Overexpression of IbLfp in sweetpotato enhances the low-temperature storage ability of tuberous roots

Abstract Background: Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide, with 70-75% of production in China. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage. Results: For roots stored under 4℃ condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13℃). The activities of SOD, CAT, APX, O2.- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway (~22%), especially phenylpropanoids (~10%) and followed by starch and sucrose metabolism (~3%). Proteins including L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature (4℃) storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of sweetpotato roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Data are available via ProteomeXchange with identifier PXD017728.Conclusions: Our proteomic and metabolic profile analysis of sweetpotato roots stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (APX/CAT enzyme activity) data may be explained by higher H2O2 levels and increased glutathione and ascorbate redox states, which enhanced the CAT/APX enzyme activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways of sweetpotao roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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Transcriptome profiling of sweetpotato tuberous roots during low temperature storage

Plant Physiology and Biochemistry ◽

10.1016/j.plaphy.2016.12.021 ◽

2017 ◽

Vol 112 ◽

pp. 97-108 ◽

Cited By ~ 16

Author(s):

Chang Yoon Ji ◽

Won-Hyong Chung ◽

Ho Soo Kim ◽

Won Yong Jung ◽

Le Kang ◽

...

Keyword(s):

Low Temperature ◽

Transcriptome Profiling ◽

Low Temperature Storage ◽

Tuberous Roots ◽

Temperature Storage

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Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

10.21203/rs.2.22545/v5 ◽

2020 ◽

Author(s):

Peng Cui ◽

Yongxin Li ◽

Chenke Cui ◽

Yanrong Huo ◽

Guoquan Lu ◽

...

Keyword(s):

Low Temperature ◽

Metabolic Pathways ◽

Metabolic Profile ◽

Soluble Sugar ◽

Sucrose Metabolism ◽

Glucosinolate Biosynthesis ◽

Low Temperature Storage ◽

Leading Role ◽

Tuberous Roots ◽

Temperature Storage

Abstract Background: Sweetpotato ( Ipomoea batatas L.) is one of the seven major food crops grown worldwide, with 70-75% of production in China. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage. Results : For roots stored under 4℃ condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13 ℃). The activities of SOD, CAT, APX, O 2 .- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway (~22%), especially phenylpropanoids (~10%) and followed by starch and sucrose metabolism (~3%). Proteins including L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature (4℃) storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of sweetpotato roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Data are available via ProteomeXchange with identifier PXD017728. Conclusions: Our proteomic and metabolic profile analysis of sweetpotato roots stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (APX/CAT enzyme activity) data may be explained by higher H 2 O 2 levels and increased glutathione and ascorbate redox states, which enhanced the CAT/APX enzyme activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways of sweetpotao roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

10.21203/rs.2.22545/v7 ◽

2020 ◽

Author(s):

Peng Cui ◽

Yongxin Li ◽

Chenke Cui ◽

Yanrong Huo ◽

Guoquan Lu ◽

...

Keyword(s):

Low Temperature ◽

Metabolic Pathways ◽

Metabolic Profile ◽

Soluble Sugar ◽

Sucrose Metabolism ◽

Glucosinolate Biosynthesis ◽

Low Temperature Storage ◽

Leading Role ◽

Tuberous Roots ◽

Temperature Storage

Abstract Background: Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage. Results: For roots stored under 4℃ condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13℃). The activities of SOD, CAT, APX, O2.- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway, especially phenylpropanoids and followed by starch and sucrose metabolism. L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of roots. Leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Conclusions: Our proteomic and metabolic profile analysis of sweetpotatoes stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (CAT/APX activity) data may be explained by higher H2O2 levels and increased ascorbate redox states, which enhanced the CAT/APX activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways. Leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

BMC Plant Biology ◽

10.1186/s12870-020-02642-7 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Peng Cui ◽

Yongxin Li ◽

Chenke Cui ◽

Yanrong Huo ◽

Guoquan Lu ◽

...

Keyword(s):

Low Temperature ◽

Metabolic Pathways ◽

Profile Analysis ◽

Soluble Sugar ◽

Sucrose Metabolism ◽

Glucosinolate Biosynthesis ◽

Low Temperature Storage ◽

Leading Role ◽

Tuberous Roots ◽

Temperature Storage

Abstract Background Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage. Results For roots stored under 4 °C condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13 °C). The activities of SOD, CAT, APX, O2.- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway, especially phenylpropanoids and followed by starch and sucrose metabolism. L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of roots. Leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Conclusions Our proteomic and metabolic profile analysis of sweetpotatoes stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (CAT/APX activity) data may be explained by higher H2O2 levels and increased ascorbate redox states, which enhanced the CAT/APX activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways. Leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

10.21203/rs.2.22545/v1 ◽

2020 ◽

Author(s):

Peng Cui ◽

Yongxin Li ◽

Chenke Cui ◽

Yanrong Huo ◽

Guoquan Lu ◽

...

Keyword(s):

Low Temperature ◽

Metabolic Pathways ◽

Profile Analysis ◽

Soluble Sugar ◽

Sucrose Metabolism ◽

Glucosinolate Biosynthesis ◽

Low Temperature Storage ◽

Leading Role ◽

Tuberous Roots ◽

Temperature Storage

Abstract Background: Sweetpotato ( Ipomoea batatas L.) is one of the seven major food crops grown worldwide, with 70-75% of production in China.Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage. Results: For roots stored under 4℃ condition, the activities of SOD, CAT, APX, O 2 .- producing rate, proline and especially soluble sugar contents were significantly increased as compared to sweetpotatoes exposed to room temperature (13℃). Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway (~22%), especially phenylpropanoids (~10%) and followed by starch and sucrose metabolism (~3%). α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of sweetpotato roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Conclusions: Our proteomic and metabolic profile analysis of sweetpotato roots stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. Glucosinolate biosynthesis played a leading role in metabolic pathways of sweetpotao roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

10.21203/rs.2.22545/v2 ◽

2020 ◽

Author(s):

Peng Cui ◽

Yongxin Li ◽

Chenke Cui ◽

Yanrong Huo ◽

Guoquan Lu ◽

...

