Cloning and functional characterization of BcatrA, a gene encoding an ABC transporter of the plant pathogenic fungus Botryotinia fuckeliana (Botrytis cinerea)

The putative 4.5S RNA of Haemophilus influenzae was identified in the genome by computer analysis, amplified by the polymerase chain reaction, and cloned. We have determined that this putative 4.5S RNA will complement an Escherichia coli strain conditionally defective in 4.5S RNA production. The predicted secondary structures of the molecules were quite similar, but Northern analysis showed that the H. influenzae RNA was slightly larger than the E. coli RNA. The H. influenzae gene encoding this RNA is the functional homolog of the ffs gene in E. coli. Key words: ffs gene, complementation studies, small RNA, prokaryotic genetics.

Download Full-text

Isolation and functional characterization of the Ca-DREBLP1 gene encoding a dehydration-responsive element binding-factor-like protein�1 in hot pepper (Capsicum annuum L. cv. Pukang)

Planta ◽

10.1007/s00425-004-1412-5 ◽

2004 ◽

Vol 220 (6) ◽

pp. 875-888 ◽

Cited By ~ 77

Author(s):

Jong-Pil Hong ◽

Woo Taek Kim

Keyword(s):

Capsicum Annuum ◽

Functional Characterization ◽

Hot Pepper ◽

Gene Encoding ◽

Capsicum Annuum L ◽

Binding Factor ◽

Responsive Element

Download Full-text

Structural and functional characterization of a maltose/maltodextrin ABC transporter comprising a single solute binding domain (MalE) fused to the transmembrane subunit MalF

Research in Microbiology ◽

10.1016/j.resmic.2018.08.006 ◽

2019 ◽

Vol 170 (1) ◽

pp. 1-12 ◽

Cited By ~ 8

Author(s):

Anke Licht ◽

Martin Bommer ◽

Tobias Werther ◽

Kristin Neumann ◽

Carolin Hobe ◽

...

Keyword(s):

Abc Transporter ◽

Functional Characterization ◽

Binding Domain

Download Full-text

Characterization of an N-terminal Nav1.5 channel variant – a potential risk factor for arrhythmias and sudden death?

BMC Medical Genetics ◽

10.1186/s12881-020-01170-3 ◽

2020 ◽

Vol 21 (1) ◽

Author(s):

Stefanie Scheiper-Welling ◽

Paolo Zuccolini ◽

Oliver Rauh ◽

Britt-Maria Beckmann ◽

Christof Geisen ◽

...

Keyword(s):

Sudden Death ◽

Functional Characterization ◽

Missense Variant ◽

Hek293 Cells ◽

Patch Clamp Technique ◽

Gene Encoding ◽

Heterozygous Variant ◽

Cardiac Sodium Channel ◽

Electrophysiological Measurements

Abstract Background Alterations in the SCN5A gene encoding the cardiac sodium channel Nav1.5 have been linked to a number of arrhythmia syndromes and diseases including long-QT syndrome (LQTS), Brugada syndrome (BrS) and dilative cardiomyopathy (DCM), which may predispose to fatal arrhythmias and sudden death. We identified the heterozygous variant c.316A > G, p.(Ser106Gly) in a 35-year-old patient with survived cardiac arrest. In the present study, we aimed to investigate the functional impact of the variant to clarify the medical relevance. Methods Mutant as well as wild type GFP tagged Nav1.5 channels were expressed in HEK293 cells. We performed functional characterization experiments using patch-clamp technique. Results Electrophysiological measurements indicated, that the detected missense variant alters Nav1.5 channel functionality leading to a gain-of-function effect. Cells expressing S106G channels show an increase in Nav1.5 current over the entire voltage window. Conclusion The results support the assumption that the detected sequence aberration alters Nav1.5 channel function and may predispose to cardiac arrhythmias and sudden cardiac death.

Download Full-text