A fluorescent assay for the ultrasensitive detection of miRNA-21 is based on immobilization of PPIX as signal indicators in massive G-quadruplex structures obtained by target recycling, three-dimensional DNA walker and RCA coupled cascade nucleic acid amplification.
A self-powered microRNAs biosensor with triple signal amplification systems is assembled through integration of three-dimensional DNA walker, enzymatic biofuel cells and capacitor. The DNA walker is designed from an enzyme-free...