Biocatalytic behavior of a new Aspergillus niger whole-cell biocatalyst with high operational stability during the synthesis of green biosolvent isopropyl esters

2016 ◽  
Vol 131 ◽  
pp. 10-17 ◽  
Author(s):  
Zhiyou Pan ◽  
Shen Jin ◽  
Xi Zhang ◽  
Suiping Zheng ◽  
Shuangyan Han ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Operational Stability ◽  
Isopropyl Esters ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

Biodiesel fuel production by Aspergillus niger whole-cell biocatalyst in optimized medium

Mycoscience ◽  
2018 ◽  
Vol 59 (2) ◽  
pp. 147-152 ◽  
Author(s):  
Nailya R. Almyasheva ◽  
Maria I. Shuktueva ◽  
Daria A. Petrova ◽  
Dmitry S. Kopitsyn ◽  
Mikhail S. Kotelev ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Biodiesel Fuel ◽  
Fuel Production ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

Preparation of a Whole-Cell Biocatalyst of Aspergillus niger Lipase and Its Practical Properties

2010 ◽  
Vol 58 (19) ◽  
pp. 10426-10430 ◽  
Author(s):  
Wenshan Liu ◽  
Bin Jia ◽  
Heyun Zhao ◽  
Li Xu ◽  
Yunjun Yan
Keyword(s):  
Aspergillus Niger ◽  
Whole Cell ◽  
Whole Cell Biocatalyst

scholarly journals Tuning a bi-enzymatic cascade reaction in Escherichia coli to facilitate NADPH regeneration for ε-caprolactone production

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Jinghui Xiong ◽  
Hefeng Chen ◽  
Ran Liu ◽  
Hao Yu ◽  
Min Zhuo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Regeneration System ◽  
Nadph Regeneration ◽  
Whole Cell ◽  
Cyclohexanone Monooxygenase ◽  
Ribosome Binding ◽  
Ribosome Binding Sites ◽  
Whole Cell Biocatalyst ◽  
Substrate Tolerance

Abstractε-Caprolactone is a monomer of poly(ε-caprolactone) which has been widely used in tissue engineering due to its biodegradability and biocompatibility. To meet the massive demand for this monomer, an efficient whole-cell biocatalytic approach was constructed to boost the ε-caprolactone production using cyclohexanol as substrate. Combining an alcohol dehydrogenase (ADH) with a cyclohexanone monooxygenase (CHMO) in Escherichia coli, a self-sufficient NADPH-cofactor regeneration system was obtained. Furthermore, some improved variants with the better substrate tolerance and higher catalytic ability to ε-caprolactone production were designed by regulating the ribosome binding sites. The best mutant strain exhibited an ε-caprolactone yield of 0.80 mol/mol using 60 mM cyclohexanol as substrate, while the starting strain only got a conversion of 0.38 mol/mol when 20 mM cyclohexanol was supplemented. The engineered whole-cell biocatalyst was used in four sequential batches to achieve a production of 126 mM ε-caprolactone with a high molar yield of 0.78 mol/mol.

Antityrosinase Mechanism and Antimelanogenic Effect of Arbutin Esters Synthesis Catalyzed by Whole-Cell Biocatalyst

2021 ◽  
Vol 69 (14) ◽  
pp. 4243-4252
Author(s):  
Haixia Xu ◽  
Xiaofeng Li ◽  
Xuan Xin ◽  
Lan Mo ◽  
Yucong Zou ◽  
...  
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Engineered whole-cell biocatalyst-based detoxification and detection of neurotoxic organophosphate compounds

2014 ◽  
Vol 32 (3) ◽  
pp. 652-662 ◽  
Author(s):  
Chang Sup Kim ◽  
Jeong Hyun Seo ◽  
Dong Gyun Kang ◽  
Hyung Joon Cha
Keyword(s):  
Whole Cell ◽  
Organophosphate Compounds ◽  
Whole Cell Biocatalyst

Production of biodiesel by immobilized whole-cell biocatalyst

2008 ◽  
Vol 136 ◽  
pp. S385-S386
Author(s):  
Jyh-Ping Chen ◽  
Shu-Chin Chang
Keyword(s):  
Whole Cell ◽  
Whole Cell Biocatalyst

Permeabilizing Escherichia coli for whole cell biocatalyst with enhanced biotransformation ability from l-glutamate to GABA

2014 ◽  
Vol 107 ◽  
pp. 39-46 ◽  
Author(s):  
Wei-rui Zhao ◽  
Jun Huang ◽  
Chun-long Peng ◽  
Sheng Hu ◽  
Pi-yu Ke ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Whole Cell ◽  
Whole Cell Biocatalyst
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