Optimization of isoamyl acetate production by using immobilized lipase from Mucor miehei by response surface methodology

2000 ◽  
Vol 26 (2-4) ◽  
pp. 131-136 ◽  
Author(s):  
S Hari Krishna ◽  
B Manohar ◽  
S Divakar ◽  
S.G Prapulla ◽  
N.G Karanth
2015 ◽  
Vol 146 (12) ◽  
pp. 1949-1957 ◽  
Author(s):  
Shahrara Afshar ◽  
Maryam Sadehvand ◽  
Alireza Azad ◽  
Mohammad G. Dekamin ◽  
Mehdi Jalali-Heravi ◽  
...  

2012 ◽  
Vol 6 (1) ◽  
pp. 77-85
Author(s):  
Imen Aissa ◽  
Mohamed Sellami ◽  
Amel Kamoun ◽  
Youssef Gargouri ◽  
Nabil Miled

2012 ◽  
Vol 6 (1) ◽  
pp. 77-85 ◽  
Author(s):  
Imen Aissa ◽  
Mohamed Sellami ◽  
Amel Kamoun ◽  
Youssef Gargouri ◽  
Nabil Miled

2016 ◽  
Vol 4 (1) ◽  
pp. 56 ◽  
Author(s):  
Michael Osho ◽  
Tope Popoola ◽  
Tolulope Adeleye ◽  
Christianah Adetunji

<p>Optimization of Vegetable Sponge (<em>Luffa aegyptiaca</em>) (VS) - immobilization conditions of <em>Aspergillus niger</em> ATCC 1015 lipase on Solid State Fermentation (SSF) was carried out using Response Surface Methodology (RSM). Four independent variables (temperature, pH, enzyme loading and enzyme stability) were optimized using Central Composite Design of RSM for lipase production in a solid rice bran-physic nut cake medium. The optimal immobilization conditions obtained were 45 °C, pH 7.0, 2.5% (w/v) enzyme loading and 32.5% (v/v) enzyme stability (using glutaraldehyde as crosslinking agent) resulted into lipase activity of 98.6 Ug<sup>-1</sup>. The result demonstrates the potential application of vegetable sponge under SSF system in immobilizing lipase, thus contributed to efficiency of the use of this biomatrix as an immobilizing agent. The statistical tools employed predicted the optimal conditions for the production of the immobilized lipase thus revealing the full potential of the support.</p>


2017 ◽  
Vol 35 (4) ◽  
pp. 238-248 ◽  
Author(s):  
Ayoub Rashid Choudhry ◽  
Muhammad Mushtaq ◽  
Ahmad Adnan ◽  
Quratulain Syed

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