scholarly journals Detection of Enterococcus hirae in a case of acute osteomyelitis

2021 ◽  
Vol 16 (9) ◽  
pp. 2366-2369
Author(s):  
Rahul Bollam ◽  
Mohamed Yassin ◽  
Tung Phan
1998 ◽  
Vol 30 (6) ◽  
pp. 573-577 ◽  
Author(s):  
Lauritz B. Dahl ◽  
Anne-Lise Høyland ◽  
Harald Dramsdahl ◽  
Per Ivar Kaaresen

1999 ◽  
Vol 38 (07) ◽  
pp. 309-311 ◽  
Author(s):  
W. Brenner ◽  
H. Terheyden ◽  
K. H. Bohuslavizki ◽  
E. Henze ◽  
W. U. Kampen

SummaryThe accepted golden standard for detection of inflammatory bone disease is conventional three-phase bone scanning. Hyperperfusion, a high blood-pool activity and elevated bone metabolism are typical signs for an acute osteomyelitis. However, in case of subacute, chronic inflammation, neither elevated blood flow nor high blood-pool activity may be seen. This may cause difficulties in differentiating such cases from neoplastic or postoperative changes. This case report verifies the possible advantage of immunoscintigraphy with Tc-99m-labelled antigranulocyte Fab′-fragments (LeukoScan®) in a patient with infected mandibular osteoradionecrosis, who had equivocal clinical symptomes and questionable radiographic results. LeukoScan® is shown to be more sensitive in case of subacute bone inflammation compared with three-phase bone scanning. However, acquisition of delayed images after 24 hours including SPECT is inevitable in case of negative scans during the first hours of investigation.


2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  

Author(s):  
Nguyen Thanh Tam ◽  
Dương Thị Bé Ba ◽  
Nguyễn Thị Thùy Trang

Mục tiêu của nghiên cứu này nhằm đánh giá ảnh hưởng của việc bổ sung vi khuẩn Enterococcus hirae vào thức ăn lên khả năng tăng trưởng và tỷ lệ sống của cá tra giống. Thí nghiệm được bố trí hoàn toàn ngẫu nhiên với 3 nghiệm thức và mỗi nghiệm thức được lặp lại 3 lần. Nghiệm thức đối chứng (không bổ sung Enterococcus hirae), nghiệm thức 2 (bổ sung 30 ml dung dịch Enterococcus hirae/kg thức ăn) và nghiệm thức 3(bổ  sung 50 ml dung dịch Enterococcus hirae/kg thức ăn). Sử dụng thức ăn viên (Quaxcel-40N) để cho cá ăn. Thời gian thí nghiệm kéo dài trong 4 tuần. Kết quả cho thấy tỷ lệ sống của cá cao nhất (76,6%) ở nghiệm thức 3, thấp nhất (55,5%) ở nghiệm thức đối chứng và khác biệt có ý nghĩa (p < 0,05) giữa hai nghiệm thức này. Bên cạnh đó, khối lượng trung bình và chiều dài trung bình cuối của cá cao nhất (39,0g và 16,4 cm) ở nghiệm thức 3, kế đến là nghiệm thức 2 (37,2g và 16,1 cm) và khác biệt có ý nghĩa (p < 0,05) giữa hai nghiệm thức này so với nghiệm thức đối chứng. Như vậy, có thể bổ sung Enterococcus hirae vào thức ăn cho cá để đạt hiệu quả cao hơn.   Từ khóa: Cá tra giống, Enterococcus hirae, tăng trưởng, tỷ lệ sống, mật độ.


1994 ◽  
Vol 269 (15) ◽  
pp. 11037-11044
Author(s):  
K. Takase ◽  
S. Kakinuma ◽  
I. Yamato ◽  
K. Konishi ◽  
K. Igarashi ◽  
...  

2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S236-S237
Author(s):  
Nanda Ramchandar ◽  
Jessica Burns ◽  
Andrew Pennock ◽  
Christopher R Cannavino ◽  
Lauge Farnaes

Abstract Background Osteoarticular infections are often encountered in the pediatric population. Therapy is guided by isolation of a putative organism, however, operative cultures are often negative. Next generation sequencing (NGS) allows for more sensitive sampling of body compartments generally considered sterile. We sought to evaluate the utility of NGS in comparison to culture in detecting a pathogenic organism in acute osteomyelitis and septic arthritis in children. Methods This was a single-site study to evaluate the utility of NGS in comparison to culture in detecting a pathogenic organism in acute osteomyelitis and septic arthritis in children. Eligible patients were all patients with osteomyelitis or septic arthritis admitted to Rady Children’s Hospital from July 2019 through July 2020. We excluded any patients with bone or joint surgery within 30 days prior to admission. Operative samples were chosen at the surgeon’s discretion (joint aspirate, synovium, or bone) based on operative findings. We compared NGS testing to standard care culture from the same site. Results We enrolled 41 subjects. NGS of the operative samples identified a pathogen in 26 (63.4%) patients versus 18 (43.9%) by culture. Operative culture missed the diagnosis in 10 cases, though PCR identified the organism in 6 of those cases (5 were cases in which Kingella kingae was identified). In 4 subjects, NGS identified a putative organism where standard care testing (either PCR or culture) was negative. NGS was falsely positive in 1 subject and falsely negative for one other subject. Sensitivity was 96.3% (CI 95%, 81.0–99.9%) and Specificity was 92.9% (CI 95%, 66.1–99.8) for NGS versus 64.3% (CI 95%, 44.1–81.4) and 84.6% (CI 95%, 54.6–99.9%) for culture respectively. Conclusion In this single site prospective study of pediatric osteoarticular infections, we demonstrate improved sensitivity and specificity of NGS testing when compared to standard culture. Disclosures All Authors: No reported disclosures


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