymorphic DNA (RAPD) markers to determine the genetic stability of in vitro-propagated plants of Betula platyphylla `Fargo'. Forty-two greenhouse-grown birch plants derived from a 10-year shoot tip culture (shoot-derived) and 42 in vitro plants regenerated from leaf tissues (regenerated) were randomly selected and evaluated for their genetic fidelity by RAPD. To date, 20 primers (C1-C20, Operon Technologies) were screened for all 84 plants. Only strong bands that are conservative were scored. Each primer generated a unique set of amplification products. Most of scoreable bands are ranged from 350 to 1800 bp. A total of 3696 fragments were amplified from 42 shoot-derived plants by all 20 primers with an average of 4.4 bands per primer, in which 6 primers produced polymorphic bands, indicating some genetic variations within shoot-derived plants. Nineteen out of 20 primers yielded 2772 clear and reproducible bands (an average of 3.47 per primer) from 42 regenerated plants with no significant variations being detected. Our preliminary results showed that in vitro regenerated plants are genetically uniform. However, a long-term tissue culture might result in a few genetic variations of birch species.


3 Biotech ◽  
2012 ◽  
Vol 3 (3) ◽  
pp. 235-239 ◽  
Author(s):  
Padmaja Mohanty ◽  
Pynbeitsyon Nongkling ◽  
Meera C. Das ◽  
Suman Kumaria ◽  
Pramod Tandon

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