Optimization of immobilization conditions of Mucor miehei lipase onto Florisil via polysuccinimide spacer arm using response surface methodology and application of immobilized lipase in asymmetric acylation of 2-amino-1-phenylethanols

2014 ◽  
Vol 100 ◽  
pp. 91-103 ◽  
Author(s):  
Deniz Yildirim ◽  
S. Seyhan Tükel ◽  
Özlem Alptekin ◽  
Dilek Alagöz
2004 ◽  
Vol 20 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Laura Maria Bruno ◽  
Gustavo Adolfo Saavedra Pinto ◽  
Heizir Ferreira de Castro ◽  
José Luiz de Lima-Filho ◽  
Eduardo Henrique de Magalhães Melo

2012 ◽  
Vol 6 (1) ◽  
pp. 77-85
Author(s):  
Imen Aissa ◽  
Mohamed Sellami ◽  
Amel Kamoun ◽  
Youssef Gargouri ◽  
Nabil Miled

2020 ◽  
Vol 163 ◽  
pp. 1719-1728
Author(s):  
Xiao-Tian Liu ◽  
Tian-Tian Liu ◽  
Hui-Long Xu ◽  
Qing-Xi Chen ◽  
Qin Wang

Energies ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 831 ◽  
Author(s):  
Juan Calero ◽  
Diego Luna ◽  
Carlos Luna ◽  
Felipa Bautista ◽  
Beatriz Hurtado ◽  
...  

Two inorganic solids have been evaluated as supports of Lipozyme RM IM, a Rhizomucor miehei lipase immobilized on a macroporous anion exchange resin, in order to improve its application as a biocatalyst in the synthesis of biofuels. The experimental conditions have been optimized to get the selective transesterification of sunflower oil, by using a multi-factorial design based on the response surface methodology (RSM). In this way, the effects of several reaction parameters on the selective ethanolysis of triglycerides to produce Ecodiesel, a biodiesel-like biofuel constitute by one mole of monoglyceride (MG) and two moles of fatty acid ethyl ester (FAEE), have been evaluated. Thus, it was obtained that a 6:1 oil/ethanol molar ratio, 0.215 g of biocatalyst supported in silica-gel (0.015 g Lipase/0.2 g silica-gel), 50 µL of 10 N NaOH, together with previous optimized reaction parameters, 35 °C reaction temperature and 120 min of reaction time, gave the best results (conversions around 70%; selectivity around 65%; kinematic viscosities about 9.3 mm2/s) in the reaction studied. Besides, Lipozyme RM IM, supported on silica-gel, biocatalyst exhibited a very good stability, remaining its activity even after 15 cycles.


Membranes ◽  
2012 ◽  
Vol 2 (2) ◽  
pp. 198-213 ◽  
Author(s):  
Nurrahmi Handayani ◽  
Katja Loos ◽  
Deana Wahyuningrum ◽  
Buchari ◽  
Muhammad Ali Zulfikar

2012 ◽  
Vol 6 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Imen Aissa ◽  
Mohamed Sellami ◽  
Amel Kamoun ◽  
Youssef Gargouri ◽  
Nabil Miled

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