Development of Local Functional Food Made of Buffalo Milk Improved with Culture Probiotics (Lactobacillus casei)

The research was conducted to develop functional food products of milk-based livestock origin (Semi hard-type cheese), with the addition of pure culture Lactobacillus casei as a probiotic agent, and citric acid and Mucor meihei as milk coagulants. The research material was semi-hard type cheese made of approximately 35 liters of buffalo milk from West Sumbawa Regency as a basic ingredient with the probiotic pure culture. The results showed that the pure culture of probiotic (Lactobacillus casei) at levels of 10% and 15% can survive and develop quite well in semi-hard cheese during aging, from 1 day, 7 days, and 14 days, respectively (3.79 – 5.92) and (4.91 – 6.31) log cfu g-1. While the 0.025% rennet of the volume of milk from Mucor miehei gives a pretty good result, it can be seen from the product recovery which can reach (34.30 + 0.32) %. During aging for 14 days, an organoleptic quality which includes aroma, color, and texture was getting better, with the criteria of a semi-hard aroma, yellowish-white color, and semi-hard texture. It can be concluded, that semi-hard type cheese can be used as one of the functional foods of probiotic carriers. To get the therapeutic effect, this probiotic should be consumed at least 100 grams per serving.

A Performance Study of Home-Made Co-Immobilized Lipase from Mucor miehei in Polyurethane Foam on The Hydrolysis of Coconut Oil to Fatty Acid

Bio‐based fatty acids (FAs) produced through hydrolysis of natural oils and fats are promising chemical feedstocks for increasing the economic value of renewable raw materials. In this work, lecithin, gelatin, PEG, and MgCl2 were employed as the co-immobilized material of crude lipase Mucor miehei immobilization on the polyurethane foam (PUF) matrix for hydrolysis of coconut oil to Free Fatty Acid (FFA). The unconventional immobilized technique was used through cross-linking and covalent bond. Single factor analysis and response surface method were utilized to determine the optimum conditions of the hydrolysis reaction. After optimization, co-immobilized lipase was examined for storage stability at a temperature of 4°C and reusability performance. The optimum conditions for coconut oil hydrolysis were obtained on the co-immobilized-PUF ratio, water-oil ratio, and reaction time of 20.17 w/w, 4.45 w/w, and 20 h, respectively. Under these conditions, the acid value as lauric acid enhanced 573% to 3.21 mg KOH/g oil. Storage stability attained through remaining activity on free lipase, PUF-lipase, PUF-co-immobilized-lipase were 9.89%, 42.3%, and 91.88%, respectively. In this study, the application of PUF-co-immobilized lipase in hydrolysis reactions can be reused up to 5 times. Characteristics of the addition of co-immobilized lipase have been analyzed using Fourier Transform Infra Red (FTIR) and Scanning Electron Microscope (SEM), showing the presence of functional groups binding and the changes in the surface matrix structure. Copyright © 2019 BCREC Group. All rights reserved

THE IMMOBILIZATION OF LIPASE FROM MUCOR MIEHEI ON ZEOLITE MATRIX IN HYDROLYSIS OF PALM OIL PRODUCING FREE FATTY ACIDS WITH SOLVENT FREE SYSTEM

The enzymatic hydrolysis of palm oil can be conducted by using lipase produced from Mucor miehei to produce free fatty acid. This study aimed to compare the usage of lipase as free enzyme and as immobilized enzyme on zeolite matrix in the hydrolysis of palm oil as triglyceride producing free fatty acids which highly needed in various industrial sectors. Immobilization is an alternative hydrolysis reaction due to its usage on repetitive reaction, makes lipase reuseable, hence the whole process becomes efficient, and with moderate operational conditions. Solvent free reaction is applied, because the produced free fatty acids can be used directly in food, health, and natural flavorings industry. The palm oil used in the hydrolysis contains 0.815% initial free fatty acids as palmitate, in which water then added to it in weight ratio 1:3. Each effect of free lipase and immobilized lipase addition is 4%, 5%, 6%, 7%, 8%, and time reaction is 30, 60, 90, 120, 150 minutes are used as index to determine the amount of free fatty acids produced. The results showed that Immobilized lipase has better ability than the free one in hydrolysis of triglyceride in palm oil producing free fatty acid with 8% lipase addition and time reaction of 120 minutes. Palm oil hydrolysis using free lipase produced the highest FFA of 1.9747% after the addition of 5% lipase concentrate, with time reaction of 60 minutes. Meanwhile, palm oil hydrolysis using immobilized lipase produced the highest FFA of 1.9747% after the addition of 8% lipase concentrate, with time reaction of 120 minutes.