Keyword(s):

Low Temperature ◽

Metabolic Pathways ◽

Profile Analysis ◽

Soluble Sugar ◽

Sucrose Metabolism ◽

Glucosinolate Biosynthesis ◽

Low Temperature Storage ◽

Leading Role ◽

Tuberous Roots ◽

Temperature Storage

Abstract Background: Sweetpotato ( Ipomoea batatas L.) is one of the seven major food crops grown worldwide, with 70-75% of production in China.Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage. Results: For roots stored under 4℃ condition, the activities of SOD, CAT, APX, O 2 .- producing rate, proline and especially soluble sugar contents were significantly increased as compared to sweetpotatoes exposed to room temperature (13℃). Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway (~22%), especially phenylpropanoids (~10%) and followed by starch and sucrose metabolism (~3%). α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of sweetpotato roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Conclusions: Our proteomic and metabolic profile analysis of sweetpotato roots stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. Glucosinolate biosynthesis played a leading role in metabolic pathways of sweetpotao roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

10.21203/rs.2.22545/v4 ◽

2020 ◽

Author(s):

Peng Cui ◽

Yongxin Li ◽

Chenke Cui ◽

Yanrong Huo ◽

Guoquan Lu ◽

...

Keyword(s):

Low Temperature ◽

Metabolic Pathways ◽

Profile Analysis ◽

Soluble Sugar ◽

Sucrose Metabolism ◽

Glucosinolate Biosynthesis ◽

Low Temperature Storage ◽

Leading Role ◽

Tuberous Roots ◽

Temperature Storage

Abstract Background: Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide, with 70-75% of production in China. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage.Results: For roots stored under 4℃ condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13℃). The activities of SOD, CAT, APX, O2.- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway (~22%), especially phenylpropanoids (~10%) and followed by starch and sucrose metabolism (~3%). Proteins including L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature (4℃) storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of sweetpotato roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Data are available via ProteomeXchange with identifier PXD017728.Conclusions: Our proteomic and metabolic profile analysis of sweetpotato roots stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (APX/CAT enzyme activity) data may be explained by higher H2O2 levels and increased glutathione and ascorbate redox states, which enhanced the CAT/APX enzyme activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways of sweetpotao roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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Proteomic and metabolic profile analysis of low-temperature storage responses in Ipomoea batata Lam. tuberous roots

10.21203/rs.2.22545/v3 ◽

2020 ◽

Author(s):

Peng Cui ◽

Yongxin Li ◽

Chenke Cui ◽

Yanrong Huo ◽

Guoquan Lu ◽

...

Keyword(s):

Low Temperature ◽

Metabolic Pathways ◽

Profile Analysis ◽

Soluble Sugar ◽

Sucrose Metabolism ◽

Glucosinolate Biosynthesis ◽

Low Temperature Storage ◽

Leading Role ◽

Tuberous Roots ◽

Temperature Storage

Abstract Background: Sweetpotato (Ipomoea batatas L.) is one of the seven major food crops grown worldwide, with 70-75% of production in China. Cold stress often can cause protein expression pattern and substance contents variations for tuberous roots of sweetpotato during low-temperature storage. Recently, we developed proteometabolic profiles of the fresh sweetpotatoes (cv. Xinxiang) in an attempt to discern the cold stress-responsive mechanism of tuberous root crops during post-harvest storage.Results: For roots stored under 4℃ condition, the CI index, REC and MDA content in roots were significantly higher than them at control temperature (13℃). The activities of SOD, CAT, APX, O2.- producing rate, proline and especially soluble sugar contents were also significantly increased. Most of the differentially expressed proteins (DEPs) were implicated in pathways related to metabolic pathway (~22%), especially phenylpropanoids (~10%) and followed by starch and sucrose metabolism (~3%). Proteins including L-ascorbate peroxidase 3 and catalase were down-regulated during low temperature (4℃) storage. α-amylase, sucrose synthase and fructokinase were significantly up-regulated in starch and sucrose metabolism, while β-glucosidase, glucose-1-phosphate adenylyl-transferase and starch synthase were opposite. Furthermore, metabolome profiling revealed that glucosinolate biosynthesis, tropane, piperidine and pyridine alkaloid biosynthesis as well as protein digestion and absorption played a leading role in metabolic pathways of sweetpotato roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis. Data are available via ProteomeXchange with identifier PXD017728.Conclusions: Our proteomic and metabolic profile analysis of sweetpotato roots stored at low temperature reveal that the antioxidant enzymes activities, proline and especially soluble sugar content were significantly increased. Most of the DEPs were implicated in phenylpropanoids and followed by starch and sucrose metabolism. The discrepancy between proteomic (L-ascorbate peroxidase 3 and catalase) and biochemical (APX/CAT enzyme activity) data may be explained by higher H2O2 levels and increased glutathione and ascorbate redox states, which enhanced the CAT/APX enzyme activity indirectly. Glucosinolate biosynthesis played a leading role in metabolic pathways of sweetpotao roots. More importantly, leucine, tryptophan, tyrosine, isoleucine and valine were all significantly up-regulated in glucosinolate biosynthesis.

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