Use of fluorescent amplified fragment length polymorphism (fAFLP) to identify specific molecular markers for the biocontrol agent Aureobasidium pullulans strain LS30

Gummy stem blight (GSB) caused by the ascomycete fungus Didymella bryoniae (Auersw.) Rehm is an important disease of melon. Molecular markers linked to resistance would be useful for melon breeding programs. The amplified fragment length polymorphism (AFLP) technique and bulk segregant analysis were used to identify molecular markers linked to the resistance of melon to Didymella bryoniae. Segregation analysis of F2 progeny from a cross of PI 420145, a resistant line, and PI 136170, a susceptible line, showed that resistance to GSB was controlled by a dominant gene. One AFLP marker, E-TG/M-CTC200, was identified that is tightly linked to GSB resistance gene at a distance of 2.0 cM. To our best knowledge, this is the first report of AFLP markers linked to GSB resistance in melon. The identification of AFLP markers provides a step toward the use of marker-assisted selection and the characterization of the gene encoding resistance to GSB in melon.

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Intergeneric and Interspecific Relationships in Araceae tribe Caladieae and Development of Molecular Markers using Amplified Fragment Length Polymorphism (AFLP)

Annals of Botany ◽

10.1006/anbo.1999.1074 ◽

2000 ◽

Vol 85 (3) ◽

pp. 371-378 ◽

Cited By ~ 19

Author(s):

J Loh

Keyword(s):

Molecular Markers ◽

Length Polymorphism ◽

Fragment Length ◽

Fragment Length Polymorphism ◽

Amplified Fragment Length Polymorphism ◽

Interspecific Relationships

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Development of amplified fragment length polymorphism (AFLP) markers for the identification of Cholistani cattle

Archives Animal Breeding ◽

10.5194/aab-61-387-2018 ◽

2018 ◽

Vol 61 (4) ◽

pp. 387-394

Author(s):

Muhammad Haseeb Malik ◽

Muhammad Moaeen-ud-Din ◽

Ghulam Bilal ◽

Abdul Ghaffar ◽

Raja Danish Muner ◽

...

Keyword(s):

Molecular Markers ◽

Molecular Identification ◽

Length Polymorphism ◽

Fragment Length ◽

Fragment Length Polymorphism ◽

Amplified Fragment Length Polymorphism ◽

Snp Markers ◽

Crossbred Cattle ◽

Improvement Program ◽

Discrimination Ability

Abstract. The identification issue of livestock can be resolved by using molecular identification tools that are acceptable to preserve and maintain pure breeds worldwide. The application of a molecular identification methodology is more important for developing nations, e.g., Pakistan, where uncontrolled crossbreeding has become a common practice and the import of exotic animals and germplasm is ever increasing. This presents a risk to local breeds as also stated by the FAO. Therefore, the current study was designed to develop standard molecular markers for Cholistani cattle to ascertain their purity for breeding purpose. In this study 50 and 48 unrelated males were sampled for Cholistani and each crossbred cattle, respectively. Candidate molecular markers present in Cholistani but absent in crossbred cattle and vice versa were detected using the amplified fragment length polymorphism (AFLP) method. Eleven markers were developed and were converted to single nucleotide polymorphism (SNP) markers for genotyping. The allele frequencies in both breeds were determined for discrimination ability using polymerase-chain-reaction–restriction-fragment-polymorphism (PCR-AFLP). The probability of identifying the Cholistani breed was 0.905 and the probability of misjudgment was 0.073 using a panel of markers. The identified markers can ascertain the breed purity and are likely to extend the facility for breed purity testing before entering into a genetic improvement program in the country.

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