A novel fluorescent assay for the ultrasensitive detection of miRNA-21 with the use of G-quadruplex structures as an immobilization material for a signal indicator

2019 ◽  
Vol 55 (45) ◽  
pp. 6453-6456 ◽  
Author(s):  
Ze-Zhou Yang ◽  
Zhi-Bin Wen ◽  
Xin Peng ◽  
Ya-Qin Chai ◽  
Wen-Bin Liang ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Three Dimensional ◽  
Nucleic Acid Amplification ◽  
Ultrasensitive Detection ◽  
Fluorescent Assay ◽  
Target Recycling ◽  
Dna Walker ◽  
G Quadruplex

A fluorescent assay for the ultrasensitive detection of miRNA-21 is based on immobilization of PPIX as signal indicators in massive G-quadruplex structures obtained by target recycling, three-dimensional DNA walker and RCA coupled cascade nucleic acid amplification.

scholarly journals Ultrasensitive Detection of Clostridium difficile Toxins Reveals Suboptimal Accuracy of Toxin Gene Cycle Thresholds for Toxin Predictions

2019 ◽  
Vol 57 (6) ◽  
Author(s):  
Johanna Sandlund ◽  
Mark H. Wilcox
Keyword(s):  
Clinical Practice ◽  
Nucleic Acid ◽  
Clostridium Difficile ◽  
Predictive Accuracy ◽  
High Sensitivity ◽  
Nucleic Acid Amplification ◽  
Toxin Gene ◽  
Ultrasensitive Detection ◽  
Surrogate Markers ◽  
Recent Interest

ABSTRACT The use of nucleic acid amplification tests (NAATs) for the diagnosis of Clostridium (Clostridioides) difficile infection (CDI) leads to overdiagnosis. To improve the clinical specificity of NAATs, there has been a recent interest in using toxin gene cycle thresholds (CTs) to predict the presence and absence of toxins. Although there is an association between CT values and fecal toxin concentrations, the predictive accuracy of the former is suboptimal for use in clinical practice. Ultrasensitive toxin immunoassays to quantify free toxins in stool offer a novel option for high-sensitivity fecal toxin detection rather than using surrogate markers for prediction.

An autocatalytic DNA machine with autonomous target recycling and cascade circular exponential amplification for one-pot, isothermal and ultrasensitive nucleic acid detection

2016 ◽  
Vol 52 (74) ◽  
pp. 11108-11111 ◽  
Author(s):  
Xinya Sun ◽  
Li Wang ◽  
Mingsha Zhao ◽  
Changzhi Zhao ◽  
Shufeng Liu
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acid Detection ◽  
Ultrasensitive Detection ◽  
One Pot ◽  
Target Recycling ◽  
Target Dna ◽  
Dna Machine ◽  
The One ◽  
Amplification Strategy

An autonomous target recycling and cascade circular exponential amplification strategy was proposed for the one-pot, isothermal and ultrasensitive detection of target DNA.

Flap endonuclease-initiated enzymatic repairing amplification for ultrasensitive detection of target nucleic acids

Nanoscale ◽  
2019 ◽  
Vol 11 (8) ◽  
pp. 3633-3638 ◽  
Author(s):  
Hyowon Jang ◽  
Chang Yeol Lee ◽  
Seoyoung Lee ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Nucleic Acid Amplification ◽  
Ultrasensitive Detection ◽  
Isothermal Nucleic Acid Amplification ◽  
Amplification Method

A new isothermal nucleic acid amplification method termed FERA (Flap endonuclease-initiated Enzymatic Repairing Amplification) is developed for the ultrasensitive detection of target nucleic acids.

scholarly journals Three-dimensional DNA nanostructures to improve the hyperbranched hybridization chain reaction

2019 ◽  
Vol 10 (42) ◽  
pp. 9758-9767 ◽  
Author(s):  
Jing Wang ◽  
Dong-Xia Wang ◽  
Jia-Yi Ma ◽  
Ya-Xin Wang ◽  
De-Ming Kong
Keyword(s):  
Nucleic Acid ◽  
Three Dimensional ◽  
Nucleic Acid Amplification ◽  
Hybridization Chain Reaction ◽  
Dna Nanostructures ◽  
Chain Reaction ◽  
Nucleic Acid Amplification Techniques ◽  
Detection Of Biomarkers

Nonenzymatic nucleic acid amplification techniques (e.g. the hybridization chain reaction, HCR) have shown promising potential for amplified detection of biomarkers.

A three-dimensional multipedal DNA walker for the ultrasensitive detection of tumor exosomes

2020 ◽  
Vol 56 (85) ◽  
pp. 12949-12952
Author(s):  
Huizhen Wang ◽  
Kejing Wan ◽  
Yue Zhou ◽  
Xiaoxiao He ◽  
Dinggeng He ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Ultrasensitive Detection ◽  
Dna Walker ◽  
Tumor Exosomes

Herein, we developed a three-dimensional (3D) multipedal DNA walker based on aptamer-binding for ultrasensitive exosome sensing.

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