Autotransplantation of teeth: An alternative for patients with malignant transformation of oral submucous fibrosis

Background: Oral Submucous Fibrosis is one of the oral potentially malignant disorders presenting with progressive restriction in mouth opening. The condition having a high malignant transformation rate necessitates identification of biomarkers to be employed for early detection of malignant change. This will influence the prognosis in addition to adding better quality of life to patients.Aims and Objectives: To relate the association of VEGF -460C/T polymorphism in patients with OSMF and to compare the same among healthy subjects.Materials and Methods: Thirty patients with Oral submucous fibrosis and 20 controls free from habits and any form of lesions were included in the study. The polymorphism of VEGF gene was detected by polymerase chain reaction-based restriction analysis.Results: Sixty-four percent of the population in the study was in the age group of 21-40 yrs suggesting the prominence of disease in younger individuals with male predominance. With reference to polymorphism, 6.67% of the subjects from OSMF group showed CT polymorphism and 16.67% showed TT polymorphism. There were no statistically significant differences in the polymorphism between the study group and controls. However the frequency of T allele in the patient group 12 (20%) was greater than that in the control group 1 (2.5%), which was a significant finding. There was no association between the habits, frequency of habits, duration of quid placement, site of quid placement and style of chewing with the nature of polymorphism. Conclusion: VEGF 460C/T has the potential to be used as a prognostic marker in predicting the malignant transformation of OSMF.Asian Journal of Medical Sciences Vol.7(5) 2016 10-16

Download Full-text

Carcinogenesis of Male Oral Submucous Fibrosis Alters Salivary Microbiomes

Journal of Dental Research ◽

10.1177/0022034520968750 ◽

2020 ◽

pp. 002203452096875

Author(s):

M.Y. Chen ◽

J.W. Chen ◽

L.W. Wu ◽

K.C. Huang ◽

J.Y. Chen ◽

...

Keyword(s):

Malignant Transformation ◽

Oral Submucous Fibrosis ◽

Oral Microbiome ◽

Premalignant Lesions ◽

Rrna Gene ◽

Transformation Rate ◽

Validation Data ◽

Oral Carcinogenesis ◽

Key Species ◽

Submucous Fibrosis

Most oral squamous cell carcinoma (OSCC) tumors arise from oral premalignant lesions. Oral submucous fibrosis (OSF), usually occurring in male chewers of betel quid, is a premalignant stromal disease characterized by a high malignant transformation rate and high prevalence. Although a relationship between the inhabited microbiome and carcinogenesis has been proposed, no detailed information regarding the oral microbiome of patients with OSF exists; the changes of the salivary microbiome during cancer formation remain unclear. This study compared the salivary microbiomes of male patients with OSCC and a predisposing OSF background (OSCC-OSF group) and those with OSF only (OSF group). The results of high-throughput sequencing of the bacterial 16S rRNA gene indicated that OSF-related carcinogenesis and smoking status significantly contributed to phylogenetic composition variations in the salivary microbiome, leading to considerable reductions in species richness and phylogenetic diversity. The microbiome profile of OSF-related malignancy was associated with increased microbial stochastic fluctuation, which dominated the salivary microbiome assembly and caused species co-occurrence network collapse. Artificial intelligence selection algorithms consistently identified 5 key species in the OSCC-OSF group: Porphyromonas catoniae, Prevotella multisaccharivorax, Prevotella sp. HMT-300, Mitsuokella sp. HMT-131, and Treponema sp. HMT-927. Robust accuracy in predicting oral carcinogenesis was obtained with our exploratory and validation data sets. In functional analysis, the microbiome of the OSCC-OSF group had greater potential for S-adenosyl-l-methionine and norspermidine synthesis but lower potential for l-ornithine and pyrimidine deoxyribonucleotide synthesis and formaldehyde metabolism. These findings indicated that the salivary microbiome plays important roles in modulating microbial metabolites during oral carcinogenesis. In conclusion, our results provided new insights into salivary microbiome alterations during the malignant transformation of OSF.

Download Full-text

Oral submucous fibrosis: An update on pathophysiology of malignant transformation

Journal of Oral Pathology and Medicine ◽

10.1111/jop.12582 ◽

2017 ◽

Vol 46 (6) ◽

pp. 413-417 ◽

Cited By ~ 22

Author(s):

Gururaj Arakeri ◽

Shekar Gowda Patil ◽

Abdulsalam S. Aljabab ◽

Kuan-Chou Lin ◽

M. A. W. Merkx ◽

...

Keyword(s):

Malignant Transformation ◽

Oral Submucous Fibrosis ◽

Submucous Fibrosis

Download Full-text

Patients With Both Oral Leukoplakia and Oral Submucous Fibrosis Had a Higher Rate of Malignant Transformation Compared to Patients With Oral Leukoplakia or Oral Submucous Fibrosis Alone

Journal of Evidence Based Dental Practice ◽

10.1016/j.jebdp.2013.10.009 ◽

2013 ◽

Vol 13 (4) ◽

pp. 183-184 ◽

Cited By ~ 2

Author(s):

Michael W.S. Ho

Keyword(s):

Malignant Transformation ◽

Oral Submucous Fibrosis ◽

Oral Leukoplakia ◽

Submucous Fibrosis

Download Full-text

Hypoxia Inducible Factor-1α (HIF-1α): A Biomarker for Detection of Malignant Transformation in Oral Submucous Fibrosis

Pakistan Journal of Medicine and Dentistry ◽

10.36283/pjmd10-3/014 ◽

2021 ◽

Keyword(s):

Malignant Transformation ◽

Oral Submucous Fibrosis ◽

Hypoxia Inducible Factor ◽

Asian Population ◽

Hypoxia Inducible Factor 1Α ◽

Cell Stability ◽

Premalignant Condition ◽

Submucous Fibrosis ◽

And Function ◽

Upper Third

Oral submucous fibrosis (OSMF) is a premalignant condition, which involves the oral cavity as well as pharynx and upper third of the esophagus. It is a predominant disease of South and South-East Asian population. OSMF has the highest rate of malignant transformation among the other oral premalignant disorders (OPMD) that is 7-30% with a prevalence of about 0.03% to 6.42%. OSMF is described by extensive fibrosis of submucosa and reduced vascularity, which results in compromised blood supply guiding to hypoxia of tissue. This tissue hypoxia brings about the transcription of a set of genes associated with angiogenesis, breakdown of iron/glucose, cell division and cell stability. Hypoxia Inducible Factor-1α (HIF-1α) is the main biomarker intervening in this reaction. The alteration of cells to hypoxia emerges to be mediated through hypoxia inducible factor, which is said to be linked with malignant transformation of epithelium. Research-based studies covered on the probable mechanism of HIF-1α were explored using PubMed and Google Scholar. In this review, we analyzed over 40 articles from the years 2004-2021, which were published regarding the structure and function of HIF-1α, and its association with OSMF. Research articles in which HIF-1α is used separately or in combination with other biomarkers in oral submucous fibrosis have been selected. Keywords: Biomarker; Oral Submucous Fibrosis; Esophagus; Hypoxia.

Download Full-text